三碘甲状腺原氨酸酶联免疫试剂盒(人) T3 ELISA KI

Triiodothyronine (T3) ELISA Kit — 三碘甲状腺原氨酸酶联免疫试剂盒 (人) KTE0006-3

产品分类：核心产品  |  产品中心：细胞分析  |  产品类型：ELISA  |  检测方法：竞争法

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一、产品概述

本套装基于EliKine™技术，专为血清、血浆及其他生物液体中总三碘甲状腺原氨酸（T3）进行定量检测而设计。采用竞争抑制原理，配合预包被T3抗体微孔板，实现 0.5 ~ 8 ng/mL 的线性检测范围，灵敏度达 0.4 ng/mL。

二、主要应用

• 蛋白类分析 —— 甲状腺功能筛选指标（T3）
• ELISA 应用 —— 研究型定量检测
• 适用于多物种样本：人、小鼠、大鼠、山羊、绵羊、狗、兔子、牛、猴子、鸡、猪

三、试剂盒组成

• T3 抗体预包被微孔板
• T3 标准品（多浓度梯度）
• 生物素偶联的 T3
• Avidin‑HRP 复合物
• HRP 底物 A 与 B（双底物体系）
• 反应终止液
• 洗涤缓冲液
• 封板膜（防潮）

四、技术特点 & 优势

• 高灵敏度与特异性：检测下限 0.4 ng/mL，能够准确捕获低浓度 T3。
• 低背景干扰：经验证对同族激素及相关蛋白的交叉反应极低。
• 兼容多种样本基质：血清、血浆以及其他生物液体均可直接使用。
• 操作简便：所有试剂均为预装配，遵循标准 ELISA 流程即可完成。

五、使用说明要点

1. 试剂不可与不同批号或已过期的试剂混用。
2. 使用前请将所有试剂在室温（约 20‑25 °C）平衡 30 分钟。
3. 实验过程中务必使用一次性吸头，防止交叉污染。
4. 未使用的板条请立即放回包装，密封后保存在 4 °C。
5. 充分混匀是获得可靠结果的关键，推荐每 10 分钟使用低速振荡器或手工轻摇。
6. 样品及标准品建议做双孔或三孔复测，提高数据可信度。

六、存储与运输

• 未开封试剂盒：2 ~ 8 °C 保存。
• 启用后各组分请参照说明书中具体保存要求。
• 运输方式：冷链（蓝冰）包装，确保全程低温。

七、规格与报价

规格	单价 (¥)	货期
48 T	1 598.00	期货（4 天内发货）
96 T	2 558.00	期货（4 天内发货）

八、法规声明

警告：本产品仅供研究用途，不得用于人类临床诊断。使用本套件进行的任何实验不得侵犯他人专利或许可。因使用本产品可能产生的专利侵权或其他违规责任，供货方不承担任何责任。

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九、背景信息（供参考）

血清中总 T3 与 T4 超过 99% 与甲状腺素结合球蛋白 (TBG) 结合，只有游离形式的 fT3 与 fT4 才能直接反映甲状腺功能状态。通过本 ELISA 试剂盒测定总 T3，可为甲状腺功能的基本筛选提供可靠的生化指标。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Triiodothyronine (T3) ELISA Kit — Human Triiodothyronine ELISA Kit (Human) KTE0006-3

Product Category: Core Product  |  Product Center: Cell Analysis  |  Product Type: ELISA  |  Detection Method: Competitive

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1. Product Overview

This kit is based on EliKine™ technology and is specifically designed for the quantitative detection of total triiodothyronine (T3) in serum, plasma, and other biological fluids. Using a competitive inhibition principle combined with a T3‑antibody‑coated microplate, it provides a linear detection range of 0.5 ~ 8 ng/mL with a sensitivity of 0.4 ng/mL.

2. Main Applications

• Protein analysis – Thyroid function screening marker (T3)
• ELISA application – Research‑grade quantitative detection
• Suitable for multiple species: human, mouse, rat, goat, sheep, dog, rabbit, cattle, monkey, chicken, pig

3. Kit Composition

• T3 antibody‑coated microplate
• T3 standards (multiple concentrations)
• Biotinylated T3
• Avidin‑HRP conjugate
• HRP substrate A and B (dual‑substrate system)
• Reaction stop solution
• Wash buffer
• Sealing film (moisture‑proof)

4. Technical Features & Advantages

• High sensitivity and specificity: detection limit of 0.4 ng/mL, accurately captures low‑level T3.
• Low background interference: demonstrated minimal cross‑reactivity with homologous hormones and related proteins.
• Compatible with various sample matrices: serum, plasma, and other biological fluids can be used directly.
• Simple operation: all reagents are pre‑assembled; follow standard ELISA procedures to complete the assay.

5. Key Points of Use

1. Do not mix reagents from different batches or expired reagents.
2. Allow all reagents to equilibrate at room temperature (≈ 20‑25 °C) for 30 minutes before use.
3. Use disposable pipette tips throughout the experiment to prevent cross‑contamination.
4. Unopened plate strips should be returned to their packaging immediately, sealed, and stored at 4 °C.
5. Thorough mixing is critical for reliable results; recommend gentle vortexing or low‑speed shaking every 10 minutes.
6. Run samples and standards in duplicate or triplicate to improve data reliability.

6. Storage & Transportation

• Unopened kits: store at 2 ~ 8 °C.
• After opening, refer to the instruction manual for specific storage conditions of each component.
• Transportation: cold‑chain (dry ice) packaging to ensure continuous low temperature.

7. Specifications & Pricing

Specification	Unit Price (¥)	Lead Time
48 T	1 598.00	Spot goods (shipment within 4 days)
96 T	2 558.00	Spot goods (shipment within 4 days)

8. Regulatory Statement

Warning: This product is for research use only and must not be used for human clinical diagnostics. Any experiments performed with this kit must not infringe on patents or licenses held by third parties. The supplier assumes no liability for patent infringement or other regulatory violations arising from the use of this product.

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9. Background Information (For Reference)

More than 99% of total T3 and T4 in serum are bound to thyroid‑binding globulin (TBG); only the free forms (fT3 and fT4) directly reflect thyroid functional status. Measuring total T3 with this ELISA kit provides a reliable biochemical indicator for basic thyroid function screening.

Note: The above information may change due to product optimization or upgrades. For the most current details, please contact Yakein Co., Ltd.