雌二醇酶联免疫试剂盒(驴) Estradiol ELISA

EliKine™ 雌二醇酶联免疫试剂盒(驴) Estradiol ELISA Kit

货号: KTE0003-B1-3 | 产品分类: 核心产品 | 产品中心: 细胞分析

产品概述

本试剂盒是一款基于竞争法原理的高品质ELISA检测试剂盒，专为精准定量检测多种生物样本（包括人、驴、小鼠、大鼠的血清、血浆及其他生物液体）中的雌二醇（Estradiol, E2）含量而设计。作为EliKine™系列的核心产品，本试剂盒在激素检测领域提供了可靠的解决方案，尤其适用于生殖内分泌、发育生物学及相关药理学研究。

检测技术参数

• 检测方法: 竞争法ELISA
• 检测指标: 雌二醇 (Estradiol, E2)
• 反应种属: 人、驴、小鼠、大鼠
• 样本类型: 血清、血浆、其他生物液体
• 检测范围: 40 pg/ml - 1000 pg/ml
• 灵敏度: 40 pg/ml

产品特点与优势

• 高灵敏度与特异性: 试剂盒经过优化，对目标物雌二醇表现出极高的检测灵敏度和特异性。在验证测试中，对待测物的结构类似物未观察到明显的交叉反应或背景干扰，有助于获得准确可靠的检测数据。
• 即用型组分: 试剂盒提供预包被抗体的微孔板及优化配比的试剂，简化实验流程，节省准备时间。
• 宽动态检测范围: 覆盖40 pg/ml至1000 pg/ml的宽范围，使其能够适应不同浓度水平的样本检测需求。
• 良好的种属兼容性: 已验证适用于人、驴、小鼠、大鼠等多种常见实验模型的样本检测。

试剂盒组分详情

• 雌二醇（Estradiol）抗体预包被微孔板
• 雌二醇（Estradiol）标准品系列
• HRP偶联的雌二醇（Estradiol）
• 雌二醇（Estradiol）检测抗体
• HRP底物溶液A
• HRP底物溶液B
• 反应终止液
• 浓缩洗涤缓冲液
• 封板膜

（注：具体组分含量请参考随货说明书。）

保存与运输

• 保存建议: 未开封的试剂盒请置于2-8℃保存。启用后，各组分请参照说明书建议的条件分别妥善保存。
• 运输条件: 全程采用冰袋（蓝冰）低温运输，以确保试剂活性和稳定性。

使用注意事项

• 请勿将不同批次的试剂或已过有效期的试剂混合使用。
• 所有试剂在使用前，建议室温平衡约30分钟。
• 实验操作过程中，请务必使用一次性吸头，并严格避免不同样品或试剂间的交叉污染。
• 实验中暂时不使用的微孔板条，应立即放回原包装袋中密封，并置于4℃保存。
• 充分的混匀步骤对实验结果的准确性至关重要，推荐使用低速频率振荡器，或每隔10分钟进行手动充分摇匀。
• 为获得更可靠的数据，建议对标准品和待测样品进行双孔或三孔（复孔）检测。

背景信息

雌二醇（Estradiol, E2），又称17β-雌二醇，是主要由卵巢成熟滤泡分泌的一种天然雌激素。其在生理上的核心功能包括促进子宫内膜增殖变化以及驱动女性第二性征的发育。血液中雌二醇的浓度是评估卵巢功能的重要指标，其水平在不同生理阶段（如排卵前期、排卵期、排卵后期）呈现规律性变化，浓度异常可能与卵巢功能低下、卵巢早衰等病理状况相关。

规格与价格

规格	价格 (元)	货期
48 T	1598.0	期货（预计4个工作日内发货）
96 T	2558.0	期货（预计4个工作日内发货）

重要声明

本产品仅限于研究用途，不适用于人类或临床诊断。 用户在使用本产品前，有责任确保其应用不违反任何相关的专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

搜索别名

雌二醇 ELISA试剂盒; 雌二醇; Estradiol ELISA试剂盒; Estradiol; E2; 17β-estradiol; Estrogen; KET0003

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Estradiol ELISA Kit (Donkey)

Catalog Number: KTE0003-B1-3 | Product Category: Core Products | Product Center: Cell Analysis

Product Overview

This kit is a high-quality ELISA detection kit based on the competitive principle, specifically designed for the accurate quantitative detection of estradiol (E2) levels in various biological samples (including serum, plasma, and other biological fluids from humans, donkeys, mice, and rats). As a core product of the EliKine™ series, this kit provides a reliable solution in the field of hormone detection, particularly suitable for reproductive endocrinology, developmental biology, and related pharmacological research.

Technical Parameters

• Assay Method: Competitive ELISA
• Target Analyte: Estradiol (E2)
• Reactive Species: Human, Donkey, Mouse, Rat
• Sample Types: Serum, Plasma, Other Biological Fluids
• Detection Range: 40 pg/ml - 1000 pg/ml
• Sensitivity: 40 pg/ml

Features & Advantages

• High Sensitivity and Specificity: The kit is optimized to exhibit extremely high detection sensitivity and specificity for the target analyte estradiol. In validation tests, no significant cross-reactivity or background interference was observed with structural analogs of the analyte, facilitating the acquisition of accurate and reliable detection data.
• Ready-to-Use Components: The kit provides pre-coated antibody microplates and optimally proportioned reagents, simplifying the experimental procedure and saving preparation time.
• Wide Dynamic Detection Range: Covers a broad range from 40 pg/ml to 1000 pg/ml, enabling adaptation to sample detection needs across different concentration levels.
• Excellent Species Compatibility: Validated for use with samples from various common experimental models including human, donkey, mouse, and rat.

Kit Components

• Estradiol Antibody Pre-coated Microplate
• Estradiol Standard Series
• HRP-conjugated Estradiol
• Estradiol Detection Antibody
• HRP Substrate Solution A
• HRP Substrate Solution B
• Stop Solution
• Wash Buffer Concentrate
• Plate Sealer

(Note: Please refer to the accompanying manual for specific component quantities.)

Storage and Shipping

• Storage Recommendation: Store the unopened kit at 2-8°C. After opening, store each component separately according to the conditions suggested in the manual.
• Shipping Conditions: Shipped with ice packs (blue ice) to ensure reagent activity and stability.

Precautions for Use

• Do not mix reagents from different batches or expired reagents.
• It is recommended to bring all reagents to room temperature and equilibrate for approximately 30 minutes before use.
• Use disposable pipette tips during the procedure and strictly avoid cross-contamination between different samples or reagents.
• Unused microplate strips should be immediately returned to the original packaging bag, sealed, and stored at 4°C.
• Adequate mixing is crucial for the accuracy of the results; use a low-frequency shaker or perform thorough manual shaking every 10 minutes.
• For more reliable data, it is recommended to perform duplicate or triplicate wells for standards and test samples.

Background Information

Estradiol (E2), also known as 17β-estradiol, is a natural estrogen primarily secreted by mature ovarian follicles. Its core physiological functions include promoting endometrial proliferation and driving the development of female secondary sexual characteristics. The concentration of estradiol in blood is an important indicator for assessing ovarian function. Its levels show regular changes during different physiological stages (such as pre-ovulation, ovulation, and post-ovulation phases). Abnormal concentrations may be associated with pathological conditions such as ovarian hypofunction and premature ovarian failure.

Specifications & Pricing

Specification	Price (CNY)	Lead Time
48 T	1598.0	Future supply (Expected shipment within 4 working days)
96 T	2558.0	Future supply (Expected shipment within 4 working days)

Important Notice

This product is intended for research use only, not for human or clinical diagnostic use. Users are responsible for ensuring that their application does not violate any relevant patent or licensing regulations. The company assumes no liability for any patent infringement or other violations that may result from the use of this product.

Search Aliases

Estradiol ELISA Kit; Estradiol; E2; 17β-estradiol; Estrogen; KET0003

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.