IL-5 ELISA KIT 白介素-5ELISA试剂盒(人

Human IL-5 ELISA Kit（人白介素-5 ELISA试剂盒）

货号：KTE6016-2

产品概述

本试剂盒为EliKine™系列核心产品，采用夹心法ELISA技术，专为精准检测人源样本中的白细胞介素-5（IL-5）设计。适用于细胞培养上清、血清、血浆及其他生物液体样本的分析，在免疫学研究、炎症反应评估及Th2细胞功能探索中具有重要应用价值。

关键特性与优势

• 高灵敏度：最低检测浓度达4 pg/mL，可精准捕捉低丰度IL-5表达。
• 优异特异性：经严格验证，与类似物无明显交叉反应，背景干扰低。
• 宽检测范围：7.8–500 pg/mL，覆盖生理及病理状态下IL-5的典型浓度区间。
• 稳定可靠：预包被板孔与优化试剂组分确保批间一致性，支持复孔检测以提升数据可靠性。

试剂盒组分

• 人源IL-5抗体预包被微孔板
• 人源IL-5蛋白标准品
• 人源IL-5检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液、实验缓冲液、HRP底物、反应终止液、洗涤缓冲液、封板膜

实验参数

反应性	人
样本类型	细胞培养上清、血清、血浆、其他生物液体
检测方法	夹心法ELISA
灵敏度	4 pg/mL
检测范围	7.8 pg/mL – 500 pg/mL

背景信息

白细胞介素-5（IL-5）是由115个氨基酸组成的Th2细胞因子，以同源二聚体形式发挥生物学功能。其主要由Th2细胞和肥大细胞分泌，通过促进B细胞增殖与免疫球蛋白分泌、激活嗜酸性粒细胞，参与变态反应性疾病（如哮喘、变应性鼻炎）的病理过程。UniProt编号：P05113。

订购信息

规格	价格（元）	货期
48 T	1598.0	现货（次日发货）
96 T	2558.0	现货（次日发货）

保存与运输

保存条件：未开封试剂盒需置于2–8℃；开封后请参照说明书分装保存各组分。
运输方式：冰袋（蓝冰）运输，确保试剂活性。

注意事项

• 请勿混用不同批号或过期试剂。
• 使用前需室温平衡30分钟，推荐使用一次性吸头避免污染。
• 未使用的板条应密封后4℃保存，实验过程中建议进行复孔检测以提高准确性。
• 样本与试剂需充分混匀，建议使用低速振荡器或定期手动摇匀。

警告：本产品仅用于科研，不适用于临床诊断或人体应用。使用前请查阅相关法规及专利限制。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IL-5 ELISA Kit (Human Interleukin‑5 ELISA Kit)

Catalog No.: KTE6016-2

Product Overview

This kit is a EliKine™ core product that utilizes a sandwich‑ELISA format, specifically designed for the accurate detection of human interleukin‑5 (IL‑5) in biological samples. It is suitable for analysis of cell‑culture supernatants, serum, plasma, and other biofluid specimens, and has important applications in immunology research, inflammation assessment, and Th2 cell‑function studies.

Key Features and Advantages

• High Sensitivity: Minimum detectable concentration of 4 pg/mL, enabling precise quantification of low‑abundance IL‑5.
• Excellent Specificity: Rigorously validated with negligible cross‑reactivity to related molecules and low background interference.
• Wide Dynamic Range: 7.8–500 pg/mL, covering typical physiological and pathological IL‑5 concentrations.
• Stable and Reliable: Pre‑coated plates and optimized reagent formulations ensure lot‑to‑lot consistency and support duplicate‑well testing for enhanced data reliability.

Kit Components

• Human IL‑5 antibody‑coated microplate
• Human IL‑5 recombinant protein standard
• Human IL‑5 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer, assay buffer, HRP substrate, stop solution, wash buffer, plate seal

Assay Parameters

Species	Human
Sample Types	Cell culture supernatant, serum, plasma, other biofluids
Assay Method	Sandwich ELISA
Sensitivity	4 pg/mL
Dynamic Range	7.8 pg/mL – 500 pg/mL

Background

Interleukin‑5 (IL‑5) is a Th2 cytokine composed of 115 amino acids that functions as a homodimer. It is primarily secreted by Th2 cells and mast cells and contributes to allergic diseases (e.g., asthma, allergic rhinitis) by promoting B‑cell proliferation and immunoglobulin secretion, as well as activating eosinophils. UniProt accession: P05113.

Ordering Information

Format	Price (CNY)	Lead Time
48 T	1598.0	In stock (next‑day shipment)
96 T	2558.0	In stock (next‑day shipment)

Storage and Shipping

Storage Conditions: Store unopened kits at 2–8 °C; after opening, aliquot and store individual components according to the user manual.
Shipping Method: Transport on ice packs (blue ice) to preserve reagent activity.

Precautions

• Do not mix reagents from different lots or use expired components.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use; use disposable tips to avoid contamination.
• Un‑used plates should be sealed and stored at 4 °C; duplicate‑well testing is recommended to improve accuracy.
• Ensure thorough mixing of samples and reagents; a low‑speed shaker or intermittent manual vortexing is advised.

Warning: This product is intended for research use only and is not suitable for clinical diagnosis or human application. Please consult relevant regulations and patent restrictions before use.

Note: The information above may be subject to change due to product optimizations or upgrades. For the most current details, please contact EliKine.