IL-1β ELISA KIT 白介素-1βELISA试剂盒

Human IL‑1β ELISA Kit (KTE6013‑2)

中文名称：IL‑1β ELISA KIT 白介素‑1β ELISA试剂盒（人）
货号：KTE6013‑2
检测指标：IL‑1β（人）
基因 ID：3553  蛋白质 ID：P01584
别名：IL1B, IL‑1, IL1‑BETA, IL1F2, catabolin, preinterleukin‑1 beta, pro‑interleukin‑1‑beta

---

产品概述

本套件为 EliKine™ 人白介素‑1β ELISA 定量试剂盒，采用夹心（sandwich）ELISA 体系，专为细胞培养上清、血清、血浆及其他生物液体中的 IL‑1β 检测设计。凭借高灵敏度与特异性，适用于炎症、免疫调节及细胞因子研究的基因表达与信号通路分析。

核心应用

• 细胞类研究 – 细胞因子分泌分析
• ELISA 应用 – 夹心法定量检测
• 细胞分析平台 – 实时监测炎症介导因子

试剂盒组成

• 预包被人源 IL‑1β 抗体的微孔板
• 人源 IL‑1β 蛋白标准品
• 人源 IL‑1β 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

性能规格

检测范围	灵敏度	检测方法
7.8 pg/mL – 500 pg/mL	4 pg/mL	夹心 ELISA（450 nm 微孔阅读）

样本类型

本试剂盒兼容以下样本基质：

• 细胞培养上清
• 血清
• 血浆
• 其他生物液体（如组织匀浆、体液）

实验操作要点

• 请勿混用不同批号或已过期的试剂。
• 所有试剂在使用前需在室温（≈25 °C）平衡 30 分钟。
• 操作期间建议使用一次性吸头，防止交叉污染。
• 未使用的板条应及时放回原包装，密闭保存于 4 °C。
• 充分混匀是保证结果一致性的关键；推荐使用低速振荡器或每 10 分钟手动摇匀一次。
• 样品和标准品建议设双孔或三孔复孔，以提高数据可靠性。

保存与运输

• 未开启包装：2 °C–8 °C（冰箱）保存。
• 开启后：请参照产品说明书对各组分分别保存。
• 运输条件：使用蓝色冰袋（冷链）运输，确保全程低温。

警示与免责声明

本产品仅供科研使用，**不适用于人体或临床诊断**。使用本套件进行的任何专利或许可证相关行为，需自行承担相应责任，厂商不承担因专利侵权或其他违规行为导致的后果。

技术背景

白介素‑1（IL‑1）是一类 17 kDa 的胞外多肽，包括 IL‑1α 与 IL‑1β 两种亚型。IL‑1β 由活化的巨噬细胞释放的前体经 Caspase‑1（CASP1/ICE）加工而成，是炎症反应的核心介导因子，参与细胞增殖、分化和凋亡等多种生物学过程。本套件针对人源 IL‑1β 设计，可帮助研究者在细胞水平准确量化该细胞因子的表达情况。

规格与订购信息

规格	单价 (人民币)	库存状态
48 T	1 598.0	现货（次日发货）
96 T	2 558.0	现货（次日发货）

检索关键词（便于搜索）

白介素‑1β ELISA试剂盒、IL‑1β ELISA、Human IL‑1β ELISA Kit、IL1B、IL‑1、IL1‑BETA、IL1F2、catabolin、preinterleukin‑1 beta、pro‑interleukin‑1‑beta、KET6013

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IL‑1β ELISA Kit (KTE6013‑2)

Chinese Name: IL‑1β ELISA KIT Human Interleukin‑1β ELISA Kit (Human)
Product No.: KTE6013‑2
Analyte: IL‑1β (Human)
Gene ID: 3553  Protein ID: P01584
Synonyms: IL1B, IL‑1, IL1‑BETA, IL1F2, catabolin, preinterleukin‑1 beta, pro‑interleukin‑1‑beta

---

Product Overview

This kit is the EliKine™ Human Interleukin‑1β ELISA Quantification Kit, employing a sandwich ELISA format specially designed for the detection of IL‑1β in cell culture supernatants, serum, plasma, and other biological fluids. With high sensitivity and specificity, it is suitable for gene expression and signaling pathway analyses in inflammation, immune modulation, and cytokine research.

Key Applications

• Cellular research – cytokine secretion analysis
• ELISA applications – sandwich quantitative assay
• Cell analysis platform – real-time monitoring of inflammatory mediators

Kit Components

• Microplate pre‑coated with human IL‑1β antibody
• Human IL‑1β protein standard
• Human IL‑1β detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer
• Assay buffer
• HRP substrate
• Reaction stop solution
• Wash buffer
• Plate sealing film

Performance Specifications

Detection Range	Sensitivity	Assay Method
7.8 pg/mL – 500 pg/mL	4 pg/mL	Sandwich ELISA (450 nm microplate reading)

Sample Types

This kit is compatible with the following sample matrices:

• Cell culture supernatants
• Serum
• Plasma
• Other biological fluids (e.g., tissue homogenates, body fluids)

Critical Experimental Steps

• Do not mix reagents from different lot numbers or expired reagents.
• All reagents should be equilibrated at room temperature (≈25 °C) for 30 minutes before use.
• Use disposable pipette tips during the procedure to prevent cross‑contamination.
• Unused strips should be returned to the original packaging promptly and stored sealed at 4 °C.
• Thorough mixing is critical for result consistency; we recommend using a low‑speed orbital shaker or manually vortexing every 10 minutes.
• Samples and standards are recommended to be run in duplicate or triplicate wells to improve data reliability.

Storage and Shipping

• Unopened: Store at 2 °C–8 °C (refrigerator).
• After opening: Store each component according to the product manual.
• Shipping conditions: Ship on dry ice or with blue cold packs (cold chain) to ensure continuous low temperature.

Warnings and Disclaimer

This product is for research use only and **is not intended for human or clinical diagnostic applications**. Any patent or license‑related activities performed using this kit are the responsibility of the user; the manufacturer assumes no liability for patent infringement or other violations.

Technical Background

Interleukin‑1 (IL‑1) comprises a family of extracellular 17 kDa peptides, including the IL‑1α and IL‑1β isoforms. IL‑1β is generated from a precursor released by activated macrophages and processed by Caspase‑1 (CASP1/ICE). It is a central mediator of inflammation and participates in cell proliferation, differentiation, and apoptosis. This kit is designed for human IL‑1β and enables researchers to accurately quantify this cytokine at the cellular level.

Specifications and Ordering Information

Format	Unit Price (CNY)	Stock Status
48 Test	1 598.0	In stock (next‑day shipping)
96 Test	2 558.0	In stock (next‑day shipping)

Search Keywords (for indexing)

Human Interleukin‑1β ELISA Kit, IL‑1β ELISA, Human IL‑1β ELISA Kit, IL1B, IL‑1, IL1‑BETA, IL1F2, catabolin, preinterleukin‑1 beta, pro‑interleukin‑1‑beta, KET6013

Note: The above information is subject to change due to product updates or other factors; please contact Yakeyin Corp. for the most current details.