IL-10 ELISA KIT 白介素-10ELISA试剂盒

产品概述

英文名称：Human IL-10 ELISA Kit 中文名称：IL-10 ELISA KIT 白介素-10 ELISA试剂盒(人)
货号：KTE6019-2 检测类型：ELISA（夹心法）
适用领域：细胞类研究 → ELISA应用 → 核心产品 → 细胞分析

核心检测指标

• 检测目标：人源白细胞介素-10 (IL-10)
• 基因ID：3586
• 蛋白质ID：P22301
• 别名：IL10, CSIF, TGIF, cytokine synthesis inhibitory factor 等

关键特性 & 优势

• 极高灵敏度：最低检测限 1 pg/mL，检测范围 2.35 pg/mL ~ 150 pg/mL。
• 特异性强：对IL-10同系物几乎无交叉反应，背景干扰低。
• EliKine™ 技术：采用预包被微孔板和高亲和力Streptavidin‑HRP，保证信号放大。
• 多样本兼容：细胞培养上清、血清、血浆及其他生物液体均可直接检测。

检测原理

本套件基于夹心ELISA原理：

1. 微孔板预包被针对人IL-10的捕获抗体。
2. 待测样品中的IL-10被捕获后，加入生物素化的检测抗体形成“双抗体复合物”。
3. EliKine™ Streptavidin‑HRP与检测抗体结合，加入HRP底物显色。
4. 使用酶标仪在450 nm读取吸光度，依据标准曲线定量样本中IL-10浓度。

检测范围 & 灵敏度

检测范围	2.35 pg/mL ~ 150 pg/mL
灵敏度 (LOD)	1 pg/mL

适用样本类型

本套件支持以下样本来源：

• 细胞培养上清
• 血清
• 血浆
• 其他生物液体（如组织匀浆、体液等）

试剂盒组分

• 人源IL-10抗体预包被微孔板
• 人源IL-10蛋白标准品
• 人源IL-10检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

使用指南

注意事项

• 不同批号或已超过有效期的试剂请勿混用。
• 使用前请让试剂在室温平衡30 分钟。
• 操作时使用一次性吸头，防止交叉污染。
• 未用完的板条请立即放回包装、密闭保存于4 ℃。
• 反应混合需充分均匀，建议使用低速振荡器或每10 分钟手动轻摇。
• 样品与标准品建议设置双孔或三孔复孔，以提高数据可靠性。

保存建议

• 未开启的试剂盒请存放于2‑8 ℃冰箱中。
• 开启后请参考说明书对各组分进行分装保存。

运输条件

采用蓝冰（冰袋）低温运输，确保全程保持冷链。

规格与报价

规格	单价（人民币）	供货状态
48 T	1598.0	现货（次日发货）
96 T	2558.0	现货（次日发货）

法规声明 & 警告

仅限科研使用：本产品不适用于人类临床诊断或治疗。

本套件的推荐用途不构成对任何专利或许可证的规避，使用过程中如涉及专利侵权或其他合规风险，责任由使用者自行承担。

技术背景

白细胞介素-10（IL-10）是一种具有免疫调节功能的细胞因子，由 Th2、胚胎胸腺细胞、单核/巨噬细胞、角质化细胞、B细胞及神经胶质细胞等多种细胞分泌。IL-10 通过抑制 NK 细胞和单核/巨噬细胞的活化，降低 IL-1、GM‑CSF、TNF、IL‑6、IL‑8、IL‑12、IFN‑γ 等促炎细胞因子的分泌，在炎症平衡、免疫耐受及自体免疫调控中发挥关键作用。

进一步信息

蛋白序列详情请访问：UniProt P22301

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Human IL-10 ELISA Kit Chinese Name: IL-10 ELISA KIT Interleukin‑10 ELISA reagent kit (human)
Catalog No.: KTE6019-2 Assay Type: ELISA (sandwich format)
Applicable Field: Cellular Research → ELISA Applications → Core Products → Cell Analysis

Key Analytical Parameters

• Detection Target: Human interleukin‑10 (IL‑10)
• Gene ID: 3586
• Protein ID: P22301
• Alias: IL10, CSIF, TGIF, cytokine synthesis inhibitory factor, etc.

Key Features & Advantages

• Ultra‑high sensitivity: Limit of detection 1 pg/mL; quantification range 2.35 pg/mL ~ 150 pg/mL.
• High specificity: Minimal cross‑reactivity with IL‑10 homologs and low background interference.
• EliKine™ technology: Utilizes pre‑coated microplates and high‑affinity Streptavidin‑HRP for signal amplification.
• Broad sample compatibility: Cell culture supernatants, serum, plasma, and other biological fluids can be assayed directly.

Assay Principle

This kit is based on the sandwich ELISA principle:

1. Microplate pre‑coated with a capture antibody specific for human IL‑10.
2. IL‑10 in the sample is captured, then a biotinylated detection antibody is added to form a “sandwich” complex.
3. EliKine™ Streptavidin‑HRP binds to the detection antibody, and HRP substrate is added for color development.
4. Absorbance is read at 450 nm on a microplate reader, and IL‑10 concentration is quantified using a standard curve.

Assay Range & Sensitivity

Assay Range	2.35 pg/mL ~ 150 pg/mL
Sensitivity (LOD)	1 pg/mL

Applicable Sample Types

The kit supports the following sample sources:

• Cell culture supernatant
• Serum
• Plasma
• Other biological fluids (e.g., tissue homogenates, body fluids, etc.)

Kit Components

• Human IL‑10 antibody pre‑coated microplate
• Human IL‑10 protein standard
• Human IL‑10 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer
• Assay buffer
• HRP substrate
• Reaction stop solution
• Wash buffer
• Sealing film

User Guide

Precautions

• Do not mix reagents from different lot numbers or past their expiration date.
• Allow reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips during operation to avoid cross‑contamination.
• Unutilized plate strips should be promptly returned to the package and stored sealed at 4 °C.
• Mix the reaction thoroughly; it is recommended to use a low‑speed shaker or gently vortex manually every 10 minutes.
• It is advisable to run samples and standards in duplicate or triplicate wells to improve data reliability.

Storage Recommendations

• Store unopened kits at 2‑8 °C in a refrigerator.
• After opening, refer to the instruction manual for aliquoting and storage of individual components.

Shipping Conditions

Shipped on dry ice (ice packs) with temperature control to maintain the cold chain throughout transit.

Specifications & Pricing

Format	Unit Price (CNY)	Availability
48 T	1598.0	In stock (next‑day shipment)
96 T	2558.0	In stock (next‑day shipment)

Regulatory Statement & Warning

For Research Use Only: This product is not intended for human clinical diagnosis or therapy.

The recommended uses of this kit do not constitute avoidance of any patents or licenses; users assume responsibility for any patent infringement or other compliance risks that may arise.

Technical Background

Interleukin‑10 (IL‑10) is an immunoregulatory cytokine secreted by various cell types, including Th2 cells, embryonic thymic cells, monocytes/macrophages, keratinocytes, B cells, and glial cells. IL‑10 suppresses activation of NK cells and monocytes/macrophages, reducing the secretion of pro‑inflammatory cytokines such as IL‑1, GM‑CSF, TNF, IL‑6, IL‑8, IL‑12, and IFN‑γ, thereby playing a pivotal role in inflammatory balance, immune tolerance, and auto‑immune regulation.

Further Information

For protein sequence details, visit: UniProt P22301

Note: The above information may change due to product optimization or upgrades; please contact Yakein Co. for the latest details.