Growth Hormone ELISA KIT 生长激素E

产品概览

英文名称：Human Growth Hormone ELISA Kit
中文名称：Growth Hormone ELISA KIT 生长激素ELISA试剂盒（人）
货号：KTE3094-2
检测类型：ELISA（夹心法）
适用样本：细胞培养上清、血清、血浆

核心应用领域

• 蛋白类分析
• ELISA研究应用
• 细胞分析中心的定量检测

试剂盒组成

• Human GH Microplate
• Human GH Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human GH Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

关键特性与优势

• 采用EliKine™双抗体夹心技术，提供高灵敏度（62.5 pg/mL）与宽检测范围（62.5 pg/mL – 4,000 pg/mL）。
• 对人类生长激素的同系物及相关蛋白无显著交叉反应，背景信号低。
• 标准品和检测抗体均为冻干形式，便于长期保存。

检测原理简述

本套件采用双抗体夹心法：

1. 微孔板预包被特异性抗人GH抗体。
2. 加入样品或标准品，待人GH与固相抗体结合。
3. 洗脱去除未结合组分。
4. 加入生物素化检测试剂抗体形成免疫复合物。
5. 加入链霉亲和素‑HRP，完成信号放大。
6. 加入TMB底物显色，终止液后在450 nm读取OD值。

操作注意事项

• 严禁混合不同批号或已过期的试剂。
• 使用前将所有试剂在室温平衡30 分钟。
• 实验过程请使用一次性吸头，防止交叉污染。
• 未用完的板条应立即放回包装，密闭后置于4 ℃保存。
• 混匀步骤建议使用低速振荡器或每10 分钟手动轻摇一次。
• 样品与标准建议设置双孔或三孔（复孔）以提高可靠性。

存储与运输

存储建议：未开封试剂盒避光保存于2 ℃–8 ℃，有效期24个月；开启后请依据说明书分别保存各组分。

运输条件：采用蓝冰（冰袋）冷链运输，确保全程温控。

警示与合规

本产品仅限科研使用，不可用于临床诊断或人类治疗。使用本试剂盒不应构成任何专利或许可证的侵犯，若因使用导致的专利纠纷或其他违规行为，供应方不承担责任。

产品背景

生长激素（GH）在人体和动物体内调控生长、细胞增殖与再生，是重要的应激激素。GH可提升血糖和游离脂肪酸水平，同时促进IGF‑1的合成。GH水平异常与垂体腺瘤、先天性发育障碍等疾病密切相关。对GH的精确定量有助于研究其生理功能及病理机制。

订购信息

规格	包装数量	单价（人民币）	交货周期
48 T	48 检测孔	1,598.00	期货（5 天内发货）
96 T	96 检测孔	2,558.00	期货（5 天内发货）

技术参数概览

• 检测指标：Growth Hormone
• 分子名称：Growth Hormone
• 反应物种：人
• 基因/蛋白信息：暂无

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Human Growth Hormone ELISA Kit
Chinese Name: Growth Hormone ELISA Kit (Human)
Product Number: KTE3094-2
Assay Type: ELISA (sandwich)
Applicable Samples: cell culture supernatant, serum, plasma

Core Application Areas

• Protein analysis
• ELISA research applications
• Quantitative assays in cell analysis cores

Kit Components

• Human GH Microplate
• Human GH Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human GH Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Key Features and Advantages

• Utilizes EliKine™ dual‑antibody sandwich technology, delivering high sensitivity (62.5 pg/mL) and a broad detection range (62.5 pg/mL – 4,000 pg/mL).
• Shows negligible cross‑reactivity with GH isoforms and related proteins, resulting in low background signal.
• Both standards and detection antibodies are supplied in lyophilized form for long‑term storage.

Brief Description of Assay Principle

This kit employs a dual‑antibody sandwich format:

1. The microplate is pre‑coated with a specific anti‑human GH capture antibody.
2. Samples or standards are added, allowing human GH to bind to the solid‑phase antibody.
3. Unbound components are washed away.
4. A biotinylated detection antibody is added to form an immune complex.
5. Streptavidin‑HRP is introduced to complete signal amplification.
6. Trimethylbenzidine (TMB) substrate is added for color development; the reaction is stopped and the absorbance is read at 450 nm.

Operational Precautions

• Do not mix reagents from different batches or past their expiration dates.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Unused plate strips should be returned to their packaging immediately, sealed, and stored at 4 °C.
• Mixing steps are best performed with a low‑speed shaker or by gently inverting the plate every 10 minutes.
• Run samples and standards in duplicate or triplicate (replicates) to improve reliability.

Storage and Shipping

Storage Recommendation: Store unopened kits protected from light at 2 °C–8 °C; shelf life 24 months. After opening, store individual components as described in the user manual.

Shipping Conditions: Transport on a cold chain using ice packs (“blue ice”) to maintain temperature control throughout transit.

Warnings and Compliance

This product is for research use only and must not be employed for clinical diagnosis or human therapy. Use of this kit does not constitute infringement of any patents or licenses; the supplier assumes no liability for patent disputes or other violations arising from its use.

Product Background

Growth hormone (GH) regulates growth, cellular proliferation, and regeneration in humans and animals and is a key stress hormone. GH elevates blood glucose and free fatty acid levels while promoting IGF‑1 synthesis. Abnormal GH concentrations are closely associated with pituitary adenomas, congenital developmental disorders, and other diseases. Accurate quantification of GH facilitates investigation of its physiological roles and pathological mechanisms.

Ordering Information

Specification	Package Quantity	Unit Price (CNY)	Delivery Lead Time
48 T	48 assay wells	1,598.00	Spot (shipped within 5 days)
96 T	96 assay wells	2,558.00	Spot (shipped within 5 days)

Technical Specifications Overview

• Analyte: Growth Hormone
• Molecule Name: Growth Hormone
• Species Reactivity: Human
• Gene/Protein Information: Not available

Note: The above information is subject to change due to product optimization or upgrades; for the most current details, please contact Yakeyin Co.