人趋化因子配体13 ELISA定量试剂盒(CXCL13/BL

Human CXCL13/BLC/BCA-1 ELISA Kit (KTE3101-1)

产品概览

中文名称：人趋化因子配体13 ELISA定量试剂盒 (CXCL13/BLC/BCA-1)
英文名称：Human CXCL13/BLC/BCA-1 ELISA Kit
货号：KTE3101-1
核心技术：EliKine™ 双抗体夹心ELISA平台

适用范围

• 蛋白类研究 – CXCL13（亦称BLC、BCA-1）定量检测
• 一级应用：ELISA（酶联免疫吸附测定）
• 细胞分析中心：细胞培养上清、血清、血浆样本
• 研究用途：基础免疫学、肿瘤免疫、B细胞和Tfh细胞功能研究

检测原理

采用双抗体夹心法，板上预包被特异性抗CXCL13抗体。样品或标准加入后，CXCL13与固相抗体结合；随后加入生物素化检测抗体、链霉亲和素‑HRP复合物，形成可被TMB底物显色的免疫复合物。450 nm波长读取的OD值与CXCL13浓度呈正比。

检出范围与灵敏度

• 检测范围：31.25 pg/mL – 2,000 pg/mL
• 最低检出限 (LOD)：31.25 pg/mL

试剂盒组成

• Human CXCL13 Microplate
• Human CXCL13 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human CXCL13 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

优势特点

• 高灵敏度：能够检测至31.25 pg/mL。
• 特异性强：未观察到显著的交叉反应或背景干扰。
• 适用样本广：细胞培养上清、血清、血浆均可直接检测。
• 双孔/三孔复检推荐，提升数据可靠性。

使用注意事项

• 不同批号或已过期的试剂不得混用。
• 使用前在室温平衡30 分钟。
• 实验操作请使用一次性吸头，防止交叉污染。
• 未用完的板条请立即放回包装，密闭保存于4 °C。
• 混匀步骤至关重要，推荐低速振荡器或每10 分钟手动轻摇。
• 样品与标准建议做双孔或三孔复孔检测。

保存与运输

• 未开封试剂盒：避光保存于2 °C–8 °C，保质期24个月。
• 已开封组分：请参照说明书分别保存。
• 运输条件：冰袋（蓝冰）配冷链运输。

警告声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套件进行的任何专利相关活动均由使用者自行承担风险，供应商不承担相应责任。

技术背景

CXCL13（亦称BLC/BCA-1）在肝、脾、淋巴结及肠道中高表达，主要通过CXCR5受体调控B细胞在淋巴滤泡中的定位和功能。其在滤泡辅助性T细胞（Tfh）以及T细胞淋巴瘤中的表达，可作为免疫微环境及肿瘤来源的生物标记。本ELISA套件为研究CXCL13在免疫调控、疾病模型以及药物干预中的动态变化提供定量工具。

产品规格与报价

规格	单价 (CNY)	交货期
48 T	1,598.00	期货（5天内发货）
96 T	2,558.00	期货（5天内发货）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human CXCL13/BLC/BCA-1 ELISA Kit (KTE3101-1)

Product Overview

Chinese Name: Human Chemokine Ligand 13 ELISA Quantitation Kit (CXCL13/BLC/BCA-1)
English Name: Human CXCL13/BLC/BCA-1 ELISA Kit
Catalog No.: KTE3101-1
Core Technology: EliKine™ Dual-Antibody Sandwich ELISA Platform

Scope of Application

• Protein research – Quantitative detection of CXCL13 (also known as BLC, BCA-1)
• Primary application: ELISA (Enzyme‑Linked Immunosorbent Assay)
• Cell analysis core: Cell culture supernatants, serum, plasma samples
• Research uses: Fundamental immunology, tumor immunology, B‑cell and Tfh‑cell functional studies

Assay Principle

The assay utilizes a dual‑antibody sandwich format with plates pre‑coated with a specific anti‑CXCL13 capture antibody. Upon addition of sample or standard, CXCL13 binds to the solid‑phase antibody; subsequently, a biotinylated detection antibody and streptavidin‑HRP conjugate are added, forming an immune complex that reacts with TMB substrate to produce color. The optical density measured at 450 nm is directly proportional to the CXCL13 concentration.

Detection Range and Sensitivity

• Detection Range: 31.25 pg/mL – 2,000 pg/mL
• Limit of Detection (LOD): 31.25 pg/mL

Kit Components

• Human CXCL13 Microplate
• Human CXCL13 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human CXCL13 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Key Advantages

• High sensitivity: Capable of detecting down to 31.25 pg/mL.
• Strong specificity: No significant cross‑reactivity or background interference observed.
• Broad sample compatibility: Cell culture supernatants, serum, and plasma can be assayed directly.
• Recommended duplicate or triplicate wells to enhance data reliability.

Precautions

• Do not mix reagents from different lots or expired batches.
• Allow the kit to equilibrate at room temperature for 30 minutes before use.
• Use disposable pipette tips during procedures to avoid cross‑contamination.
• Return any unused plate strips to their original packaging immediately and store sealed at 4 °C.
• Mixing is critical; recommended to use a low‑speed vortex or gently invert manually every 10 minutes.
• Perform sample and standard analyses in duplicate or triplicate wells.

Storage and Shipping

• Unopened kit: Store protected from light at 2 °C–8 °C; shelf life 24 months.
• Opened components: Store according to the instructions provided for each item.
• Shipping conditions: Shipped on ice packs (blue ice) with cold‑chain logistics.

Warning Statement

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Any patent‑related activities performed with this kit are undertaken at the user’s own risk; the supplier assumes no liability.

Technical Background

CXCL13 (also known as BLC/BCA-1) is highly expressed in liver, spleen, lymph nodes, and the intestine, where it primarily regulates B‑cell positioning and function within lymphoid follicles via the CXCR5 receptor. Its expression in follicular helper T cells (Tfh) and T‑cell lymphomas serves as a biomarker of the immune microenvironment and tumor origin. This ELISA kit provides a quantitative tool for investigating the dynamic changes of CXCL13 in immune regulation, disease models, and therapeutic interventions.

Product Specifications and Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.00	Stock (ship within 5 days)
96 T	2,558.00	Stock (ship within 5 days)

Note: The above information may change due to product optimization, upgrades, or other factors. For the most current details, please contact Yakein Co.