大鼠血小板衍生生长因子BB ELISA定量试剂盒(PDGF-

人/大鼠血小板衍生生长因子BB (PDGF-BB) ELISA定量试剂盒

货号：KTE3060-B1-1

EliKine™ 大鼠血小板衍生生长因子BB ELISA定量试剂盒是一款高品质的核心产品，专为细胞分析领域设计。本试剂盒采用先进的夹心法ELISA技术，可精准定量检测细胞培养上清、血清及血浆样本中的人源与大鼠源PDGF-BB含量，为您的生命科学研究提供可靠的数据支持。

产品概述

血小板衍生生长因子BB (PDGF-BB) 是一种重要的生长因子，在血管生成、组织修复及细胞增殖等关键生物过程中扮演着核心角色。本试剂盒具有极高的灵敏度和特异性，检测范围广，为研究相关生理及病理过程提供了强有力的工具。

产品特点与优势

• 高灵敏度：最低检测浓度可达31.25 pg/mL。
• 宽检测范围：检测范围为31.25 pg/mL至2,000 pg/mL，适用于多种浓度水平的样本检测。
• 优异特异性：经严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰。
• 即用型设计：提供预包被微孔板及优化好的缓冲液体系，操作流程简便。

试剂盒组分

• Human/Rat PDGF-BB Microplate (预包被微孔板)
• Human/Rat PDGF-BB Standard (冻干标准品)
• 标准品稀释液
• Assay Buffer (10×) （检测缓冲液）
• Human/Rat PDGF-BB Detect Antibody (100×) （检测抗体）
• Streptavidin-HRP (100×) （链霉亲和素-HRP）
• HRP Substrate (TMB) （HRP底物）
• Stop Solution （终止液）
• Wash Buffer (20×) （洗涤缓冲液）
• Plate Covers （板盖）

技术参数

参数	详情
产品类型	ELISA试剂盒
检测方法	夹心法ELISA
反应性	人、大鼠
样本类型	细胞培养上清、血清、血浆
检测范围	31.25 - 2,000 pg/mL
灵敏度	31.25 pg/mL
规格与价格	48 T: 1598.0元 | 96 T: 2558.0元 (货期请咨询)

使用说明

重要注意事项：

• 请勿将不同批号或已过期的试剂混合使用。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 实验过程中充分的混匀至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更准确的结果，建议对样品和标准品进行复孔（双孔或三孔）检测。

保存与运输

• 保存建议：未开封的试剂盒请避光保存于2-8℃，保质期为24个月。启用后，请参照说明书分别保存各组分。
• 运输条件：本品采用冰袋（蓝冰）运输，以确保试剂活性。

背景信息

血小板衍生生长因子(PDGF)是由A链和B链组成的二聚体糖蛋白，其中PDGF-BB在血管生成、创伤愈合及骨骼形成等过程中发挥关键作用。其异常表达与多种疾病，特别是肿瘤的发生发展密切相关。本试剂盒基于双抗体夹心法原理，能够特异性地捕获并检测样本中的PDGF-BB蛋白，通过HRP催化的显色反应进行定量分析。

警告： 本产品仅供研究使用，不适用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human/Rat Platelet-Derived Growth Factor BB (PDGF-BB) ELISA Quantification Kit

Catalog Number: KTE3060-B1-1

The EliKine™ Rat Platelet-Derived Growth Factor BB ELISA Quantification Kit is a high-quality core product specifically designed for cell analysis applications. Utilizing advanced sandwich ELISA technology, this kit enables precise quantitative detection of human and rat PDGF-BB levels in cell culture supernatants, serum, and plasma samples, providing reliable data support for your life science research.

Product Overview

Platelet-Derived Growth Factor BB (PDGF-BB) is a crucial growth factor that plays a central role in key biological processes such as angiogenesis, tissue repair, and cell proliferation. This kit offers high sensitivity and specificity with a broad detection range, providing a powerful tool for studying related physiological and pathological processes.

Features & Advantages

• High Sensitivity: Minimum detectable concentration reaches 31.25 pg/mL.
• Broad Detection Range: Detection range from 31.25 pg/mL to 2,000 pg/mL, suitable for samples with various concentration levels.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with analogs of the target protein.
• Ready-to-Use Design: Provides pre-coated microplate and optimized buffer system for simplified operation.

Kit Components

• Human/Rat PDGF-BB Microplate (Pre-coated)
• Human/Rat PDGF-BB Standard (Lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human/Rat PDGF-BB Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Technical Parameters

Parameter	Details
Product Type	ELISA Kit
Detection Method	Sandwich ELISA
Reactivity	Human, Rat
Sample Type	Cell Culture Supernatant, Serum, Plasma
Detection Range	31.25 - 2,000 pg/mL
Sensitivity	31.25 pg/mL
Size & Price	48 T: 1598.0 CNY | 96 T: 2558.0 CNY (Delivery time please inquire)

Instructions for Use

Important Notes:

• Do not mix reagents from different lots or expired reagents.
• All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the procedure to avoid cross-contamination.
• Unused strips should be returned to the original pouch immediately and sealed, stored at 4°C.
• Thorough mixing during the procedure is crucial; use a low-speed shaker or shake manually periodically.
• For more accurate results, it is recommended to test samples and standards in replicates (duplicate or triplicate).

Storage & Shipping

• Storage Recommendation: Store the unopened kit protected from light at 2-8°C. Shelf life is 24 months. After opening, refer to the instructions for storage of individual components.
• Shipping Conditions: This product is shipped with ice packs (blue ice) to ensure reagent activity.

Background Information

Platelet-Derived Growth Factor (PDGF) is a dimeric glycoprotein composed of A and B chains, among which PDGF-BB plays a key role in processes such as angiogenesis, wound healing, and bone formation. Its aberrant expression is closely associated with the development and progression of various diseases, particularly tumors. Based on the principle of double antibody sandwich ELISA, this kit specifically captures and detects PDGF-BB protein in samples, enabling quantitative analysis through an HRP-catalyzed colorimetric reaction.

Warning: This product is for research use only, not intended for human or clinical diagnostic use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yaken Company for specific details.