人成纤维细胞生长因子7 ELISA定量试剂盒(KGF/FGF

Human KGF/FGF‑7 ELISA Kit (KTE3052‑1)

产品概览

• 中文名称：人成纤维细胞生长因子7 ELISA定量试剂盒 (KGF/FGF‑7)
• 英文名称：Human KGF/FGF‑7 ELISA Kit
• 货号：KTE3052‑1
• 产品分类：核心产品 → 细胞分析 → ELISA
• 检测类型：酶联免疫吸附试验（ELISA）— 夹心法
• 适用种属：人（Human）
• 可检测样本：细胞培养上清、血清、血浆

技术参数

检测范围	31.25 pg/mL – 2,000 pg/mL
灵敏度（LOD）	31.25 pg/mL
分子/检测指标	KGF/FGF‑7
别名	KGF / FGF‑7

试剂盒组成（每盒）

• Human KGF Microplate
• Human KGF Standard (冻干粉)
• Standard Diluent
• Assay Buffer (10×)
• Human KGF Detect Antibody (100×, biotin‑标记)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

核心优势

• 高灵敏度 & 高特异性：EliKine™ 采用双抗体夹心结构，背景噪声低，未检测到显著交叉反应。
• 宽检测范围：31.25 pg/mL–2,000 pg/mL，覆盖大多数研究需求。
• 兼容多种基质：细胞培养上清、血清及血浆均可直接检测。

实验流程要点（简要）

1. 将人KGF特异性捕获抗体预包被于酶标板孔中。
2. 加入样品或标准品，KGF 与捕获抗体形成第一层复合物。
3. 洗涤后加入生物素化检测抗体，形成“捕获‑KGF‑检测”三联结构。
4. 加入链霉亲和素‑HRP，随后洗涤去除未结合酶。
5. 加入TMB底物，HRP 催化产生蓝色，终止液使之转为黄色。
6. 在 450 nm 处读取 OD 值，使用标准曲线换算浓度。

使用注意事项

• 试剂仅能与同批号、在有效期内的试剂混用；不同批号或过期试剂请勿混合。
• 使用前让所有试剂在室温平衡 30 分钟。
• 实验全程建议使用一次性吸头，防止交叉污染。
• 未使用的板条应立即放回原包装，密闭保存于 4 ℃。
• 充分混匀对结果影响显著，推荐使用低速频率振荡器或每 10 分钟手动轻摇。
• 样品和标准最好设置双孔或三孔（复孔）以提高精密度。

保存与运输

• 未开封保存：避光、2–8 ℃，保质期 24 个月。
• 启用后：请参照说明书对各组分分别保存。
• 运输条件：冷链（蓝冰）包装，确保全程低温。

警示声明

本产品仅限科研用途，不用于临床诊断或人体治疗。使用过程中如涉及专利或许可证，请自行确认合规性，供应商不承担因侵权或违规产生的任何责任。

产品包装 & 价格

规格	单价（人民币）	交货周期
48 T	¥1,598.0	期货（5 天内发货）
96 T	¥2,558.0	期货（5 天内发货）

科研背景

角质细胞生长因子（KGF/FGF‑7）是伤口再上皮化阶段的重要信号分子，能够通过与 FGFR2b 受体结合，诱导上皮细胞增殖与分化。炎症因子（IL‑1α、IL‑1β、IL‑6）可上调成纤维细胞的 KGF 表达，进而促进伤口愈合。采用本套ELISA 试剂盒，可在细胞培养上清、血清或血浆中高灵敏度定量 KGF，帮助揭示其在细胞通讯、组织修复及药物干预中的功能机制。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human KGF/FGF‑7 ELISA Kit (KTE3052‑1)

Product Overview

• Chinese Name: Human Keratinocyte Growth Factor 7 ELISA Quantitative Kit (KGF/FGF‑7)
• English Name: Human KGF/FGF‑7 ELISA Kit
• Catalog No.: KTE3052‑1
• Category: Core Products → Cell Analysis → ELISA
• Assay Type: Enzyme‑Linked Immunosorbent Assay (ELISA) – Sandwich
• Species: Human
• Sample Types: Cell culture supernatants, serum, plasma

Technical Specifications

Detection Range	31.25 pg/mL – 2,000 pg/mL
Sensitivity (LOD)	31.25 pg/mL
Analyte	KGF/FGF‑7
Synonyms	KGF / FGF‑7

Kit Components (per kit)

• Human KGF Microplate
• Human KGF Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human KGF Detect Antibody (100×, biotin‑labeled)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Key Advantages

• High Sensitivity & High Specificity: EliKine™ utilizes a dual‑antibody sandwich format, low background noise, and no significant cross‑reactivity detected.
• Wide Dynamic Range: 31.25 pg/mL–2,000 pg/mL, covering most research needs.
• Matrix Compatibility: Directly applicable to cell culture supernatants, serum, and plasma.

Brief Experimental Procedure

1. Pre‑coat ELISA plate wells with human KGF‑specific capture antibody.
2. Add samples or standards; KGF binds to the capture antibody forming the first complex.
3. After washing, add biotinylated detection antibody to create a “capture‑KGF‑detector” sandwich.
4. Add streptavidin‑HRP, then wash to remove unbound enzyme.
5. Add TMB substrate; HRP catalyzes a blue color, which is converted to yellow by the stop solution.
6. Read absorbance at 450 nm and calculate concentrations using the standard curve.

Precautions

• Reagents may only be mixed with those from the same lot and within the expiration date; do not mix different lots or expired reagents.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the assay to prevent cross‑contamination.
• Unutilized plate strips should be returned to their original packaging promptly and stored sealed at 4 °C.
• Thorough mixing markedly affects results; a low‑speed vortex or gentle hand shaking every 10 minutes is recommended.
• Run samples and standards in duplicate or triplicate wells to improve precision.

Storage & Shipping

• Unopened Storage: Protect from light, 2–8 °C, shelf life 24 months.
• After Opening: Follow the manual for individual component storage.
• Shipping Conditions: Cold chain (blue ice) packaging to maintain low temperature throughout transit.

Warning Statement

This product is for research use only and is not intended for clinical diagnosis or human therapy. Users are responsible for verifying compliance with any applicable patents or licenses; the supplier assumes no liability for infringement or regulatory violations.

Packaging & Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	¥1,598.0	Spot (shipment within 5 days)
96 T	¥2,558.0	Spot (shipment within 5 days)

Research Background

Keratinocyte growth factor (KGF/FGF‑7) is a pivotal signaling molecule during the re‑epithelialization phase of wound healing, acting through the FGFR2b receptor to stimulate epithelial cell proliferation and differentiation. Inflammatory cytokines (IL‑1α, IL‑1β, IL‑6) up‑regulate fibroblast KGF expression, thereby promoting wound repair. This ELISA kit enables highly sensitive quantification of KGF in cell culture supernatants, serum, or plasma, facilitating investigation of its role in cellular communication, tissue regeneration, and pharmacological interventions.

Note: Information is subject to change with product updates; for the latest details, please contact Yakein.