猪游离三碘甲状腺原氨酸 ELISA定量试剂盒(fT3)

猪游离三碘甲状腺原氨酸 (fT3) ELISA定量试剂盒

货号：KTE0004-B10-1 | 产品类型：ELISA核心产品

产品概述

本试剂盒是一款基于竞争法ELISA原理的高灵敏度定量检测试剂盒，专门用于精准检测猪血清、血浆及其他生物液体样本中的游离三碘甲状腺原氨酸 (fT3) 浓度。作为EliKine™系列的核心产品，其设计旨在为甲状腺功能研究提供可靠、特异的分析工具。

核心特性与优势

• 高灵敏度与特异性：最低检测限达2.0 pmol/L，对待测物类似物无显著交叉反应或背景干扰，确保结果的准确性。
• 广泛的种属适用性：除猪外，经测试亦适用于人、小鼠、大鼠、山羊、绵羊、狗、兔子、牛、猴子、鸡等多种物种的研究。
• 优化的检测范围：提供2 pmol/L 至 32 pmol/L的宽动态检测范围，覆盖生理及病理状态下的典型浓度。
• 即用型完整组分：试剂盒包含进行实验所需的全部核心组分，如预包被微孔板、标准品、生物素化检测抗体及高纯度酶联物，开箱即用，简化操作流程。

检测背景信息

游离三碘甲状腺原氨酸 (fT3) 是循环血液中甲状腺激素的活性形式，其水平不受甲状腺素结合球蛋白 (TBG) 浓度的影响，因此能更直接、真实地反映甲状腺的功能状态，是评估甲状腺健康的关键指标之一。

试剂盒组分（详细列表）

• fT3 抗体预包被微孔板
• fT3 标准品
• 生物素偶联的fT3
• Avidin-HRP
• HRP底物A & HRP底物B
• 反应终止液
• 洗涤缓冲液
• 封板膜

技术参数

检测方法	竞争法ELISA	样本类型	血清、血浆、其它生物液体
检测范围	2 - 32 pmol/L	灵敏度	2.0 pmol/L
保存条件	未开封试剂盒请于2-8℃保存	运输条件	冰袋运输（蓝冰）

订购信息

规格	价格 (元)	货期
48 T	1598.0	期货（预计4个工作日内发货）
96 T	2558.0	期货（预计4个工作日内发货）

重要操作提示

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为获得最佳结果，建议对样品和标准品进行复孔（双孔或三孔）检测，并确保充分混匀。
• 实验过程中请使用一次性耗材，以避免交叉污染。

警告：本产品仅用于科学研究，不适用于人类或临床诊断。使用前请务必阅读完整说明书。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Porcine Free Triiodothyronine (fT3) ELISA Quantitative Kit

Catalog Number: KTE0004-B10-1 | Product Type: ELISA Core Product

Product Overview

This kit is a highly sensitive quantitative detection kit based on the competitive ELISA principle, specifically designed for the accurate quantification of free triiodothyronine (fT3) concentration in porcine serum, plasma, and other biological fluid samples. As a core product of the EliKine™ series, it is engineered to provide a reliable and specific analytical tool for thyroid function research.

Core Features & Advantages

• High Sensitivity & Specificity: The minimum detection limit reaches 2.0 pmol/L, with no significant cross-reactivity or background interference with analyte analogs, ensuring result accuracy.
• Broad Species Applicability: Besides porcine, it has been validated for research use in multiple species including human, mouse, rat, goat, sheep, dog, rabbit, bovine, monkey, chicken, and more.
• Optimized Detection Range: Provides a wide dynamic detection range of 2 pmol/L to 32 pmol/L, covering typical concentrations under physiological and pathological conditions.
• Ready-to-Use Complete Components: The kit includes all core components required for the assay, such as pre-coated microplate, standards, biotinylated detection antibody, and high-purity enzyme conjugate, ready for immediate use, simplifying the operational procedure.

Assay Background Information

Free triiodothyronine (fT3) is the active form of thyroid hormone in the circulation. Its level is not affected by thyroxine-binding globulin (TBG) concentration, thus providing a more direct and true reflection of thyroid functional status, making it a key indicator for assessing thyroid health.

Kit Components (Detailed List)

• fT3 Antibody Pre-coated Microplate
• fT3 Standard
• Biotin-conjugated fT3
• Avidin-HRP
• HRP Substrate A & HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Sealer

Technical Parameters

Assay Method	Competitive ELISA	Sample Type	Serum, Plasma, Other Biological Fluids
Detection Range	2 - 32 pmol/L	Sensitivity	2.0 pmol/L
Storage Condition	Store unopened kit at 2-8°C	Shipping Condition	Shipped with ice packs (blue ice)

Ordering Information

Specification	Price (RMB)	Lead Time
48 T	1598.0	Stock (Expected shipment within 4 business days)
96 T	2558.0	Stock (Expected shipment within 4 business days)

Important Operational Notes

• Do not mix reagents from different lot numbers or expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• For optimal results, it is recommended to test samples and standards in replicates (duplicate or triplicate) and ensure thorough mixing.
• Use disposable consumables during the experiment to avoid cross-contamination.

Warning: This product is for research use only, not intended for human or clinical diagnostic use. Please read the complete instructions before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific information, please consult Yaken Company.