MDA-7 (Melanoma Differentiatio

重组人白介素-24 (IL-24) 蛋白，His标签（无动物源）

货号：PRP1939

产品概述：

本品为高纯度、无动物源表达的重组人白介素-24 (IL-24) 蛋白，又称MDA-7 (黑色素瘤分化相关基因7蛋白)。该蛋白通过大肠杆菌表达系统生产，带有His标签，序列涵盖Gln52-Leu206，预测分子量为19.10 kDa，为细胞生物学研究提供可靠工具。

产品特点

• 高纯度：经SDS-PAGE验证，纯度超过95%，确保实验结果的可靠性与一致性。
• 极低内毒素：每微克蛋白内毒素含量<0.1 EU（LAL法测定），尽可能减少对细胞实验的干扰。
• 无动物源生产：在生产过程中完全避免动物源性成分，降低了外源因子污染的风险，适用于对来源敏感的研究应用。
• 经功能验证的活性：通过转染人IL-20Rα和IL-20Rβ的BaF3细胞化学吸引能力测定，其ED50值<120 pg/mL。

产品信息

背景信息

白细胞介素-24 (IL-24) 是IL-10细胞因子家族的成员，也被称为MDA-7。它通过IL-20R1/IL-20R2和IL-22R1/IL-20R2受体复合物传导信号，在伤口愈合、抗菌免疫反应及细胞分化等生理过程中扮演重要角色。

使用说明

复溶建议：

• 开盖前请先离心收集粉末。
• 推荐使用本品配套的复溶缓冲液进行复溶，初始浓度不低于100 µg/mL。
• 可根据后续实验需求，使用含载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液进行进一步稀释。

保存与运输：

• 保存：冻干粉在-20°C下可稳定保存1年。复溶后于2-8°C下可保存1周。稀释分装后，在-20°C或-80°C下可保存3-6个月。请避免反复冻融。
• 运输：全程采用冰袋（蓝冰）运输，以确保产品稳定性。

规格与价格

警告：本品仅供研究使用，不适用于人类或临床诊断。使用本品可能涉及现有专利，使用者有责任确保其应用不侵犯第三方知识产权。

别名：MDA-7, IL-24, IL 24, IL24, Melanoma Differentiation-Associated gene 7 protein

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-24 (IL-24) Protein, His Tag (Animal-Free)

Catalog Number: PRP1939

Product Overview:

This product is a high-purity, animal-free expressed recombinant human Interleukin-24 (IL-24) protein, also known as MDA-7 (Melanoma Differentiation-Associated gene 7 protein). This protein is produced in an E. coli expression system with a His tag. The sequence covers Gln52-Leu206, with a predicted molecular weight of 19.10 kDa, providing a reliable tool for cell biology research.

Features

• High Purity: Verified by SDS-PAGE, purity >95%, ensuring reliable and consistent experimental results.
• Ultra-Low Endotoxin: Endotoxin level <0.1 EU per µg protein (determined by LAL method), minimizing interference in cell-based assays.
• Animal-Free Production: The production process completely avoids animal-derived components, reducing the risk of contamination by adventitious agents, making it suitable for source-sensitive research applications.
• Functionally Validated Activity: Determined by its chemotactic ability using BaF3 cells transfected with human IL-20Rα and IL-20Rβ, with an ED₅₀ <120 pg/mL.

Product Information

Background

Interleukin-24 (IL-24) is a member of the IL-10 cytokine family, also known as MDA-7. It signals through the IL-20R1/IL-20R2 and IL-22R1/IL-20R2 receptor complexes, playing important roles in physiological processes such as wound healing, antibacterial immune responses, and cell differentiation.

Usage

Reconstitution Recommendation:

• Centrifuge the vial briefly before opening to collect the powder at the bottom.
• It is recommended to reconstitute the lyophilized protein using the sterile reconstitution buffer provided with this product to a concentration no less than 100 µg/mL.
• For further dilution, carrier proteins (e.g., 0.1% BSA, 10% FBS, 5% HSA) can be added to the buffer according to experimental needs.

Storage & Shipping:

• Storage: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, it can be stored at 2-8°C for up to 1 week. For long-term storage, aliquot and store at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.
• Shipping: Shipped with blue ice packs to ensure product stability.

Specifications & Pricing

Warning: This product is for research use only, not intended for human or diagnostic use. The use of this product may involve existing patents. It is the responsibility of the user to ensure that their application does not infringe upon third-party intellectual property rights.

Alias: MDA-7, IL-24, IL 24, IL24, Melanoma Differentiation-Associated gene 7 protein

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Ya Keyin Biotech for specific details.