Macrophage Colony-Stimulating

重组人巨噬细胞集落刺激因子（M-CSF）—— 专为细胞培养研究设计的高纯度细胞因子

产品概览

产品名称：重组人巨噬细胞集落刺激因子（M-CSF）
英文名称：Human M-CSF Protein
货号：PRP1937
种属来源：人源（Homo sapiens）
蛋白序列：氨基酸残基 Glu33–Ser190（UniProt ID: P09603-3）
表达系统：大肠杆菌（E. coli）原核表达
分子量：约 18.5 kDa
纯度：>95%（SDS-PAGE 检测）
标签状态：无标签（避免干扰生物学活性或下游应用）
产品形式：冻干粉，冻干缓冲液：20 mM Tris, 150 mM NaCl, pH 8.0（无菌）

生物活性与功能特性

本品通过其诱导 M-NFS-60 细胞增殖的能力进行功能性验证，实测 ED₅₀ 为 0.7344 ng/mL，表明其在低浓度下即具备显著的生物学效应，适用于需高灵敏度检测的细胞因子响应实验。M-CSF（CSF-1）作为关键的造血调控因子，主要由淋巴细胞、单核细胞、成纤维细胞、内皮细胞、成肌细胞及成骨细胞等多种细胞类型分泌，是调控单核/巨噬细胞系增殖、分化与存活的核心因子，广泛应用于体外巨噬细胞模型构建、免疫微环境研究及肿瘤微环境相关实验。

质量控制与纯度保障

• 内毒素水平：通过 LAL 法检测，每微克蛋白中内毒素含量 < 0.1 EU，满足多数细胞培养系统对低内毒素环境的严苛要求。
• 纯度验证：采用 SDS-PAGE 进行质量监控，确保蛋白条带单一，无明显降解或杂蛋白污染。
• 无标签设计：未引入 His、GST 等亲和标签，尽可能保留天然构象与受体结合能力，适用于需要避免标签干扰的信号通路研究。

使用建议与操作规范

复溶指导：

开盖前请短暂离心（约 10,000 × g，1 分钟）以确保冻干粉完全沉于管底。推荐使用配套的 Reconstitution Buffer 进行复溶，初始浓度不应低于 100 µg/mL，以维持蛋白稳定性。复溶后可根据实验需求，使用含载体蛋白（如 0.1% BSA、5% HSA 或 10% FBS）的缓冲液进一步稀释，以减少吸附损失。

保存与稳定性：

• 冻干状态：–20°C 下可稳定保存 12 个月。
• 复溶后（未稀释）：2–8°C 可保存不超过 7 天。
• 稀释后分装：建议分装后于 –20°C 或 –80°C 保存，可稳定 3–6 个月。请避免反复冻融，以维持蛋白活性。

运输条件：

采用冰袋（蓝冰）运输，确保产品在运输过程中维持低温状态，保障冻干结构完整性。

规格与订购信息

重要声明

本产品仅限于科学研究用途，不适用于人类或临床诊断。所有推荐应用基于文献与实验数据，不代表对任何专利或知识产权的授权。使用者应自行确认其研究行为是否符合相关法律法规及专利许可要求。供应商不对因使用本产品而引发的专利侵权或其他法律纠纷承担责任。

背景与参考信息

M-CSF（Macrophage Colony-Stimulating Factor 1, CSF1）是调控髓系细胞发育的关键细胞因子，其基因 ID 为 1435，UniProt 编号为 P09603-3。完整序列信息可参考：UniProt P09603-3。其在体外常用于诱导单核细胞向巨噬细胞分化，构建炎症、肿瘤或组织修复模型。

常见别名（用于数据库检索）

Macrophage Colony-Stimulating Factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Macrophage Colony-Stimulating Factor (M-CSF) – A High-Purity Cytokine Designed for Cell Culture Research

Product Overview

Product Name: Recombinant Human Macrophage Colony-Stimulating Factor (M-CSF)
English Name: Human M-CSF Protein
Catalog Number: PRP1937
Species Source: Human (Homo sapiens)
Protein Sequence: Amino acid residues Glu33–Ser190 (UniProt ID: P09603-3)
Expression System: E. coli prokaryotic expression
Molecular Weight: Approximately 18.5 kDa
Purity: >95% (as determined by SDS-PAGE)
Tag Status: Tag-free (to avoid interference with biological activity or downstream applications)
Product Form: Lyophilized powder, Lyophilization Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0 (sterile)

Biological Activity and Functional Characteristics

This product is functionally validated by its ability to induce M-NFS-60 cell proliferation, with a measured ED₅₀ of 0.7344 ng/mL, indicating significant biological effects at low concentrations, making it suitable for cytokine response experiments requiring high sensitivity. M-CSF (CSF-1), as a key hematopoietic regulatory factor, is primarily secreted by various cell types including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts, and osteoblasts. It is a core factor regulating the proliferation, differentiation, and survival of the monocyte/macrophage lineage, widely used in constructing in vitro macrophage models, studying the immune microenvironment, and experiments related to the tumor microenvironment.

Quality Control and Purity Assurance

• Endotoxin Level: Determined by LAL method, endotoxin content is < 0.1 EU per µg of protein, meeting the stringent requirements of most cell culture systems for a low endotoxin environment.
• Purity Verification: Monitored by SDS-PAGE to ensure a single protein band with no significant degradation or contaminating proteins.
• Tag-Free Design: No affinity tags such as His or GST are introduced, maximizing the preservation of native conformation and receptor-binding capability, suitable for signal pathway studies requiring avoidance of tag interference.

Usage Recommendations and Handling Guidelines

Reconstitution Guidance:

Briefly centrifuge the vial (approx. 10,000 × g, 1 minute) before opening to ensure the lyophilized powder is completely at the bottom of the tube. Reconstitution using the provided Reconstitution Buffer is recommended; the initial concentration should not be less than 100 µg/mL to maintain protein stability. After reconstitution, it can be further diluted using buffers containing carrier proteins (e.g., 0.1% BSA, 5% HSA, or 10% FBS) as required by the experiment, to reduce adsorption losses.

Storage and Stability:

• Lyophilized State: Stable for 12 months when stored at –20°C.
• After Reconstitution (undiluted): Can be stored at 2–8°C for up to 7 days.
• After Dilution and Aliquoting: Recommended to aliquot and store at –20°C or –80°C, stable for 3–6 months. Avoid repeated freeze-thaw cycles to maintain protein activity.

Shipping Conditions:

Shipped with ice packs (blue ice) to ensure the product remains cold during transit, preserving the integrity of the lyophilized formulation.

Specifications and Ordering Information

Important Notice

This product is intended for research use only, not for human or clinical diagnostic use. All recommended applications are based on literature and experimental data and do not constitute authorization for any patent or intellectual property rights. Users are responsible for ensuring their research activities comply with relevant laws, regulations, and patent licensing requirements. The supplier is not liable for patent infringement or other legal disputes arising from the use of this product.

Background and Reference Information

M-CSF (Macrophage Colony-Stimulating Factor 1, CSF1) is a key cytokine regulating myeloid cell development. Its gene ID is 1435, and UniProt ID is P09603-3. Complete sequence information can be found at: UniProt P09603-3. It is commonly used in vitro to induce monocyte differentiation into macrophages, and for modeling inflammation, cancer, or tissue repair.

Common Aliases (for database searches)

Macrophage Colony-Stimulating Factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please contact Yakeyin Company.