Granulocyte colony-stimulating

重组小鼠粒细胞集落刺激因子 (G-CSF)，His 标签

货号：PRP1100
产品类型：重组细胞因子蛋白
种属来源：小鼠 (Mus musculus)
表达宿主：大肠杆菌表达系统

产品描述

本品为高纯度重组小鼠 Granulocyte colony-stimulating factor (G-CSF) 蛋白，在其C末端带有 His 标签，采用大肠杆菌系统表达并纯化。该重组蛋白的氨基酸序列对应于小鼠G-CSF的Val31-Ala208片段（UniProt编号：P09920），分子量约为19.8 kDa。产品经SDS-PAGE验证纯度高于98%，并采用LAL法检测内毒素，每微克蛋白中内毒素含量低于0.1 EU，适用于对纯度及低内毒素有严格要求的细胞培养与功能研究。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 > 98%
• 低内毒素：内毒素含量 < 0.1 EU/µg（LAL法测定）
• 生物活性：经M-NFS-60细胞增殖试验验证，其ED₅₀值为1.916 ng/mL
• 物理形态：冻干粉形式，缓冲体系为20 mM Tris, 150 mM NaCl, 5% Sucrose, 5% Mannitol, pH 9.0
• 标签类型：C端His标签，便于识别与后续纯化

应用方向

• 造血干细胞与祖细胞体外增殖与分化研究
• 粒细胞生成及相关信号通路机制探讨
• 细胞因子功能与受体互作分析
• 药物筛选与药效学评估模型构建

使用建议

使用前请轻微离心，确保样品聚集于管底。推荐使用原厂提供的复溶缓冲液（Reconstitution Buffer）进行复溶，建议复溶浓度不低于100 µg/mL，复溶后可依实验需求进一步稀释。为维持蛋白稳定性，建议在含有载体蛋白（如0.1% BSA或HSA）的缓冲液中进行稀释与分装。

保存与运输

保存条件：冻干粉可在-20°C条件下稳定保存1年；复溶后置于2–8°C可保存1周，稀释后分装保存于-20°C或-80°C可维持3–6个月活性，请避免反复冻融。
运输方式：全程蓝冰低温运输，保障产品稳定性。

规格与价格

注意事项

本产品仅限于科研用途，不适用于人体或临床诊断。使用者应负责确保其应用不侵犯现有专利或许可协议。

基因ID：12985
别名：Csf3, G-CSF, Granulocyte colony-stimulating factor
UniProt 编号：P09920

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Murine Granulocyte Colony-Stimulating Factor (G‑CSF), His‑Tag

Catalog No.：PRP1100
Product Type：Recombinant cytokine protein
Species：Mouse (Mus musculus)
Expression Host：E. coli expression system

Product Description

This product is a high‑purity recombinant mouse Granulocyte colony‑stimulating factor (G‑CSF) protein bearing a C‑terminal His tag, expressed in an E. coli system and purified. The amino‑acid sequence corresponds to the mouse G‑CSF Val31‑Ala208 fragment (UniProt ID: P09920) with an approximate molecular weight of 19.8 kDa. Purity > 98% was confirmed by SDS‑PAGE, and endotoxin levels were measured by LAL assay at < 0.1 EU/µg protein, making it suitable for cell culture and functional studies requiring high purity and low endotoxin.

Key Attributes

• High Purity：> 98% by SDS‑PAGE
• Low Endotoxin：< 0.1 EU/µg (LAL assay)
• Biological Activity：ED₅₀ = 1.916 ng/mL in M‑NFS‑60 cell proliferation assay
• Physical Form：Lyophilized powder; formulation: 20 mM Tris, 150 mM NaCl, 5 % sucrose, 5 % mannitol, pH 9.0
• Tag Type：C‑terminal His tag for detection and downstream purification

Applications

• In vitro expansion and differentiation of hematopoietic stem and progenitor cells
• Granulopoiesis and associated signaling pathway studies
• Cytokine function and receptor interaction analysis
• Drug screening and pharmacodynamic model development

Recommended Use

Prior to use, briefly centrifuge to collect the sample at the bottom of the tube. Reconstitute with the provided Reconstitution Buffer; a concentration of ≥ 100 µg/mL is recommended. The solution can be further diluted according to experimental needs. To maintain protein stability, dilute and aliquot in a buffer containing a carrier protein (e.g., 0.1 % BSA or HSA).

Storage and Shipping

Storage Conditions：Lyophilized powder is stable at ‑20 °C for up to 1 year; once reconstituted, store at 2–8 °C for up to 1 week. After dilution, aliquots stored at ‑20 °C or ‑80 °C retain activity for 3–6 months. Avoid repeated freeze‑thaw cycles.
Shipping Method：Full‑scale blue‑ice temperature‑controlled transport to ensure product stability.

Specifications and Pricing

Precautions

This product is for research use only and is not intended for human or clinical diagnostic applications. Users are responsible for ensuring that their use does not infringe existing patents or licensing agreements.

Gene ID：12985
Synonyms：Csf3, G‑CSF, Granulocyte colony‑stimulating factor
UniProt ID：P09920

Note: The information above may change due to product optimization or upgrades; please contact Yakeyin Corp. for the most current details.