soluble CD40 Ligand, TNFSF5, T

产品概述

Human CD40L Protein, His tag (Animal-Free) 是一种高纯度、无动物源的重组蛋白，专为细胞培养及相关研究应用设计。该产品通过大肠杆菌表达系统生产，具有卓越的生物活性和低内毒素特性，适用于对细胞因子功能研究要求严格的实验环境。

产品详情

• 货号: PRP1920
• 产品类型: 细胞因子
• 种属来源: 人源
• 表达宿主: 大肠杆菌
• 氨基酸序列: Met113-Leu261（人CD40配体）
• 分子量: 约 16 kDa
• 纯度: >95%（SDS-PAGE检测）
• 生物活性: 经功能验证，可诱导人外周血单个核细胞分泌IL-8，ED₅₀值＜5 ng/mL
• 内毒素水平: ＜0.1 EU/µg（LAL法测定）
• 产品形态: 冻干粉（冻干缓冲液为无菌PBS, pH 8.0）

应用特点

本品适用于T细胞与抗原呈递细胞（如B细胞、巨噬细胞）相互作用的研究，尤其在免疫应答、B细胞活化与抗体产生机制研究中具有重要应用价值。其无动物源特性进一步降低了引入外源因子的风险，提高了实验结果的可靠性。

使用说明

• 复溶建议: 推荐使用配套复溶缓冲液，复溶浓度不低于100 µg/mL。
• 保存条件: 冻干粉可在-20°C稳定保存1年；复溶后于2-8°C可保存1周。
• 长期保存: 在含有载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液中稀释后，可分装存于-20°C或-80°C，避免反复冻融。

规格与价格

背景信息

人CD40配体（CD40L，亦称CD154）是肿瘤坏死因子超家族（TNFSF5）成员之一，作为一种关键的免疫调节细胞因子，在T细胞依赖性B细胞活化、抗体类别转换及免疫记忆形成中发挥核心作用。其与CD40受体结合后可激活多种信号通路，影响免疫细胞增殖、分化与存活。

注意事项

使用前请离心处理。本品仅供研究使用，不应用于人体或临床诊断。用户应确保其应用不违反相关专利或许可规定。

运输条件

本品采用冰袋（蓝冰）运输，以保障蛋白稳定性。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Human CD40L Protein, His tag (Animal-Free) is a high‑purity, animal‑origin‑free recombinant protein designed specifically for cell culture and related research applications. The product is produced using an Escherichia coli expression system, exhibiting excellent biological activity and low endotoxin levels, and is suitable for experimental settings that demand stringent cytokine functional studies.

Product Details

• Catalog No.: PRP1920
• Product Type: Cytokine
• Species Origin: Human
• Expression Host: Escherichia coli
• Amino‑acid Sequence: Met113‑Leu261 (human CD40 ligand)
• Molecular Weight: ~16 kDa
• Purity: >95 % (SDS‑PAGE analysis)
• Biological Activity: Functionally validated; induces IL‑8 secretion from human peripheral blood mononuclear cells (PBMCs) with an EC₅₀ < 5 ng/mL
• Endotoxin Level: < 0.1 EU/µg (LAL assay)
• Product Form: Lyophilized powder (lyophilization buffer: sterile PBS, pH 8.0)

Application Highlights

This product is suitable for studies of interactions between T cells and antigen‑presenting cells (e.g., B cells, macrophages), and is particularly valuable in investigations of immune responses, B‑cell activation, and antibody generation mechanisms. Its animal‑origin‑free nature further reduces the risk of introducing exogenous factors, enhancing the reliability of experimental outcomes.

Usage Instructions

• Reconstitution Recommendation: Use the provided reconstitution buffer; reconstitute to a concentration of ≥100 µg/mL.
• Storage Conditions: Lyophilized powder is stable at –20 °C for up to 1 year; after reconstitution, store at 2–8 °C for up to 1 week.
• Long‑term Storage: After dilution in a buffer containing carrier protein (e.g., 0.1 % BSA, 10 % FBS, or 5 % HSA), aliquot and store at –20 °C or –80 °C, avoiding repeated freeze–thaw cycles.

Specifications & Pricing

Background Information

Human CD40 ligand (CD40L, also known as CD154) is a member of the tumor necrosis factor superfamily (TNFSF5) and functions as a pivotal immunoregulatory cytokine. It plays a central role in T‑cell‑dependent B‑cell activation, antibody class switching, and the formation of immune memory. Binding to the CD40 receptor triggers multiple signaling pathways, influencing immune cell proliferation, differentiation, and survival.

Precautions

Prior to use, centrifuge the solution. This product is for research purposes only and must not be used in humans or clinical diagnostics. Users should ensure that their applications do not infringe any patents or licensing agreements.

Shipping Conditions

The product is shipped with gel ice packs (blue ice) to maintain protein stability.

Note: The above information may change due to product optimization or upgrades; for the most current details, please contact Yakein Company.