FGFA, Keratinocyte Growth Fact

重组人成纤维细胞生长因子10（FGF-10），His标签（无动物源）

货号：PRP1913

产品类型：重组细胞因子

种属来源：人

产品概述

本品为一种高纯度、无动物源成分的重组人FGF-10蛋白，携带His标签，适用于各类细胞培养及功能研究。该蛋白经大肠杆菌表达系统制备，具备优异的生物活性与批次稳定性，可广泛应用于上皮细胞增殖、分化研究及相关信号通路分析。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 >98%
• 低内毒素：LAL法检测内毒素含量 <0.1 EU/μg
• 高生物活性：比活性 >1.2×10⁵ IU/mg，ED₅₀ <8 ng/mL（基于3T3细胞增殖实验）
• 无动物源表达：生产过程无动物成分，降低外源因子风险

技术参数

• 表达宿主：大肠杆菌
• 蛋白长度：169个氨基酸（Leu40-Ser208）
• 分子量：约19 kDa
• 标签类型：His标签
• 蛋白形态：冻干粉（冻干缓冲液：无菌PBS，pH 7.4）

应用建议

本品适用于细胞增殖、分化研究，尤其在上皮细胞和角质形成细胞相关实验中表现优异。推荐复溶浓度不低于100 μg/mL，复溶后可依据实验需求进一步稀释。

保存与运输

• 保存条件：冻干粉-20℃可保存1年；复溶后2-8℃可保存1周；添加载体蛋白（如0.1% BSA）后分装保存于-20℃或-80℃，可稳定3-6个月
• 运输条件：冰袋运输（蓝冰）

注意事项

• 使用前请离心，避免粉末损失
• 建议使用配套复溶缓冲液进行复溶
• 避免反复冻融以维持蛋白活性
• 本品仅供科研使用，不适用于临床诊断或人体应用

背景信息

FGF-10是一种多功能的间充质-上皮信号传导因子，在胚胎发育、组织修复及细胞调控中发挥重要作用。其异常表达与多种癌症及遗传性疾病相关，是研究上皮-间质相互作用的重要分子工具。

产品规格与价格

规格	价格（元）	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	3289	请咨询
1 mg	请咨询

警告：本产品仅用于科研用途，不适用于人体或临床诊断。使用者应确保应用方式不违反相关专利或许可协议。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Fibroblast Growth Factor-10 (FGF-10), His Tag (Animal-Free)

Catalog Number: PRP1913

Product Type: Recombinant Cytokine

Species: Human

Product Overview

This product is a high-purity, animal-free recombinant human FGF-10 protein carrying a His tag, suitable for various cell culture and functional studies. This protein is produced using an E. coli expression system, exhibits excellent biological activity and batch-to-batch consistency, and can be widely applied in studies of epithelial cell proliferation, differentiation, and related signaling pathway analysis.

Key Features

• High Purity: Purity >98% as determined by SDS-PAGE.
• Low Endotoxin: Endotoxin level <0.1 EU/μg, measured by LAL assay.
• High Biological Activity: Specific activity >1.2×10⁵ IU/mg, ED₅₀ <8 ng/mL (based on a 3T3 cell proliferation assay).
• Animal-Free Expression: Production process is free of animal-derived components, reducing the risk of adventitious agents.

Technical Parameters

• Expression Host: E. coli
• Protein Length: 169 amino acids (Leu40-Ser208)
• Molecular Weight: Approximately 19 kDa
• Tag Type: His Tag
• Form: Lyophilized powder (Lyophilized from sterile PBS, pH 7.4)

Application Suggestions

This product is suitable for cell proliferation and differentiation studies, particularly excelling in experiments related to epithelial cells and keratinocytes. The recommended reconstitution concentration is not less than 100 μg/mL. After reconstitution, it can be further diluted according to experimental needs.

Storage and Shipping

• Storage: Lyophilized powder can be stored at -20°C for 1 year. After reconstitution, store at 2-8°C for up to 1 week. For longer storage, aliquot after adding a carrier protein (e.g., 0.1% BSA) and store at -20°C or -80°C for 3-6 months.
• Shipping: Shipped with ice packs (blue ice).

Precautions

• Centrifuge the vial briefly before use to avoid powder loss.
• It is recommended to reconstitute using the provided reconstitution buffer.
• Avoid repeated freeze-thaw cycles to maintain protein activity.
• This product is for research use only. Not intended for diagnostic or therapeutic use in humans.

Background

FGF-10 is a multifunctional mesenchymal-epithelial signaling factor that plays important roles in embryonic development, tissue repair, and cellular regulation. Its aberrant expression is associated with various cancers and genetic diseases, making it an important molecular tool for studying epithelial-mesenchymal interactions.

Specifications and Pricing

Specification	Price (RMB)	Availability
5 μg	588	In Stock (Ships Next Day)
20 μg	1498	In Stock (Ships Next Day)
100 μg	3289	Please Inquire
1 mg	Please Inquire

Warning: This product is for research use only. Not for use in humans or for diagnostic procedures. Users should ensure their application does not infringe relevant patents or license agreements.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Ya Keyin Company for specific details.