CXCL16 重组小鼠CXC趋化因子配体16 小鼠重组蛋白

Mouse CXCL16 Protein, His tag (Animal-Free) - PRP1121

一款高纯度、高活性、动物源无添加的重组小鼠CXC趋化因子配体16，专为严谨的细胞培养研究而设计。

产品概述

Mouse CXCL16 Protein (货号: PRP1121) 是通过大肠杆菌表达系统重组表达的小鼠源CXCL16蛋白，其氨基酸序列来源于小鼠源CXCL16 (Asn 27-Pro 114)。本品以His标签进行标记，最终以冻干粉形式提供，纯度经SDS-PAGE检测大于98%，确保了批次间的稳定性和实验结果的可靠性。CXCL16属于CXC趋化因子家族，在介导先天性免疫反应中，特别是对表达CXCR6受体的T细胞和NKT细胞具有趋化作用。

关键特性与优势

• 高生物活性：经功能验证，本品能够有效趋化转染了小鼠CXCR6的BaF3细胞，其半数有效浓度（ED₅₀）< 3 ng/mL。
• 超高纯度：SDS-PAGE检测纯度 > 98%，有效减少杂质对实验的干扰。
• 极低内毒素：通过LAL法测定，每微克蛋白内毒素含量 < 0.1 EU，适合对内毒素敏感的实验体系。
• 动物源无添加：整个生产过程不添加任何动物源成分，降低了病毒和外来因子污染的风险。
• 明确的分子特性：由88个氨基酸组成，预测分子量为10.74 kDa。

产品规格

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	请咨询
100 μg	3469	请咨询
1 mg	请咨询	请咨询

产品形式与保存

本品为冻干粉，冻干缓冲液为无菌PBS，pH 7.4。

• 保存建议：冻干粉应在-20°C条件下保存，有效期1年。复溶后于2-8°C可保存1周。若在含有载体蛋白（如0.1% BSA）的缓冲液中进一步稀释并分装，建议在-20°C或-80°C下保存3-6个月，并避免反复冻融。

使用说明

• 复溶：开盖前请先离心。推荐使用产品配套的复溶缓冲液进行复溶，建议复溶浓度不低于100 µg/mL。用户可根据后续实验需求进行稀释。
• 运输条件：本品采用冰袋（蓝冰）运输。

背景信息

CXCL16（C-X-C motif chemokine 16），也被称为SRPSOX，是一种分子量约为9.8 kDa的趋化因子。它能够被TNF-α、IL-1和IFN-γ等炎性因子诱导表达，并通过与其受体CXCR6结合，在免疫细胞（如T细胞、NKT细胞）的趋化、宿主抵抗革兰氏阳性及阴性细菌的防御机制中发挥关键作用。其UniProt登录号为Q8BSU2。

注意事项

警告：本产品仅用于科学研究用途，不适用于人类或临床诊断。使用本产品不应违反任何专利或许可证规定，对于因此可能产生的任何专利侵权或其他违规行为，我方不承担任何责任。

别名参考：CXCL16, CXCL-16, CXCL 16, C-X-C motif chemokine 16, SRPSOX

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse CXCL16 Protein, His tag (Animal-Free) - PRP1121

A high-purity, high-activity, animal-origin‑free recombinant mouse CXC chemokine ligand 16, specifically designed for rigorous cell culture studies.

Product Overview

Mouse CXCL16 Protein (catalog number: PRP1121) is a recombinant mouse‑derived CXCL16 expressed via an Escherichia coli system; its amino‑acid sequence corresponds to mouse CXCL16 (Asn 27‑Pro 114). The product is tagged with a His tag and supplied as a lyophilized powder. SDS‑PAGE analysis confirms purity > 98%, ensuring batch‑to‑batch consistency and reliable experimental results. CXCL16 belongs to the CXC chemokine family and mediates innate immune responses, notably chemotaxis of T cells and NKT cells expressing the CXCR6 receptor.

Key Features and Advantages

• High biological activity: Functionally validated to efficiently chemotax BaF3 cells transfected with mouse CXCR6, with a half‑maximal effective concentration (ED₅₀) < 3 ng/mL.
• Ultra‑high purity: SDS‑PAGE purity > 98%, effectively minimizing impurity interference in assays.
• Extremely low endotoxin: Determined by LAL assay, endotoxin level < 0.1 EU per µg protein, suitable for endotoxin‑sensitive systems.
• Animal‑origin free: No animal‑derived components are added throughout production, reducing the risk of viral or foreign‑agent contamination.
• Well‑defined molecular characteristics: Composed of 88 amino acids with a predicted molecular weight of 10.74 kDa.

Product Specifications

Specification	Price (CNY)	Lead Time
5 μg	588	In stock (next‑day shipment)
20 μg	1498	Please inquire
100 μg	3469	Please inquire
1 mg	Please inquire	Please inquire

Product Formulation and Storage

The product is supplied as a lyophilized powder with a sterile PBS (pH 7.4) reconstitution buffer.

• Storage recommendation: Lyophilized powder should be stored at –20 °C, shelf‑life 1 year. After reconstitution, store at 2‑8 °C for up to 1 week. If further diluted into a buffer containing carrier protein (e.g., 0.1 % BSA) and aliquoted, store at –20 °C or –80 °C for 3‑6 months, and avoid repeated freeze‑thaw cycles.

Usage Instructions

• Reconstitution: Centrifuge the vial prior to opening. It is recommended to reconstitute with the supplied buffer, achieving a final concentration of at least 100 µg/mL. Users may dilute further according to experimental requirements.
• Shipping condition: Shipped with ice packs (blue ice).

Background Information

CXCL16 (C‑X‑C motif chemokine 16), also known as SRPSOX, is a chemokine with an approximate molecular weight of 9.8 kDa. Its expression is induced by inflammatory cytokines such as TNF‑α, IL‑1, and IFN‑γ. By binding to its receptor CXCR6, CXCL16 mediates chemotaxis of immune cells (e.g., T cells, NKT cells) and plays a critical role in host defense against Gram‑positive and Gram‑negative bacterial infections. Its UniProt accession number is Q8BSU2.

Precautions

Warning: This product is for scientific research use only and is not intended for human or clinical diagnostic applications. Use of this product must not violate any patents or licenses; we assume no responsibility for any patent infringement or other violations that may arise.

Alias references: CXCL16, CXCL-16, CXCL 16, C‑X‑C motif chemokine 16, SRPSOX

Note: The above information may change due to product optimization or upgrades; please consult Yakeyin Company for the latest details.