4-1BB ligand, CD137L, TNLG5A,

产品概览

产品名称：重组人4-1BB配体（His‑标签，Animal‑Free）
英文名称：Human 4‑1BBL Protein, His tag (Animal‑Free)
货号：PRP1217
别名：4‑1BB ligand, CD137L, TNLG5A, TNFSF9, Tumor necrosis factor ligand superfamily member 9
基因ID：8744
蛋白质ID：P41273

核心技术参数

• 氨基酸序列来源：人源4‑1BBL（Arg71‑Glu254），共184个残基，预测分子量约20.4 kDa。
• 表达宿主：大肠杆菌（无动物源）
• 标签类型：His‑tag
• 纯度：>95 %（SDS‑PAGE检测）
• 内毒素水平：<0.05 EU/µg（LAL法）
• 活性表征：在人体外周血单个核细胞（PBMC）中诱导IL‑8分泌，ED₅₀ 1‑5 ng/mL。

产品形态

冻干粉，包装于无菌PBS（pH 7.4）缓冲液中。每支产品均提供配套的复溶缓冲液，推荐复溶浓度≥100 µg/mL。

应用领域

本产品定位于细胞类核心产品，适用于细胞培养中的免疫共刺激研究，尤其是：

• 评估4‑1BB/4‑1BBL信号通路在抗原递呈细胞（B细胞、巨噬细胞、树突细胞）中的功能。
• 探讨T细胞（CD4⁺、CD8⁺）增殖与细胞因子分泌（NFκB、c‑Jun、p38）对免疫治疗的潜在影响。
• 用于体外免疫检查点模型、共刺激激活实验以及癌症免疫治疗前沿研究。

技术规格

使用说明

• 开盖后请先离心除去潜在颗粒。
• 建议使用本公司提供的复溶缓冲液进行溶解，确保浓度不低于100 µg/mL。
• 复溶后产品可在2 °C ~ 8 °C下保存最多1周；若加入载体蛋白或稳定剂（如0.1 % BSA、10 % FBS、5 % HSA），一次分装后可在‑20 °C或‑80 °C保存3–6个月。
• 避免反复冻融循环，以维持活性。

储存与运输

• 冻干粉：‑20 °C，保质期1年。
• 复溶液：2 °C ~ 8 °C，最长1周。
• 运输方式：冰袋（蓝冰）冷链运输，确保到货时仍为冻干状态。

安全与合规

本产品为研究用途（R&D）专用，**不适用于人类或临床诊断**。使用本产品的任何专利或许可证相关行为均需使用者自行负责，本公司不承担相关侵权或违规责任。

技术支持

如需进一步的实验方案、缓冲体系建议或批量定制，请联系您的商务代表或技术服务团队。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Recombinant Human 4-1BB Ligand (His‑Tag, Animal‑Free)
English Name: Human 4‑1BBL Protein, His tag (Animal‑Free)
Catalog Number: PRP1217
Synonyms: 4‑1BB ligand, CD137L, TNLG5A, TNFSF9, Tumor necrosis factor ligand superfamily member 9
Gene ID: 8744
Protein ID: P41273

Core Technical Parameters

• Amino Acid Sequence Source: Human 4‑1BBL (Arg71‑Glu254), 184 residues total, predicted molecular weight approximately 20.4 kDa.
• Expression Host: E. coli (Animal-Free)
• Tag Type: His‑tag
• Purity: >95 % (by SDS-PAGE)
• Endotoxin Level: <0.05 EU/µg (LAL method)
• Activity Characterization: Induces IL‑8 secretion in human peripheral blood mononuclear cells (PBMCs) with an ED₅₀ of 1‑5 ng/mL.

Product Form

Lyophilized powder, packaged in sterile PBS (pH 7.4) buffer. Each vial includes a corresponding reconstitution buffer; recommended reconstitution concentration is ≥100 µg/mL.

Application Areas

This product is positioned as a core cell biology product, suitable for immune co-stimulation studies in cell culture, particularly for:

• Evaluating the function of the 4‑1BB/4‑1BBL signaling pathway in antigen-presenting cells (B cells, macrophages, dendritic cells).
• Investigating the potential impact of T cell (CD4⁺, CD8⁺) proliferation and cytokine secretion (NFκB, c‑Jun, p38) on immunotherapy.
• Use in in vitro immune checkpoint models, co-stimulation activation assays, and cutting-edge cancer immunotherapy research.

Technical Specifications

Usage Instructions

• After opening, briefly centrifuge the vial to pellet any potential particulates.
• It is recommended to reconstitute using the provided reconstitution buffer to a final concentration ≥100 µg/mL.
• After reconstitution, the product can be stored at 2 °C ~ 8 °C for up to 1 week; if a carrier protein or stabilizer is added (e.g., 0.1 % BSA, 10 % FBS, 5 % HSA), aliquots can be stored at ‑20 °C or ‑80 °C for 3–6 months.
• Avoid repeated freeze-thaw cycles to maintain activity.

Storage and Shipping

• Lyophilized Powder: ‑20 °C, shelf life 1 year.
• Reconstituted Solution: 2 °C ~ 8 °C, up to 1 week.
• Shipping Method: Shipped on ice packs (blue ice) via cold chain to ensure arrival in lyophilized state.

Safety and Compliance

This product is intended for Research Use Only (RUO), **not for human or clinical diagnostic use**. The user is solely responsible for any actions related to patents or licenses concerning the use of this product; the company assumes no liability for infringement or non-compliance.

Technical Support

For further experimental protocols, buffer system recommendations, or bulk customization, please contact your sales representative or technical service team.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.