bone morphogenetic protein 9,

重组人BMP-9-His蛋白（大肠杆菌无动物源工艺）

货号：PRP1200 | 纯度：>98 % SDS-PAGE | 标签：His

Suitable for osteogenic induction, chondrogenic commitment and general SMAD-signaling studies in vitro.

产品速写

• 覆盖区间：Ser320-Arg429，人源天然序列（Q9UK05）
• 理论分子量：12.89 kDa（110 aa），实际SDS-PAGE表观条带与预期吻合
• 活性校定：ATDC5细胞碱性磷酸酶诱导实验，ED₅₀ ＜0.4 ng/mL
• 内毒素：LAL法检测，≤0.01 EU/µg，满足高敏感细胞实验需求
• 形态：冻干粉（20 mM sodium citrate, 0.2 M NaCl, pH 3.5），无需额外透析即可复溶
• 宿主平台：大肠杆菌表达体系，无动物源成分，降低外源病毒风险

典型应用方向

• 干细胞成骨诱导：与BMP-2/-7等组合，加速hMSC向成骨谱系定向分化
• 软骨细胞表型维持：用于ATDC5或小鼠原代软骨细胞ALP活性监测
• SMAD信号动力学研究：激活Smad1/5/8磷酸化的时序-剂量模型构建
• 高通量体外毒理：低内毒素背景满足高灵敏度细胞应激实验

使用方法概要

1. 离心：开盖前瞬时离心，收集管壁冻干粉
2. 复溶：建议用配套Buffer或无菌0.1 % BSA/PBS (pH 7.4) 稀释至 ≥100 µg/mL
3. 稀释保存：二次稀释需含载体蛋白（0.1 % BSA, 10 % FBS 或 5 % HSA），-20 °C/-80 °C分装可稳定3–6个月；避免反复冻融

规格与货期

储存与稳定性

• 冻干粉：-20 °C避光，12个月
• 复溶原液：2–8 °C，≤1周
• 分装溶液：含稳定剂，-20 °C 3个月；-80 °C 6个月

安全与合规声明

本试剂仅供科研用途，非临床诊断或治疗产品。操作需遵守实验室PPE及废弃物管理规范。使用本产品不构成任何专利许可的明示或默示授权。

参考序列：UniProt Q9UK05

Recombinant Human BMP-9-His Protein (Animal-Free Process, E. coli)

Cat. No.: PRP1200 | Purity: >98 % by SDS-PAGE | Tag: His

Suitable for osteogenic induction, chondrogenic commitment and general SMAD-signaling studies in vitro.

Product Snapshot

• Sequence Coverage: Ser320-Arg429, human native sequence (Q9UK05)
• Theoretical Molecular Weight: 12.89 kDa (110 aa); apparent band on SDS-PAGE consistent with expected size
• Activity Assay: ATDC5 cell alkaline phosphatase induction assay, ED₅₀ <0.4 ng/mL
• Endotoxin: ≤0.01 EU/µg by LAL assay, suitable for highly sensitive cell-based experiments
• Form: Lyophilized powder (20 mM sodium citrate, 0.2 M NaCl, pH 3.5), can be reconstituted without additional dialysis
• Host System: E. coli expression system, animal-component free, reducing risk of adventitious viruses

Typical Applications

• Stem Cell Osteogenic Induction: Used in combination with BMP-2/-7, accelerates lineage-specific differentiation of hMSCs towards osteoblasts
• Chondrocyte Phenotype Maintenance: For monitoring ALP activity in ATDC5 or mouse primary chondrocytes
• SMAD Signaling Dynamics: Construction of time-dose models for Smad1/5/8 phosphorylation activation
• High-Throughput In Vitro Toxicology: Low endotoxin background suitable for high-sensitivity cellular stress assays

Usage Overview

1. Centrifugation: Briefly centrifuge the vial before opening to collect the lyophilized powder from the walls
2. Reconstitution: Reconstitute to ≥100 µg/mL using the provided buffer or sterile 0.1% BSA/PBS (pH 7.4)
3. Dilution & Storage: Further dilutions should contain a carrier protein (e.g., 0.1% BSA, 10% FBS, or 5% HSA). Aliquots stored at -20°C/-80°C are stable for 3–6 months; avoid repeated freeze-thaw cycles.

Specifications & Availability

Storage & Stability

• Lyophilized Powder: -20°C protected from light, stable for 12 months
• Reconstituted Stock Solution: 2–8°C, stable for ≤1 week
• Working Aliquots: With stabilizer, stable for 3 months at -20°C; 6 months at -80°C

Safety & Compliance Statement

This reagent is intended for research use only, not for diagnostic or therapeutic applications. Handling should follow laboratory PPE and waste management protocols. Use of this product does not grant any express or implied license under any patents.

Reference Sequence:UniProt Q9UK05