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产品概述

Mouse/Rat TGF beta 1, His tag (Animal-Free)（货号：PRP1171）是一种高纯度、动物源游离的重组蛋白，属于转化生长因子β（TGF-β）超家族。该产品通过大肠杆菌表达系统制备，C端带有6×His标签，便于纯化和检测。其通过严格的质控检测，确保在细胞培养及相关研究中提供高批次间一致性和卓越的性能表现。

产品特性

• 英文名称: Mouse/Rat TGF beta 1, His tag (Animal-Free)
• 货号: PRP1171
• 蛋白长度: 13.8 kDa（SDS-PAGE还原条件下迁移率约为16 kDa）
• 序列信息: 氨基酸序列来源于小鼠（P04202）和大鼠（P17246）TGF β1（Ala279-Cys390）
• 表达宿主: 大肠杆菌
• 纯度: >98%（SDS-PAGE检测）
• 内毒素水平: < 0.1 EU/µg（LAL法测定）
• 活性（ED₅₀）: <0.1 ng/mL，通过抑制HT-2细胞中IL-4依赖性增殖的能力测定

应用领域

• 细胞分化与发育研究
• 免疫调节及炎症反应机制分析
• 组织修复与纤维化模型构建
• 干细胞培养与诱导分化系统

产品形式与复溶建议

本品为冻干粉形式，由含20 mM柠檬酸钠和0.2 M NaCl（pH 3.5）的0.2 µm过滤溶液冻干制成。推荐使用配套复溶缓冲液（Reconstitution Buffer）进行复溶，复溶后浓度建议不低于100 µg/mL，后续可根据实验体系进一步稀释。

保存与运输条件

• 保存条件: 冻干粉在-20°C下可稳定保存1年；复溶后在2-8°C下可保存1周。稀释于含载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液中可在-20°C或-80°C下保存3-6个月，建议避免反复冻融。
• 运输条件: 冰袋运输（蓝冰）

规格与价格

背景信息

转化生长因子β1（TGF-β1）是TGF-β超家族中的重要成员，在细胞生长、分化、免疫调节及组织修复中发挥关键作用。本品为小鼠/大鼠来源的重组蛋白，具有种属高保守性，可广泛用于多种哺乳动物细胞模型的相关机制研究。

注意事项

使用前请离心。本产品仅供科研使用，不适用于人体或临床诊断。使用前请查阅相关文献，了解适用实验体系及潜在知识产权限制。

注：以上信息基于现有数据提供，具体应用条件需用户根据实验体系进一步优化。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Mouse/Rat TGF beta 1, His tag (Animal-Free) (Catalog No.: PRP1171) is a high‑purity, animal‑origin‑free recombinant protein belonging to the transforming growth factor‑beta (TGF‑β) superfamily. The product is produced in an Escherichia coli expression system and carries a C‑terminal 6×His tag for facile purification and detection. It undergoes rigorous quality‑control testing to ensure batch‑to‑batch consistency and superior performance in cell culture and related research.

Product Characteristics

• English Name: Mouse/Rat TGF beta 1, His tag (Animal-Free)
• Catalog No.: PRP1171
• Protein Size: 13.8 kDa (apparent molecular weight ~16 kDa under reducing SDS‑PAGE conditions)
• Sequence Information: Amino‑acid sequence derived from mouse (P04202) and rat (P17246) TGF‑β1 (Ala279‑Cys390)
• Expression Host: Escherichia coli
• Purity: >98 % (SDS‑PAGE analysis)
• Endotoxin Level: < 0.1 EU/µg (LAL assay)
• Activity (ED₅₀): <0.1 ng/mL, determined by inhibition of IL‑4‑dependent proliferation in HT‑2 cells

Applications

• Cell differentiation and developmental studies
• Immunoregulation and inflammation mechanism analysis
• Tissue repair and fibrosis model construction
• Stem cell culture and directed differentiation systems

Product Form and Reconstitution Guidelines

This product is supplied as a lyophilized powder, prepared by freeze‑drying a 0.2 µm‑filtered solution containing 20 mM sodium citrate and 0.2 M NaCl (pH 3.5). Reconstitution is recommended with the provided Reconstitution Buffer; the resulting stock concentration should be ≥100 µg/mL and can be further diluted according to experimental needs.

Storage and Shipping Conditions

• Storage Conditions: Lyophilized powder is stable at –20 °C for up to 1 year; reconstituted material can be stored at 2–8 °C for 1 week. When diluted in buffer containing carrier proteins (e.g., 0.1 % BSA, 10 % FBS, 5 % HSA), aliquots can be stored at –20 °C or –80 °C for 3–6 months. Avoid repeated freeze‑thaw cycles.
• Shipping Conditions: Ice‑pack shipment (blue ice)

Specifications and Pricing

Background Information

Transforming growth factor‑beta 1 (TGF‑β1) is a key member of the TGF‑β superfamily and plays crucial roles in cell proliferation, differentiation, immune regulation, and tissue repair. This recombinant protein derived from mouse/rat exhibits high interspecies conservation and is suitable for mechanistic studies across a wide range of mammalian cell models.

Precautions

Spin down before use. This product is for research purposes only and is not intended for human or clinical diagnostic applications. Consult the relevant literature to determine suitable experimental systems and be aware of any intellectual‑property restrictions.

Note: The above information is based on current data; users should further optimize conditions according to their specific experimental systems.

Note: The information above may change due to product improvements or upgrades; please contact Yakein Corporation for the most up‑to‑date details.