VEGFA 重组人血管内皮生长因子165 人源重组蛋白 细胞

Human VEGF165 Protein (重组人血管内皮生长因子165)

货号: PRP100240 | 产品类型: 无标签人源重组细胞因子

产品概述

本品为高纯度重组人血管内皮生长因子165 (VEGFA) 蛋白，采用人源细胞表达系统制备，严格模拟天然人源VEGF165异构体的生物活性与结构特征。氨基酸序列来源于人源VEGF165 (P15692-4, Met1-Arg191)，与人及猕猴序列高度一致，确保其在跨物种研究中的相关性及可靠性。

关键特性

• 来源种属: 人源 (Human)
• 表达宿主: 人源细胞表达系统，保障蛋白正确折叠及翻译后修饰
• 蛋白长度: 165个氨基酸，理论分子量19.2 kDa；SDS-PAGE显示20-22 kDa条带（受糖基化影响）
• 纯度: >95%（SDS-PAGE分析）
• 活性: 经HUVEC细胞增殖试验验证，ED₅₀为4-16 ng/mL
• 产品形式: 冻干粉，缓冲体系为100 mM Glycine, 10 mM NaCl, pH 7.0

应用方向

本品适用于血管生成研究、内皮细胞功能分析、药物筛选模型构建及组织工程等相关领域，尤其适合需要高一致性人源蛋白的B2B研发与生产平台。

使用说明

• 复溶建议: 使用配套复溶缓冲液，浓度不低于100 µg/mL，后续可根据实验需求稀释
• 保存条件: 冻干粉置于-20℃以下干燥环境；复溶后4℃可保存2-7天，长期保存请分装存于-20℃并建议添加0.1% HSA或BSA
• 运输: 常温运输

背景信息

血管内皮生长因子(VEGF)是内皮细胞特异性肝素结合生长因子，在体内具有诱导血管新生的核心功能。其165氨基酸异构体(VEGF165)为常见功能形式，参与多种生理及病理过程调控。

基因ID: 7422 | UniProt ID: P15692

订购信息

规格	价格（元）	货期
5 μg	1498	现货（次日发货）

注意事项: 本产品仅供科研使用，不适用于临床诊断。使用前请仔细阅读说明书，并遵守相关专利与法规要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human VEGF165 Protein (Recombinant Human Vascular Endothelial Growth Factor 165)

Item No.: PRP100240 | Product Type: Untagged Human Recombinant Cytokine

Product Overview

This product is a high‑purity recombinant human Vascular Endothelial Growth Factor 165 (VEGFA) protein, produced using a human cell expression system, faithfully mimicking the bioactivity and structural characteristics of the native human VEGF165 isoform. The amino‑acid sequence is derived from human VEGF165 (P15692-4, Met1‑Arg191) and is highly homologous to human and rhesus macaque sequences, ensuring relevance and reliability in cross‑species studies.

Key Features

• Source Species: Human
• Expression Host: Human cell expression system, ensuring proper protein folding and post‑translational modifications
• Protein Length: 165 amino acids, theoretical molecular weight 19.2 kDa; SDS‑PAGE shows a 20‑22 kDa band (glycosylation effect)
• Purity: >95% (SDS‑PAGE analysis)
• Activity: Validated by HUVEC proliferation assay, ED₅₀ = 4‑16 ng/mL
• Product Form: Lyophilized powder, buffer composed of 100 mM Glycine, 10 mM NaCl, pH 7.0

Applications

This product is suitable for angiogenesis research, endothelial cell functional analysis, drug‑screening model construction, and tissue engineering, especially for B2B R&D and production platforms requiring highly consistent human protein.

Usage Instructions

• Reconstitution Recommendation: Reconstitute with the provided buffer to a concentration of ≥100 µg/mL; further dilute as needed for experiments
• Storage Conditions: Store lyophilized powder in a dry environment at ≤‑20 °C; after reconstitution, store at 4 °C for 2‑7 days. For long‑term storage, aliquot and keep at –20 °C, preferably with 0.1 % HSA or BSA added
• Shipping: Ambient temperature

Background Information

Vascular Endothelial Growth Factor (VEGF) is an endothelial‑specific heparin‑binding growth factor that plays a central role in inducing angiogenesis in vivo. The 165‑amino‑acid isoform (VEGF165) is the predominant functional form, involved in the regulation of numerous physiological and pathological processes.

Gene ID: 7422 | UniProt ID: P15692

Ordering Information

Specification	Price (CNY)	Lead Time
5 μg	1498	In stock (next‑day shipment)

Caution: This product is for research use only and is not intended for clinical diagnosis. Please read the documentation carefully before use and comply with applicable patents and regulations.

Note: The above information may change due to product optimization or upgrades; please contact Yakeyin Co. for the most current details.