EDF, BCDFII, TRF, 重组蛋白,细胞因子

Mouse IL-5 Protein, His tag (Animal-Free)

产品描述

本品为高纯度、无动物源重组小鼠白细胞介素-5（IL-5）蛋白，C端带有6×His标签。通过大肠杆菌表达系统制备，经严格质控验证，适用于细胞培养等研究应用。

产品特性

• 货号: PRP1170
• 种属: 小鼠
• 表达宿主: 大肠杆菌
• 标签: C端6×His标签
• 纯度: > 98% (SDS-PAGE检测)
• 分子量: 13.93 kDa (计算分子量)；SDS-PAGE迁移范围11-17 kDa
• 内毒素: < 0.1 EU/μg (LAL法测定)

生物活性

通过TF-1细胞增殖实验验证，其诱导增殖的ED₅₀值<0.2 ng/mL。特异性活性> 5 × 10⁶ IU/mg。

产品形式与保存

• 形式: 冻干粉（经0.2 µm过滤，含1X PBS，pH 7.4）
• 复溶建议: 推荐使用配套复溶缓冲液，浓度不低于100 μg/mL
• 保存条件: 冻干粉-20℃保存1年；复溶后2-8℃可保存1周；稀释后-20℃/-80℃可保存3-6个月
• 运输条件: 蓝冰冰袋运输

使用注意事项

使用前请离心。复溶后可根据实验需求进一步稀释，但建议在含载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液中进行，以避免蛋白降解并减少反复冻融。

背景信息

白细胞介素-5（IL-5）是一种主要由Th2细胞产生的细胞因子，在嗜酸性粒细胞分化、活化及存活中起关键作用。属于细胞因子螺旋束家族，与IL-3、IL-4和GM-CSF结构相似。其在免疫调节和炎症反应中具有重要功能。

规格与价格

规格	价格（元）	货期
5 μg	588	现货（次日发货）
20 μg	1498	请咨询
100 μg	3516	请咨询
1 mg	请咨询	请咨询

警告: 本品仅供研究使用，不适用于人类或临床诊断。使用前请查阅相关文献及规定，确保符合实验伦理及法律法规要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-5 Protein, His tag (Animal-Free)

Product Description

This product is a highly purified, animal-free recombinant mouse Interleukin-5 (IL-5) protein with a C-terminal 6×His tag. It is produced using an E. coli expression system, undergoes rigorous quality control validation, and is suitable for research applications such as cell culture.

Product Features

• Catalog Number: PRP1170
• Species: Mouse
• Expression Host: E. coli
• Tag: C-terminal 6×His tag
• Purity: > 98% (as determined by SDS-PAGE)
• Molecular Weight: 13.93 kDa (calculated); migrates between 11-17 kDa on SDS-PAGE
• Endotoxin Level: < 0.1 EU/μg (determined by LAL method)

Biological Activity

Validated by TF-1 cell proliferation assay, with an ED₅₀ value for inducing proliferation of <0.2 ng/mL. Specific activity is > 5 × 10⁶ IU/mg.

Product Form and Storage

• Form: Lyophilized powder (filtered through 0.2 µm, containing 1X PBS, pH 7.4)
• Reconstitution Recommendation: Recommended to reconstitute with the provided buffer to a concentration not less than 100 μg/mL
• Storage Conditions: Lyophilized powder stable for 1 year at -20°C; after reconstitution, stable for 1 week at 2-8°C; after dilution, stable for 3-6 months at -20°C/-80°C
• Shipping Conditions: Shipped with blue ice packs

Usage Notes

Centrifuge the vial prior to opening. After reconstitution, the protein can be further diluted according to experimental needs, but it is recommended to do so in a buffer containing carrier proteins (e.g., 0.1% BSA, 10% FBS, 5% HSA) to avoid protein degradation and minimize freeze-thaw cycles.

Background Information

Interleukin-5 (IL-5) is a cytokine primarily produced by Th2 cells and plays a key role in the differentiation, activation, and survival of eosinophils. It belongs to the helical bundle cytokine family, sharing structural similarities with IL-3, IL-4, and GM-CSF. It has important functions in immune regulation and inflammatory responses.

Specifications and Pricing

Specification	Price (CNY)	Availability
5 μg	588	In Stock (ships next day)
20 μg	1498	Please Inquire
100 μg	3516	Please Inquire
1 mg	Please Inquire	Please Inquire

Warning: This product is for research use only and is not intended for human or diagnostic use. Please review relevant literature and regulations before use to ensure compliance with experimental ethics and legal requirements.

Note: The above information is subject to change due to factors such as product optimization and updates. For specific details, please contact Yakeyin Company.