Hematopoietin-1, Lymphocyte-Ac

产品概览

产品名称：重组人白介素 1‑α（IL‑1α），His‑标签（无动物源）
英文名称：Human IL‑1α Protein, His tag (Animal‑Free)
货号：PRP1003
别名：Hematopoietin‑1, Lymphocyte‑Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte

核心特性

• 表达宿主：大肠杆菌 通过无动物来源发酵工艺获得。
• 蛋白长度：159 个氨基酸（氨基酸序列来源于人源IL‑1α Ser113‑Ala271，UniProt P01583）。
• 预测分子量：约19 kDa。
• 纯度：>98 %（SDS‑PAGE检测）。
• His‑标签：方便亲和层析纯化及下游检测。
• 内毒素水平：<0.1 EU/µg（LAL 法测定），符合细胞培养实验要求。
• 活性：ED₅₀ < 10 pg/mL（促使 D10.G4.1 细胞增殖），比活度约 > 1 × 10⁸ IU/mg。

技术规格

产品形态与缓冲体系

• 形态：冻干粉。
• 复溶缓冲液：无菌 PBS，pH 8.0（随包装提供）。
• 推荐复溶浓度：≥100 µg/mL，使用本产品配套的 Reconstitution Buffer。
• 使用前请离心除去可能的颗粒。

适用应用

本产品定位为核心细胞因子，适用于以下细胞类研究：

• 细胞培养：作为细胞因子补充剂，支持免疫细胞（如巨噬细胞、嗜中性粒细胞、CD8⁺ T 细胞）活化。
• 炎症信号通路研究：诱导 IL‑6、IL‑8、TNF‑α 等下游细胞因子产生，常用于 NF‑κB、MAPK 等通路的激活实验。
• 细胞增殖与分化评估：可测定 D10.G4.1 等白血病细胞系对 IL‑1α 的增殖响应（ED₅₀ < 10 pg/mL）。
• 生物标志物筛选：在体外感染模型或药物筛选平台中评估 IL‑1α 触发的细胞因子网络。
• 体外免疫监测：配合流式细胞术、ELISA、qPCR 等技术，研究 IL‑1α 在免疫监视中的作用。

储存与运输

• 冻干粉：‑20 °C 保存，保质期 1 年。
• 复溶后：2 °C‑8 °C 保存 ≤1 周；若在含 0.1 % BSA、10 % FBS 或 5 % HSA 的稳定剂缓冲液中进一步稀释，可分装后‑20 °C 或‑80 °C 保存 3‑6 个月。
• 避免反复冻融循环，以维持活性。
• 运输方式：使用蓝冰（冰袋）冷链运输。

安全与合规

警告：本产品仅供研究使用，不适用于人类治疗或临床诊断。使用本产品进行的任何实验均需遵守相关专利、许可证及当地法规。因使用本产品可能产生的专利侵权或其他违规行为，供应方不承担责任。

参考信息

基因ID：3552
蛋白质ID：P01583（UniProt）

订购方式

如需大批量采购或特殊包装，请直接联系销售代表获取报价与交期信息。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Recombinant Human Interleukin 1‑α (IL‑1α), His‑tag (Animal‑Free)
English Name: Human IL‑1α Protein, His tag (Animal‑Free)
Catalog No.: PRP1003
Synonyms: Hematopoietin‑1, Lymphocyte‑Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte

Key Features

• Host expression system: Escherichia coli produced via animal‑free fermentation process.
• Protein length: 159 amino acids (sequence derived from human IL‑1α Ser113‑Ala271, UniProt P01583).
• Predicted molecular weight: approximately 19 kDa.
• Purity: >98 % (SDS‑PAGE analysis).
• His‑tag: facilitates affinity chromatography purification and downstream assays.
• Endotoxin level: <0.1 EU/µg (LAL assay), compliant with cell‑culture requirements.
• Activity: ED₅₀ < 10 pg/mL (stimulates D10.G4.1 cell proliferation), specific activity ≈ > 1 × 10⁸ IU/mg.

Technical Specifications

Product Formulation and Buffer System

• Form: Lyophilized powder.
• Reconstitution buffer: sterile PBS, pH 8.0 (provided with the product).
• Recommended reconstitution concentration: ≥100 µg/mL, using the supplied Reconstitution Buffer.
• Centrifuge prior to use to remove any particulates.

Applicable Applications

This product is positioned as a core cytokine, suitable for the following cell‑based studies:

• Cell culture: as a cytokine supplement, supports activation of immune cells (e.g., macrophages, neutrophils, CD8⁺ T cells).
• Inflammatory signaling research: induces downstream cytokines such as IL‑6, IL‑8, TNF‑α, commonly employed in NF‑κB, MAPK pathway activation assays.
• Cell proliferation and differentiation assessment: enables measurement of IL‑1α‑driven proliferative response in leukemia cell lines such as D10.G4.1 (ED₅₀ < 10 pg/mL).
• Biomarker screening: evaluates IL‑1α‑triggered cytokine networks in in‑vitro infection models or drug‑screening platforms.
• In‑vitro immune monitoring: combined with flow cytometry, ELISA, qPCR, etc., to study the role of IL‑1α in immune surveillance.

Storage and Shipping

• Lyophilized powder: store at ‑20 °C, shelf‑life 1 year.
• After reconstitution: store at 2 °C‑8 °C for ≤1 week; if further diluted in stabilizing buffer containing 0.1 % BSA, 10 % FBS, or 5 % HSA, aliquots may be stored at ‑20 °C or ‑80 °C for 3‑6 months.
• Avoid repeated freeze‑thaw cycles to maintain activity.
• Shipping method: cold‑chain transport with gel ice packs.

Safety and Compliance

Warning: This product is for research use only and is not intended for human therapy or clinical diagnosis. All experiments conducted with this product must comply with applicable patents, licenses, and local regulations. The supplier assumes no liability for any patent infringement or other violations arising from use of this product.

Reference Information

Gene ID: 3552
Protein ID: P01583 (UniProt)

Ordering Information

For bulk orders or custom packaging, please contact your sales representative for quotation and lead‑time details.

Note: The above information may change due to product optimization or upgrades; please consult Yakein Company for the most current details.