CXCL8, NAP-1, GCP-1 Protein, H

重组人白介素-8 (IL-8) 蛋白

货号: PRP1022
产品类型: 细胞因子
规格与价格: 10 μg (¥588) | 50 μg (¥1989) | 100 μg (¥3589) | 1 mg (请咨询)
库存状态: 多规格现货，支持次日发货

产品概述

重组人白介素-8 (IL-8) 是一种由大肠杆菌表达系统制备的高纯度蛋白片段，无标签修饰，其氨基酸序列来源于人源 IL-8 (UniProt ID: P10145, Ser28-Ser99)。该产品分子量约为 8 kDa，在还原性 SDS-PAGE 中表现单一条带，经 LAL 法检测内毒素水平低于 1.0 EU/μg，适用于细胞培养及相关体外功能研究。

产品特性

• 来源: 人源
• 表达宿主: 大肠杆菌
• 蛋白长度: 72 个氨基酸（含 N 端甲硫氨酸）
• 分子量: 约 8 kDa
• 纯度: ≥ 98% (SDS-PAGE 检测)
• 标签: 无标签
• 活性验证: 经 ELISA 结合实验验证，可特异性结合小鼠抗人 IL-8 抗体，EC₅₀ 值为 66.4 ng/mL
• 内毒素: < 1.0 EU/μg

形式与复溶

本品为冻干粉形式，缓冲体系为 150 mM NaCl、20 mM Tris，pH 7.0。推荐使用配套复溶缓冲液进行溶解，并建议复溶浓度不低于 100 µg/mL，后续可根据实验体系进一步稀释使用。

保存与运输

保存条件: 冻干粉应在 -20℃ 以下干燥保存；复溶后可在 4℃ 下短期保存（2–7 天），长期保存需置于 -20℃ 以下，建议添加 0.1% HSA 或 BSA 作为载体蛋白并分装以避免反复冻融。
运输条件: 常温运输。

背景信息

白细胞介素-8 (IL-8)，也称 CXCL8、NAP-1 或 GCP-1，属于 CXC 趋化因子家族，是一种主要由单核细胞、巨噬细胞、内皮细胞等多种细胞分泌的炎症因子。其在介导中性粒细胞、T 细胞和嗜碱性粒细胞向炎症部位迁移的过程中发挥关键作用，同时也与某些肿瘤微环境的调控相关。

注意事项

使用前请离心。本品仅限于科研用途，不用于临床诊断、治疗或其他人体用途。用户应自行确保使用方式不侵犯专利权或合规要求。

基因ID: 3576
蛋白ID: P10145 (UniProt)

更多常见别名: CXCL8, MDNCF, NAF, NAP-1, GCP-1, IL-8, LUCT, LYNAP 等。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-8 (IL-8) Protein

Catalog Number: PRP1022
Product Type: Cytokine
Specification & Price: 10 μg (¥588) | 50 μg (¥1989) | 100 μg (¥3589) | 1 mg (Please inquire)
Stock Status: Multiple specifications in stock, next-day shipping supported.

Product Overview

Recombinant Human Interleukin-8 (IL-8) is a high-purity protein fragment produced in an E. coli expression system, without tag modification. Its amino acid sequence is derived from human IL-8 (UniProt ID: P10145, Ser28-Ser99). This product has a molecular weight of approximately 8 kDa, displays a single band in reducing SDS-PAGE, and has an endotoxin level of less than 1.0 EU/μg as determined by the LAL method. It is suitable for cell culture and related in vitro functional studies.

Product Characteristics

• Source: Human
• Expression Host: E. coli
• Protein Length: 72 amino acids (includes N-terminal methionine)
• Molecular Weight: ~8 kDa
• Purity: ≥ 98% (by SDS-PAGE)
• Tag: Tag-free
• Activity Validation: Validated by ELISA binding assay, specifically binds to mouse anti-human IL-8 antibody with an EC₅₀ of 66.4 ng/mL.
• Endotoxin: < 1.0 EU/μg

Formulation & Reconstitution

This product is supplied as a lyophilized powder in a buffer containing 150 mM NaCl, 20 mM Tris, pH 7.0. Reconstitution with the provided buffer is recommended, with a suggested concentration not less than 100 µg/mL. It can be further diluted according to the experimental system.

Storage & Shipping

Storage: Lyophilized powder should be stored dry at -20°C or below. After reconstitution, it can be stored at 4°C for short-term use (2–7 days). For long-term storage, store at -20°C or below. Adding 0.1% HSA or BSA as a carrier protein and aliquoting is recommended to avoid repeated freeze-thaw cycles.
Shipping: Ambient temperature.

Background

Interleukin-8 (IL-8), also known as CXCL8, NAP-1, or GCP-1, belongs to the CXC chemokine family. It is an inflammatory factor primarily secreted by various cells including monocytes, macrophages, and endothelial cells. It plays a key role in mediating the migration of neutrophils, T cells, and basophils to inflammatory sites and is also associated with the regulation of certain tumor microenvironments.

Precautions

Centrifuge before use. This product is for research use only, not intended for clinical diagnosis, treatment, or other human uses. Users are responsible for ensuring that their use does not infringe patents or compliance requirements.

Gene ID: 3576
Protein ID: P10145 (UniProt)

Additional Common Aliases: CXCL8, MDNCF, NAF, NAP-1, GCP-1, IL-8, LUCT, LYNAP, etc.

Note: The above information may be subject to change due to factors such as product optimization and upgrades. Please consult Ya Keyin Company for specific details.