BDNF细胞因子,重组人/小鼠/大鼠脑源性神经营养因子,重组

重组人/小鼠/大鼠脑源性神经营养因子 (BDNF) 蛋白，His标签 (无动物源)

货号：PRP1066

产品概述

本品为高纯度、高活性的重组人/小鼠/大鼠脑源性神经营养因子 (Brain-Derived Neurotrophic Factor, BDNF) 蛋白，通过无动物源的大肠杆菌表达系统制备，并带有C端6×His标签，便于纯化和检测。该产品严格遵循质量控制标准，适用于各类细胞培养等基础研究应用。

产品特性

• 种属来源：人/小鼠/大鼠 (氨基酸序列对应人BDNF蛋白His129-Arg247片段)
• 表达宿主：大肠杆菌 (E.coli)，无动物源
• 分子量：计算分子量为14.45 kDa，SDS-PAGE还原条件下约为14 kDa
• 纯度：经SDS-PAGE检测，纯度 > 98%
• 标签：C端6×His标签
• 内毒素水平：LAL法检测，每1μg蛋白质中EU含量 < 0.1

生物活性

通过其诱导转染有TrkB受体的BaF3细胞增殖的能力来测定生物活性。其半数有效剂量 (ED₅₀) < 2 ng/mL，证明了其卓越的受体结合与信号激活能力。

产品形式与复溶

本品为冻干粉形式，由经过0.2 µm过滤的20 mM柠檬酸钠和0.2 M NaCl (pH 3.5) 溶液冻干而成。

复溶建议：打开前请先以3000 rpm离心5分钟。建议使用无菌ddH₂O进行复溶，浓度不低于100 μg/mL。复溶时请在室温下静置孵育至少20分钟以确保充分溶解。请注意：切勿振荡，剧烈振荡可能导致蛋白变性或活性降低。

保存与运输

保存条件：冻干粉可在-20°C下稳定保存1年。复溶后，蛋白在2°C至8°C下可保存最多1周。若在含有载体蛋白（如0.1% BSA, 10% FBS, 5% HSA）的缓冲液中进行进一步稀释，等分后的蛋白可在-20°C或-80°C下保存3-6个月。请避免反复冻融。

运输条件：全程冰袋（蓝冰）运输，以确保产品的稳定性。

背景信息

脑源性神经营养因子 (BDNF) 是神经营养因子β家族 (NGF-β) 的关键成员。它通过高亲和力结合其特异性受体TrkB，激活下游信号通路（如IRS1/2, PI3K, Akt），在神经元的存活、分化、成熟以及突触可塑性中扮演核心角色，对学习和记忆过程至关重要。本品重组蛋白涵盖了其与受体结合的关键功能域。

UniProt蛋白数据库链接：
人 (P23560):
小鼠 (P21237):
大鼠 (P23363):

别名：ANON2, BULN2

规格与价格

重要提示：本产品仅限于科研使用，不适用于临床诊断、治疗或其他人类用途。使用者应自行确保其应用不侵犯任何专利或许可证权利。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human/Mouse/Rat Brain-Derived Neurotrophic Factor (BDNF) Protein, His Tag (Animal-Free)

Catalog Number: PRP1066

Product Overview

This product is a highly pure and highly active recombinant Human/Mouse/Rat Brain-Derived Neurotrophic Factor (BDNF) protein, produced using an animal-free E. coli expression system and featuring a C-terminal 6×His tag for purification and detection. It is manufactured under strict quality control standards and is suitable for various basic research applications, including cell culture.

Product Features

• Source: Human/Mouse/Rat (Amino acid sequence corresponds to the His129-Arg247 fragment of human BDNF)
• Expression Host: E. coli (Animal-Free)
• Molecular Weight: Calculated molecular mass is 14.45 kDa; migrates at approximately 14 kDa under reducing conditions in SDS-PAGE.
• Purity: > 98% as determined by SDS-PAGE.
• Tag: C-terminal 6×His tag.
• Endotoxin Level: < 0.1 EU per 1 μg of protein, tested by LAL assay.

Biological Activity

The biological activity is measured by its ability to induce proliferation of BaF3 cells transfected with the TrkB receptor. The ED₅₀ is < 2 ng/mL, demonstrating its potent receptor binding and signaling activation capability.

Product Form and Reconstitution

This product is supplied as a lyophilized powder, lyophilized from a 0.2 µm filtered solution of 20 mM Sodium Citrate and 0.2 M NaCl (pH 3.5).

Reconstitution Recommendation: Centrifuge the vial at 3000 rpm for 5 minutes before opening. It is recommended to reconstitute the protein in sterile ddH₂O to a concentration not less than 100 μg/mL. After adding the solvent, allow the vial to stand at room temperature for at least 20 minutes to ensure complete dissolution. Note: Do not vortex, as vigorous agitation may lead to protein denaturation or loss of activity.

Storage and Shipping

Storage: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, the protein can be stored at 2°C to 8°C for up to 1 week. For longer-term storage, when further diluted in a buffer containing a carrier protein (e.g., 0.1% BSA, 10% FBS, 5% HSA), aliquotted protein can be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Shipping: Shipped on blue ice to ensure product stability.

Background Information

Brain-Derived Neurotrophic Factor (BDNF) is a key member of the nerve growth factor-beta (NGF-β) family. It binds with high affinity to its specific receptor, TrkB, activating downstream signaling pathways (e.g., IRS1/2, PI3K, Akt), and plays a central role in neuronal survival, differentiation, maturation, and synaptic plasticity, being crucial for learning and memory processes. This recombinant protein encompasses the key functional domain responsible for receptor binding.

UniProt Database Links:
Human (P23560):
Mouse (P21237):
Rat (P23363):

Aliases: ANON2, BULN2

Specifications and Pricing

Important Note: This product is intended for research use only and is not intended for diagnostic, therapeutic, or other human uses. The user is responsible for ensuring that its application does not infringe upon any patents or license rights.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult YaKeyin Company for specific details.