IL-23 p19细胞因子,重组人白介素-23 p19,重组

重组人IL-23 p19蛋白，His标签（无动物源）

货号：PRP1909

产品概述

本品为高纯度、高活性重组人白介素-23 p19 (IL-23 p19) 蛋白，在大肠杆菌系统中表达，并带有N端6×His标签。该蛋白采用无动物源工艺生产，有效降低了外源因子污染风险，适用于对原料来源有严格要求的细胞培养与免疫学研究。

关键特性

• 高纯度：经SDS-PAGE检测，纯度超过95%，确保实验结果的可靠性与一致性。
• 卓越活性：通过诱导小鼠脾细胞分泌IL-17的生物学活性测定，其ED₅₀值低于0.5 ng/mL，展现出优异的受体结合与信号激活能力。
• 低内毒素：每微克蛋白内毒素含量< 0.1 EU（LAL法检测），最大限度减少对细胞实验的干扰。
• 明确的结构：蛋白序列基于人IL-23 p19 (Arg20-Pro189, Uniprot ID: Q9NPF7)，计算分子量为19.49 kDa，在还原性SDS-PAGE中迁移为17 kDa。

产品规格与定价

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	4698	请咨询
1 mg	请咨询	请咨询

使用说明

复溶建议：本品为冻干粉形式。为获得最佳效果，建议使用配套的复溶缓冲液进行复溶，初始浓度不低于100 µg/mL。用户可根据具体实验方案进行进一步稀释。

保存条件：冻干粉可在-20°C下稳定保存1年。复溶后，于2-8°C可保存1周。若在含有载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液中稀释后分装，于-20°C或-80°C下可保存3-6个月。请避免反复冻融。

注意事项：使用前请先离心。本品仅供研究使用，不应用于人类或临床诊断。

背景信息

白细胞介素-23 (IL-23) 是一种关键的异源二聚体细胞因子，由IL-12p40亚基和其特有的IL-23p19亚基组成。它通过结合由IL-12RB1和IL-23R组成的受体复合物，主要参与Th17细胞的分化与功能调控，并在炎症反应、自身免疫性疾病及宿主防御中发挥核心作用。本研究级重组IL-23 p19蛋白为深入探索IL-23生物学功能及相关信号通路提供了高质量的工具。

运输与质量

本品采用冰袋（蓝冰）运输，以确保产品在运输过程中的稳定性。

注：以上推荐应用仅供参考，不构成专利许可。用户有责任确保其使用方式符合相关法规。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human IL-23 p19 Protein with His Tag (Animal‑Free)

Product No.: PRP1909

Product Overview

This product is a high‑purity, high‑activity recombinant human interleukin‑23 p19 (IL‑23 p19) protein expressed in an Escherichia coli system and bearing an N‑terminal 6×His tag. The protein is produced using an animal‑free process, substantially reducing the risk of exogenous factor contamination, and is suitable for cell culture and immunology studies that require stringent raw‑material provenance.

Key Features

• High Purity: Confirmed by SDS‑PAGE with purity >95%, ensuring reliable and consistent experimental results.
• Outstanding Activity: Demonstrated by inducing mouse splenocyte secretion of IL‑17 with an ED₅₀ 0.5 ng/mL, indicating excellent receptor binding and signal activation.
• Low Endotoxin: Endotoxin level < 0.1 EU per µg protein (LAL assay), minimizing interference with cell‑based assays.
• Defined Structure: Sequence based on human IL‑23 p19 (Arg20‑Pro189, UniProt ID: Q9NPF7), calculated molecular weight 19.49 kDa, migrates at ~17 kDa under reducing SDS‑PAGE conditions.

Product Specification and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock (next‑day shipment)
20 µg	1498	In stock (next‑day shipment)
100 µg	4698	Please inquire
1 mg	Please inquire	Please inquire

Usage Instructions

Reconstitution Recommendation: The product is supplied as a lyophilized powder. For optimal results, reconstitute with the provided buffer to a concentration of ≥100 µg/mL. Users may further dilute according to experimental requirements.

Storage Conditions: Lyophilized powder is stable at –20 °C for up to 1 year. After reconstitution, store at 2–8 °C for up to 1 week. Aliquoted in buffer containing carrier protein (e.g., 0.1 % BSA, 10 % FBS, 5 % HSA) can be stored at –20 °C or –80 °C for 3–6 months. Avoid repeated freeze‑thaw cycles.

Precautions: Centrifuge before use. This product is for research purposes only and is not intended for human or clinical diagnostic applications.

Background

Interleukin‑23 (IL‑23) is a pivotal heterodimeric cytokine composed of the IL‑12p40 subunit and a unique IL‑23p19 subunit. By engaging the receptor complex formed by IL‑12RB1 and IL‑23R, it primarily drives Th17 cell differentiation and function, playing a central role in inflammation, autoimmunity, and host defense. This research‑grade recombinant IL‑23 p19 protein provides a high‑quality tool for probing IL‑23 biology and associated signaling pathways.

Shipping and Quality

The product is shipped with gel ice packs (blue ice) to maintain stability during transit.

Note: The suggested applications are for reference only and do not constitute a patent license. Users are responsible for ensuring their use complies with applicable regulations.

Note: The information above may change due to product optimization or upgrades; please contact YaKeen Inc. for the latest details.