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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 英文名:
Mouse VEGF164 Protein, His tag
- 规格:
5 μg/20 μg/100 μg/1 mg
| 规格: | 5 μg | 产品价格: | ¥588.0 |
|---|---|---|---|
| 规格: | 20 μg | 产品价格: | ¥1498.0 |
| 规格: | 100 μg | 产品价格: | ¥4798.0 |
| 规格: | 1 mg | 产品价格: | 询价 |
重组小鼠源VEGF164蛋白,His标签 (PRP1301)
本产品为高纯度、重组小鼠源VEGF164细胞因子,采用大肠杆菌表达系统生产,带有C端His标签,适用于多种细胞培养及血管生物学研究应用。
产品详情
- 产品名称: VEGF164细胞因子,重组小鼠源血管内皮生长因子,重组蛋白,E.coli,His
- 英文名称: Mouse VEGF164 Protein, His tag
- 货号: PRP1301
- 表达宿主: 大肠杆菌
- 蛋白长度: 165个氨基酸,预测分子量为19.4 kDa
- 纯度: > 99%(经SDS-PAGE验证)
- 蛋白序列来源: 小鼠源VEGF164 (Ala27-Arg190) (UniProt ID: Q00731-2)
- 产品形式: 冻干粉,缓冲液为无菌20 mM Tris,150 mM NaCl,pH 8.5
产品特点与质量指标
- 每微克蛋白内毒素含量 < 0.1 EU(LAL法测定)
- 高纯度重组蛋白,适用于细胞功能实验及体外血管生成研究
- 提供多种规格选择,满足不同实验规模需求
使用注意事项
- 使用前请离心,避免粉末散失
- 推荐使用配套复溶缓冲液进行复溶,浓度不低于106 µg/mL
- 复溶后可根据实验需求进一步稀释
- 复溶后可在4℃保存2-7天,长期保存请置于-20℃及以下,并避免反复冻融
- 为维持长期稳定性,建议添加载体蛋白(0.1% HSA或BSA)
保存与运输
- 保存条件: 冻干粉应在-20℃以下干燥环境中保存
- 运输条件: 蓝冰冰袋运输,保障产品稳定性
背景信息
小鼠血管内皮生长因子(VEGF或VEGFA)是一种强效的血管生成和血管发生调节因子,属于PDGF/VEGF生长因子家族。其通过结合内皮细胞上的VEGF受体(如Flk/KDR)发挥生物学功能,在胚胎发育和成年期血管系统中具有重要作用。
规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 5 μg | 588 | 现货(次日发货) |
| 20 μg | 1498 | 现货(次日发货) |
| 100 μg | 4798 | 现货(次日发货) |
| 1 mg | 请咨询 | 请咨询 |
警告: 本产品仅供科研使用,不适用于人类或临床诊断。使用者应确保不违反任何专利或许可规定。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Recombinant Mouse VEGF164 Protein, His Tag (PRP1301)
This product is a highly pure, recombinant mouse VEGF164 cytokine, produced using an E. coli expression system with a C-terminal His tag. It is suitable for various cell culture and vascular biology research applications.
Product Details
- Product Name: VEGF164 Cytokine, Recombinant Mouse Vascular Endothelial Growth Factor, Recombinant Protein, E. coli, His
- English Name: Mouse VEGF164 Protein, His tag
- Catalog Number: PRP1301
- Expression Host: Escherichia coli (E. coli)
- Protein Length: 165 amino acids, predicted molecular weight of 19.4 kDa
- Purity: > 99% (as determined by SDS-PAGE)
- Protein Sequence Source: Mouse VEGF164 (Ala27-Arg190) (UniProt ID: Q00731-2)
- Form: Lyophilized powder, buffer is sterile 20 mM Tris, 150 mM NaCl, pH 8.5
Features & Quality Control
- Endotoxin level < 0.1 EU per µg of protein (determined by LAL assay)
- High purity recombinant protein, suitable for cell functional assays and in vitro angiogenesis studies
- Available in various sizes to meet different experimental scale requirements
Usage Notes
- Centrifuge the vial prior to opening to avoid loss of powder
- Reconstitution using the provided buffer is recommended to a concentration not less than 106 µg/mL
- Further dilution can be performed according to experimental needs after reconstitution
- After reconstitution, it can be stored at 4°C for 2-7 days. For long-term storage, store at -20°C or below and avoid repeated freeze-thaw cycles
- For long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is recommended
Storage & Shipping
- Storage Conditions: Lyophilized powder should be stored dry at -20°C or below
- Shipping Conditions: Shipped with blue ice packs to ensure product stability
Background
Mouse Vascular Endothelial Growth Factor (VEGF or VEGFA) is a potent regulator of angiogenesis and vasculogenesis, belonging to the PDGF/VEGF growth factor family. It exerts its biological functions by binding to VEGF receptors (such as Flk/KDR) on endothelial cells and plays important roles in embryonic development and the adult vascular system.
Specifications & Pricing
| Specification | Price (CNY) | Availability |
|---|---|---|
| 5 µg | 588 | In Stock (Ships Next Day) |
| 20 µg | 1498 | In Stock (Ships Next Day) |
| 100 µg | 4798 | In Stock (Ships Next Day) |
| 1 mg | Please Inquire | Please Inquire |
Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure compliance with all relevant patents or licensing regulations.
Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.
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文献和实验的生长因子(PDGF)、成纤维细胞生长因子(FGF)、肝细胞生长因子(HGF)、胰岛素样生长因子-I(IGF-1)、IGF-II、白血病抑制因子(LIF)、神经生长因子(NGF)、抑瘤素M(OSM)、血小板衍生的内皮细胞生长因子(PDECGF)、转化生长因子-α(TGF-α)、血管内皮细胞生长因子(VEGF)等。 重组细胞因子是利用基因工程技术在一定的宿主细胞中表达并经过一系列分离纯化工艺制备而成的细胞因子产品,目前已经广泛用于细胞治疗等相关研究领域和医药领域,如肿瘤、感染和造血障碍等疾病的治疗
、Glu、Ser和Thr的区域,且在该区域附近有某些特定的氨基酸,则该蛋白就会不稳定。这些PEST结构域的磷酸化导致钙的结合能力提高,从而利于钙依赖性蛋白酶对蛋白质的降解。这提示可以在缺乏PEST蛋白裂解系统的E.coli中表达PEST富含蛋白。 减少E.coli中重组蛋白裂解的策略有以下几种:(1)将蛋白质靶向细胞周质或培养基[145,186];(2)在较低的温度下培养细菌[187];(3)选用蛋白酶缺陷的菌株[188];(4)构建N-末端或C-末端融合蛋白[186];(5)将目的基因多拷贝串联
曲线。 3. 根据样品 OD 值在该曲线图上查出相应 VEGF 含量。 试剂盒性能 1. 灵敏度 : 最小的VEGF 检测浓度小于 14 pg/ml。 2. 特异性: 可同时检测重组或天然的小鼠 VEGF 。 不与 小鼠其它细胞因子有交叉反应。 3. 重复性:板内、板见变异系数均小于9.7%。 注意事项 1. 以上标准孔及待 测 样品均建议做复孔,每次测定应同时 做 标准曲线。
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