- ￥3520
- novoprotein
- CX73
- 2025年12月29日
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- 库存：
大量
- 供应商：
上海研卉生物科技有限公司
- 英文名：
VEGF164 (Yeast)
- 规格：
50ug
Recombinant Mouse VEGF-A164/Vascular endothelial growth factor A (CX73)
产品说明(Description)
Recombinant Mouse Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190 is expressed.
Accession #: Q00731-2
Known as: Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGFA164; VEGF164
制剂（Formulation）
Lyophilized from a 0.2 μm filtered solution of 20mM PB,250mM NaCl.pH7.0.
质量控制（Quality Control）
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶（Reconstitution）
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
背景（Background）
Mouse Vascular endothelial growth factor (VEGF or VEGF-A), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF/VEGF growth factor family that is characterized by a cystine knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 120, 164 and 188 aa found in mouse. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-/KDR) on endothelial cells.Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells.It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.
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文献和实验小鼠 血管内皮生长因子（VEGF) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 血管内皮生长因子（VEGF) 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 血管内皮生长因子（VEGF) 水平。用纯化的 小鼠 血管内皮生长因子（VEGF) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 小鼠 血管内皮生长因子
, Virus, Model Organisms Arabidopsis, C. Elegans, Yeast... Molecular Biology Carbohydrate, DNA, Cloning, PCR, RNA, Protein... Neuroscience Neuroanatomy, Neurophysiology... Physiology Hematology... Plant Biology Photosynthesis, Tissue
克隆 已经制备好的双链cDNA和一般DNA一样,可以插入到质粒或噬菌体中,为此,首先必需连接上接头(Linker),接头可以是限制性内切酶识别位点片段,也可以利用末端转移酶在载体和双链cDNA的末端接上一段寡聚dG和dC或dT和dA尾巴,退火后形成重组质粒,并转化到宿主菌中进行扩增。合成的cDNA也可以经PCR扩增后再克隆入适当载体。 第二节 动植物组织mRNA提取 一、材料 水稻叶片或小鼠肝组织。 二、设备 研钵，冷冻台式高速离心机，低温冰箱，冷冻真空干燥器，紫外
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