IL-8 重组人白介素-8 人源重组蛋白 细胞因子 PRP1

产品详情：重组人白介素-8 (IL-8) 蛋白，His标签（无动物源）

产品概述

本品为高纯度、高活性的重组人白介素-8 (IL-8) 蛋白，采用无动物源成分的工艺在大肠杆菌表达系统中生产，并在C末端融合了6×His标签，便于下游应用。该蛋白是趋化因子研究领域的核心工具，其氨基酸序列对应于人源IL-8 (UniProt ID: P10145) 的Ser28-Ser99片段，是巨噬细胞分泌的72个氨基酸成熟体形式。

关键特性

• 高纯度 & 高比活性：经SDS-PAGE验证，纯度 > 98%。通过其趋化吸引外周血单核细胞(PBMC)的能力测定，其生物学活性ED₅₀值 < 2 ng/mL。
• 极低内毒素：通过LAL法测定，每微克蛋白的内毒素含量 < 0.1 EU，极大降低了由内毒素引起的实验背景风险。
• 无动物源表达系统：在大肠杆菌中重组表达，生产过程中无任何动物源成分，确保了产品的批次间一致性并降低了外源因子污染的风险。
• 明确的分子量与形态：计算分子量为9.32 kDa；在还原性SDS-PAGE中迁移为约11 kDa。

产品信息

货号	产品名称	种属	表达宿主	标签
PRP1096	Human IL-8 Protein, His tag (Animal-Free)	人 (Human)	大肠杆菌 (E. coli)	C-端 6×His

应用方向

本品是研究炎症反应、细胞迁移与免疫调节机制的理想工具，特别适用于：

• 趋化作用与细胞迁移 assay（例如Boyden chamber实验）
• 中性粒细胞或其它免疫细胞的功能研究
• 体外细胞培养与刺激实验
• 蛋白质印迹 (Western Blot) 及ELISA标准品的制备

使用说明

• 复溶：本品为冻干粉形式。开盖前请先离心。推荐使用配套的复溶缓冲液（Reconstitution Buffer）进行复溶，且复溶浓度不低于100 µg/mL，以获得最佳稳定性。复溶后可据实验需求进行进一步稀释。
• 保存：
  • 冻干粉：-20°C干燥保存，有效期1年。
  • 复溶后：于2-8°C可保存最多1周。
  • 稀释后：若在含有载体蛋白（如0.1% BSA, 10% FBS, 5% HSA）的缓冲液中进一步稀释，等分后于-20°C或-80°C可保存3-6个月。请避免反复冻融。

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货，次日发货
20 μg	1498	现货，次日发货
100 μg	3216	请咨询
1 mg	请咨询	请咨询

背景信息

白介素-8 (IL-8)，也被称为CXCL8，是一种重要的CXC趋化因子。它由多种细胞类型（如巨噬细胞、上皮细胞、内皮细胞）产生，其主要功能是作为强效的化学吸引物，招募中性粒细胞、嗜碱性粒细胞和T细胞到炎症部位，在先天免疫应答和炎症过程中扮演着核心角色。

注意事项

警告：本品仅限于科学研究用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。

运输条件：本品采用冰袋（蓝冰）运输，以确保产品的稳定性。

如需了解更多信息，如蛋白序列详情，请访问UniProt数据库：P10145 (IL8_HUMAN)

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Details: Recombinant Human Interleukin-8 (IL-8) Protein, His Tag (Animal-Free)

Product Overview

This product is a high-purity, high-activity recombinant human Interleukin-8 (IL-8) protein, produced in an E. coli expression system using an animal-free process and fused with a C-terminal 6×His tag for downstream applications. This protein is a core tool in chemokine research, and its amino acid sequence corresponds to the Ser28-Ser99 fragment of human IL-8 (UniProt ID: P10145), representing the 72-amino acid mature form secreted by macrophages.

Key Features

• High Purity & High Specific Activity: Purity > 98% as verified by SDS-PAGE. Its biological activity, determined by its ability to chemoattract peripheral blood mononuclear cells (PBMCs), has an ED₅₀ value of < 2 ng/mL.
• Ultra-Low Endotoxin: Endotoxin level is < 0.1 EU per µg of protein as measured by the LAL method, significantly reducing the risk of experimental background caused by endotoxins.
• Animal-Free Expression System: Recombinantly expressed in E. coli with no animal-derived components used in the production process, ensuring batch-to-batch consistency and reducing the risk of contamination by adventitious agents.
• Defined Molecular Weight & Form: Calculated molecular mass is 9.32 kDa; migrates at approximately 11 kDa under reducing SDS-PAGE conditions.

Product Information

Catalog Number	Product Name	Species	Expression Host	Tag
PRP1096	Human IL-8 Protein, His tag (Animal-Free)	Human	E. coli	C-terminal 6×His

Applications

This product is an ideal tool for studying inflammatory responses, cell migration, and immune regulation mechanisms, particularly suitable for:

• Chemotaxis and cell migration assays (e.g., Boyden chamber assay)
• Functional studies of neutrophils or other immune cells
• In vitro cell culture and stimulation experiments
• Preparation of standards for Western Blot and ELISA

Usage Instructions

• Reconstitution: This product is supplied as a lyophilized powder. Centrifuge the vial briefly before opening. It is recommended to reconstitute using the provided Reconstitution Buffer to a concentration not less than 100 µg/mL for optimal stability. The reconstituted solution can be further diluted according to experimental needs.
• Storage:
  • Lyophilized powder: Store desiccated at -20°C. Stable for 1 year.
  • Reconstituted solution: When stored at 2-8°C, it is stable for up to 1 week.
  • Diluted solution: If further diluted in a buffer containing a carrier protein (e.g., 0.1% BSA, 10% FBS, 5% HSA), aliquot and store at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Specifications & Pricing

Specification	Price (RMB)	Availability
5 μg	588	In stock, ships next day
20 μg	1498	In stock, ships next day
100 μg	3216	Please inquire
1 mg	Please inquire	Please inquire

Background

Interleukin-8 (IL-8), also known as CXCL8, is a key CXC chemokine. It is produced by various cell types (such as macrophages, epithelial cells, and endothelial cells). Its primary function is to act as a potent chemoattractant, recruiting neutrophils, basophils, and T cells to sites of inflammation, playing a central role in innate immune responses and inflammatory processes.

Notes

Warning: This product is for research use only, not intended for human or diagnostic use. Users should ensure their application does not infringe any patents or license agreements.

Shipping Conditions: This product is shipped with ice packs (blue ice) to ensure stability.

For more information, such as detailed protein sequence, please visit the UniProt database: P10145 (IL8_HUMAN)

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please contact YaKeyin Company.