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重组小鼠白介素-4（无动物源）

产品名称：Mouse IL-4 protein (PRP1962)
产品类型：细胞因子及蛋白核心产品
产品形态：高纯度、无标签重组蛋白冻干粉

产品概述

重组小鼠白介素-4（IL-4）是一种由大肠杆菌表达系统重组制备的细胞因子，氨基酸序列来源于小鼠IL-4（NP_067258.1，His 23-Ser 140）。该蛋白无动物源成分，适用于细胞培养等生物医学研究场景，尤其适用于B细胞、T细胞功能研究及免疫调节机制探索。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 > 95%，确保实验结果的可靠性。
• 无标签设计：避免标签干扰，更贴近天然蛋白的生物学功能。
• 低内毒素：LAL法检测内毒素含量 < 1 EU/μg，减少对细胞实验的潜在影响。
• 高生物活性：通过NFS-60细胞增殖实验验证，比活性 > 1.86 × 10⁵ IU/mg，ED₅₀为5.37 ng/mL。

技术参数

应用方向

IL-4在免疫调节中具有多重功能，包括：

• 促进B细胞增殖及IgG1、IgE抗体类别转换；
• 诱导Th2细胞分化，调节适应性免疫应答；
• 参与单核细胞/巨噬细胞表面CD23表达调控。

保存与运输

保存条件：冻干粉-20°C保存1年；复溶后2-8°C可保存1周，添加稳定剂后-20°C或-80°C可分装保存3-6个月。
运输方式：冰袋运输（蓝冰），保障产品活性。

产品规格与价格

注意事项

使用前请离心。复溶后避免反复冻融。本产品仅用于科研，不适用于临床诊断或人体使用。

基因ID：16189 | UniProt链接：P07750

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin-4 (Animal‑Free)

Product Name: Mouse IL-4 protein (PRP1962)
Product Type: Cytokine and Core Protein Product
Product Form: High‑purity, tag‑free recombinant protein lyophilized powder

Product Overview

Recombinant mouse interleukin‑4 (IL‑4) is a cytokine produced in an Escherichia coli expression system, with an amino‑acid sequence derived from mouse IL‑4 (NP_067258.1, His 23‑Ser 140). The protein contains no animal‑derived components and is suitable for cell‑culture and other biomedical research applications, especially for studies on B‑cell and T‑cell functions and immune‑regulatory mechanisms.

Key Features

• High Purity: SDS‑PAGE analysis shows purity > 95%, ensuring reliable experimental results.
• Tag‑Free Design: Avoids tag interference, closely reflecting the native protein’s biological function.
• Low Endotoxin: LAL assay detects endotoxin < 1 EU/µg, minimizing potential impact on cell assays.
• High Biological Activity: Validated by NFS‑60 cell proliferation assay, specific activity > 1.86 × 10⁵ IU/mg, ED₅₀ = 5.37 ng/mL.

Technical Specifications

Applications

IL‑4 plays multiple roles in immune regulation, including:

• Promotion of B‑cell proliferation and class switching to IgG1 and IgE antibodies;
• Induction of Th2 cell differentiation, modulating adaptive immune responses;
• Regulation of CD23 expression on monocytes/macrophages.

Storage and Shipping

Storage Conditions: Lyophilized powder stored at –20 °C for up to 1 year; after reconstitution, stable at 2–8 °C for 1 week, or aliquoted with stabilizer at –20 °C or –80 °C for 3–6 months.
Shipping Method: Shipped on ice packs (blue ice) to preserve product activity.

Product Specifications and Pricing

Precautions

Centrifuge before use. Avoid repeated freeze‑thaw cycles after reconstitution. This product is for research use only and is not intended for clinical diagnosis or human administration.

Gene ID: 16189 | UniProt Link: P07750

Note: The above information may change due to product optimization or upgrades; please contact Yakoin Co. for the latest details.