GM-CSF 重组人粒细胞-巨噬细胞集落刺激因子 人源重组蛋

重组人粒细胞-巨噬细胞集落刺激因子 (GM-CSF)，His标签 (无动物源)

货号： PRP2016
产品名称： Human GM-CSF protein, His tag (Animal-Free)
产品形式： 冻干粉

产品概述

本品为采用重组DNA技术，在人胚胎肾细胞中表达的高纯度、无动物源的重组人粒细胞-巨噬细胞集落刺激因子（GM-CSF）蛋白。其氨基酸序列来源于人源GM-CSF (NP_000749.2, Met1-Glu144)，并在C端融合有多聚组氨酸标签(His-tag)，便于纯化与检测。该产品经过严格的质量控制，适用于各类细胞培养及相关生物医学研究。

关键特性

• 高纯度： 经SDS-PAGE检测，纯度大于98%。
• 高生物活性： 采用TF-1细胞增殖试验测定，其ED₅₀为0.3187 ng/mL。
• 低内毒素： 采用LAL法检测，每1μg蛋白质中的内毒素(EU)含量小于1.0。
• 无动物源表达系统： 生产过程避免使用动物源性原料，降低了外源因子污染的风险。

技术参数

• 种属： 人
• 表达宿主： 人胚胎肾细胞
• 蛋白长度： 127个氨基酸
• 预测分子量： 14.5 kDa
• 表观分子量（SDS-PAGE）： 约23.8 kDa (因糖基化修饰)
• 标签： C端-His标签
• 冻干缓冲液： 无菌PBS，pH 7.4

使用说明

复溶建议： 开盖前请先离心。推荐使用产品配套的复溶缓冲液进行复溶，复溶浓度建议不低于100 µg/mL。用户可根据具体实验需求进行进一步稀释。

保存条件： 冻干粉应在-20℃以下干燥环境保存。复溶后，于4℃可保存2-7天；如需长期保存，请置于-20℃以下，并建议添加载体蛋白（如0.1% HSA或BSA），分装保存以避免反复冻融。

运输条件： 冰袋（蓝冰）运输。

背景信息

粒细胞-巨噬细胞集落刺激因子（GM-CSF）是一种重要的造血生长因子，由活化T细胞、巨噬细胞、内皮细胞等多种细胞产生。它能刺激粒细胞、巨噬细胞、嗜酸性粒细胞及红细胞等多系造血前体细胞的增殖、分化与活化。在临床研究与应用中，GM-CSF与骨髓移植后的造血重建、外周血祖细胞的动员、以及某些白血病的治疗等领域密切相关。

基因ID： 1437
UniProt ID： P04141

规格与价格

警告： 本产品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), His Tag (Animal-Free)

Catalog Number: PRP2016
Product Name: Human GM-CSF protein, His tag (Animal-Free)
Formulation: Lyophilized Powder

Product Overview

This product is a high-purity, animal-free recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) protein produced using recombinant DNA technology expressed in human embryonic kidney cells. Its amino acid sequence is derived from human GM-CSF (NP_000749.2, Met1-Glu144) and is fused with a polyhistidine tag (His-tag) at the C-terminus for convenient purification and detection. This product undergoes rigorous quality control and is suitable for various cell culture applications and related biomedical research.

Key Features

• High Purity: >98% as determined by SDS-PAGE analysis.
• High Bioactivity: Measured by its ability to stimulate TF-1 cell proliferation. The ED₅₀ for this effect is typically 0.3187 ng/mL.
• Low Endotoxin: <1.0 EU per 1 μg of protein, as determined by LAL assay.
• Animal-Free Expression System: The production process avoids the use of animal-derived materials, reducing the risk of contamination by adventitious agents.

Technical Parameters

• Species: Human
• Expression Host: Human Embryonic Kidney Cells
• Protein Length: 127 amino acids
• Predicted Molecular Weight: 14.5 kDa
• Apparent Molecular Weight (SDS-PAGE): Approximately 23.8 kDa (due to glycosylation)
• Tag: C-terminal His Tag
• Lyophilization Buffer: Sterile PBS, pH 7.4

Usage Instructions

Reconstitution Recommendation: Centrifuge the vial prior to opening. It is recommended to reconstitute the protein using the provided reconstitution buffer to a concentration of not less than 100 µg/mL. Users can perform further dilutions according to specific experimental requirements.

Storage Conditions: The lyophilized powder should be stored in a dry environment at -20°C or below. After reconstitution, it can be stored at 4°C for 2-7 days. For long-term storage, aliquot and store at -20°C or below, and it is advisable to add a carrier protein (e.g., 0.1% HSA or BSA) to avoid repeated freeze-thaw cycles.

Shipping Conditions: Shipped with ice packs (blue ice).

Background Information

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a crucial hematopoietic growth factor produced by various cells, including activated T cells, macrophages, and endothelial cells. It stimulates the proliferation, differentiation, and activation of myeloid lineage hematopoietic precursor cells, such as granulocytes, macrophages, eosinophils, and erythrocytes. In clinical research and applications, GM-CSF is closely related to areas such as hematopoietic reconstitution post-bone marrow transplantation, mobilization of peripheral blood progenitor cells, and the treatment of certain leukemias.

Gene ID: 1437
UniProt ID: P04141

Specifications & Pricing

Warning: This product is for research use only and is not intended for human or diagnostic use. Users should ensure that their application does not infringe any patents or license agreements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yaken Company for specific details.