BMP-9 重组人骨形态发生蛋白9 人源重组蛋白 细胞因子

Human BMP-9 Protein, His tag (Animal-Free)

货号：PRP1200 | 人源重组骨形态发生蛋白9

本产品为高纯度、高活性、无动物源（Animal-Free）的重组人骨形态发生蛋白9（BMP-9）。该蛋白通过大肠杆菌表达系统重组表达，并带有His标签，便于纯化与检测。其严格的质控体系确保了批次间的高度一致性，是进行细胞分化、骨骼代谢及信号转导等研究的可靠工具。

产品特性

• 高生物活性：经ATDC5细胞碱性磷酸酶（ALP）诱导实验测定，其半数有效剂量（ED₅₀）< 0.4 ng/mL，表现出卓越的诱导成骨细胞分化能力。
• 极高纯度：经SDS-PAGE凝胶电泳检测，纯度 > 98%。
• 低内毒素：通过LAL法测定，每微克蛋白内毒素含量 < 0.01 EU，极大降低了由内毒素引起的非特异性细胞反应风险。
• 无动物源表达：整个生产过程避免使用任何动物源性原料，有效规避病毒污染风险，适用于对安全性要求极高的研究。

产品描述

本产品是由人源BMP-9基因（UniProt: Q9UK05）编码的Ser320-Arg429片段重组而成，由110个氨基酸组成，预测分子量为12.89 kDa。冻干前缓冲液为无菌的20mM sodium citrate, 0.2 M NaCl, pH 3.5。

背景信息

骨形态发生蛋白9（BMP-9），也称为生长分化因子2（GDF2），是转化生长因子-β（TGF-β）超家族中的一种多功能细胞因子。它是体内诱导成骨细胞分化的最有效因子之一，且其活性不受常见BMP抑制剂的干扰。BMP-9通过结合TGF-β受体并激活SMAD信号通路，在骨骼发育、血管生成和能量稳态等多种生理过程中发挥关键作用。

使用说明

• 复溶：推荐使用我们提供的专用复溶缓冲液（Reconstitution Buffer）进行复溶，以获得不低于100 µg/mL的储备液浓度。开盖前请先离心，以确保产品全部沉于管底。
• 保存：冻干粉可在-20°C下稳定保存1年。复溶后的蛋白在2-8°C下可保存1周。若在含有载体蛋白（如0.1% BSA, 10% FBS, 5% HSA）的缓冲液中进一步稀释并分装，于-20°C或-80°C下可保存3-6个月。请避免反复冻融。

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	请咨询
100 μg	5998	请咨询
1 mg	请咨询	请咨询

运输条件

本品采用蓝冰冰袋运输，以确保产品在整个运输过程中保持稳定的低温环境。

警告：本品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

基因ID：2658 | 别名：BMP9, BMP-9, bone morphogenetic protein 9, GDF2, NAP-1

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human BMP-9 Protein, His tag (Animal-Free)

Catalog No.: PRP1200 | Recombinant Human Bone Morphogenetic Protein 9

This product is a high‑purity, high‑activity recombinant human BMP‑9 (Bone Morphogenetic Protein 9) that is Animal‑Free. The protein is expressed in an Escherichia coli system and carries a His‑tag for convenient purification and detection. A stringent quality‑control system ensures high batch‑to‑batch consistency, making it a reliable tool for studies of cell differentiation, bone metabolism, and signal transduction.

Product Features

• High biological activity: Determined by ALP induction assay in ATDC5 cells, the median effective dose (ED₅₀) is < 0.4 ng/mL, demonstrating outstanding ability to induce osteoblastic differentiation.
• Ultra‑high purity: SDS‑PAGE analysis shows purity > 98%.
• Low endotoxin: Measured by LAL assay, endotoxin level is < 0.01 EU per µg of protein, markedly reducing the risk of non‑specific cellular responses caused by endotoxin.
• Animal‑free expression: The entire production process avoids any animal‑derived raw materials, effectively eliminating viral contamination risks and suitable for studies with stringent safety requirements.

Product Description

This product is a recombinant fragment encoded by the human BMP‑9 gene (UniProt: Q9UK05), comprising residues Ser320‑Arg429, a 110‑amino‑acid segment with a predicted molecular weight of 12.89 kDa. The lyophilization buffer is sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.

Background Information

Bone morphogenetic protein 9 (BMP‑9), also known as growth differentiation factor 2 (GDF2), is a multifunctional cytokine belonging to the transforming growth factor‑β (TGF‑β) superfamily. It is one of the most potent endogenous inducers of osteoblastic differentiation and its activity is not hindered by common BMP antagonists. BMP‑9 binds TGF‑β receptors and activates SMAD signaling, playing pivotal roles in skeletal development, angiogenesis, and energy homeostasis.

Usage Instructions

• Reconstitution: It is recommended to dissolve the lyophilizate with our proprietary Reconstitution Buffer to achieve a stock concentration of at least 100 µg/mL. Centrifuge the vial before opening to ensure all product has settled at the bottom.
• Storage: The lyophilized powder is stable for 1 year at –20 °C. Reconstituted protein can be stored for 1 week at 2–8 °C. If further diluted and aliquoted in a buffer containing carrier proteins (e.g., 0.1 % BSA, 10 % FBS, 5 % HSA), it can be stored at –20 °C or –80 °C for 3–6 months. Avoid repeated freeze‑thaw cycles.

Specifications & Pricing

Specification	Price (CNY)	Lead Time
5 μg	588	In stock (next‑day shipment)
20 μg	1498	Please inquire
100 μg	5998	Please inquire
1 mg	Please inquire	Please inquire

Shipping Conditions

The product is shipped using a blue dry‑ice pack to maintain a stable low‑temperature environment throughout transit.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements.

Gene ID: 2658 | Aliases: BMP9, BMP-9, bone morphogenetic protein 9, GDF2, NAP-1

Note: The above information may change due to product optimization or upgrades; please contact Yakein for the most current details.