IL-4 - Human IL-4 Protein, His

产品概述

产品名称：Human IL-4 Protein, His tag (Animal‑Free)
货号：PRP1085
别名／检索关键词：IL‑4、BCGF、BCDF、B‑cell Stimulating Factor (BSF‑1)、重组人白介素‑4（His‑标签、无动物源）

核心特性

• 采用大肠杆菌表达体系，C‑末端携带6×His标签，便于亲和层析纯化。
• 纯度 > 98%（SDS‑PAGE 检测），分子量 15.9 kDa（还原条件下迁移率约 12 kDa）。
• 活性通过诱导 TF‑1 细胞增殖测定，ED₅0 < 0.2 ng/mL，特异性活性约 > 2.8 × 10⁷ IU/mg。
• 内毒素水平 < 0.1 EU/µg（LAL 法），满足细胞培养的低内毒素需求。
• 完整的动物源自由（Animal‑Free）生产流程，适用于对动物成分敏感的实验体系。

应用领域

本产品定位于细胞因子及蛋白类研发，尤其在以下细胞培养实验中常用：

• TF‑1 或其它白血病细胞系的增殖刺激实验。
• Th2 分化与过敏/哮喘模型的信号通路研究。
• B 细胞活化、增殖及 IgE 类转换的调控实验。
• 免疫调节因子相互作用（如 IL‑4 与 IL‑13、STAT6）的体外验证。
• ELISA、Western blot、流式细胞仪等下游检测的标准品或阳性对照。

产品形态与使用说明

本品为冻干粉，包装于 0.2 µm 过滤的 1×PBS（pH 8.0）溶液中。建议使用配套的 Reconstitution Buffer 重悬，最终浓度不低于 100 µg/mL。

使用前处理：打开瓶盖后请先以 4 °C、10 000 g 离心 5 min，取上清进行实验。

推荐缓冲体系（示例）

规格与价格

储存与运输

• 冻干状态：建议在 –20 °C 保存，保质期 1 年。
• 复溶后：2 °C–8 °C 可保存 ≤ 1 周；如需长期保存，请在含 0.1% BSA、10% FBS 或 5% HSA 的缓冲液中等分，随后在 –20 °C 或 –80 °C 再保存 3–6 个月。
• 避免反复冻融循环，以免影响活性。
• 运输方式：采用蓝冰包装的冷链运输，确保全程低温。

警示与合规

本产品仅供科研使用，**不用于人类治疗或临床诊断**。使用本品进行的任何研究必须遵守当地法规与专利要求。若因使用本品导致的专利侵权或其他合规风险，供应商概不负责。

参考信息

基因/蛋白 ID：P05112（Human IL‑4）
氨基酸序列来源：IL‑4(His25‑Ser153)（UniProt: P05112）
分子量：15.9 kDa（计算值）

背景简介

白细胞介素‑4（IL‑4）是活化的 T 细胞、肥大细胞、嗜碱/嗜中性/嗜酸性粒细胞分泌的关键细胞因子。它在 Th2 免疫反应的启动与维持中发挥核心作用，关联过敏性疾病与哮喘。IL‑4 还能促进 B 细胞增殖、存活以及 IgE 类转换，是 B 细胞刺激因子（BSF‑1）的重要成员。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Human IL-4 Protein, His tag (Animal‑Free)
Catalog Number: PRP1085
Synonyms/Keywords: IL‑4, BCGF, BCDF, B‑cell Stimulating Factor (BSF‑1), Recombinant Human Interleukin‑4 (His‑tag, Animal‑Free)

Key Features

• Expressed in an E. coli expression system with a C‑terminal 6×His tag for affinity chromatography purification.
• Purity > 98% (by SDS‑PAGE), molecular weight 15.9 kDa (approximately 12 kDa under reducing conditions).
• Bioactivity measured by its ability to induce TF‑1 cell proliferation; ED₅0 < 0.2 ng/mL, specific activity approximately > 2.8 × 10⁷ IU/mg.
• Endotoxin level < 0.1 EU/µg (by LAL assay), meeting low endotoxin requirements for cell culture.
• Complete Animal‑Free manufacturing process, suitable for experimental systems sensitive to animal-derived components.

Applications

This product is intended for cytokine and protein research, commonly used in the following cell culture experiments:

• Proliferation stimulation assays using TF‑1 or other leukemia cell lines.
• Signaling pathway studies in Th2 differentiation and allergy/asthma models.
• Regulation experiments for B cell activation, proliferation, and IgE class switching.
• In vitro validation of immunomodulatory factor interactions (e.g., IL‑4 with IL‑13, STAT6).
• As a standard or positive control for downstream assays such as ELISA, Western blot, and flow cytometry.

Form and Usage

This product is supplied as a lyophilized powder in a 0.2 µm filtered 1×PBS (pH 8.0) solution. Reconstitution using the provided Reconstitution Buffer is recommended to achieve a final concentration of at least 100 µg/mL.

Pre-use Handling: After opening the vial, centrifuge at 4 °C, 10 000 g for 5 min and use the supernatant for experiments.

Recommended Buffer Systems (Examples)

Specifications and Pricing

Storage and Shipping

• Lyophilized State: Store at –20 °C; shelf life 1 year.
• After Reconstitution: Store at 2 °C–8 °C for ≤ 1 week; for long-term storage, aliquot in buffer containing 0.1% BSA, 10% FBS, or 5% HSA and store at –20 °C or –80 °C for 3–6 months.
• Avoid repeated freeze-thaw cycles to maintain activity.
• Shipping Method: Shipped with blue ice to ensure a consistent cold chain.

Cautions and Compliance

This product is for research use only, **not intended for human therapeutic use or clinical diagnosis**. Any research conducted using this product must comply with local regulations and patent requirements. The supplier is not responsible for patent infringement or other compliance risks arising from the use of this product.

Reference Information

Gene/Protein ID: P05112 (Human IL‑4)
Amino Acid Sequence Source: IL‑4(His25‑Ser153) (UniProt: P05112)
Molecular Weight: 15.9 kDa (calculated)

Background

Interleukin‑4 (IL‑4) is a key cytokine secreted by activated T cells, mast cells, and basophilic/neutrophilic/eosinophilic granulocytes. It plays a central role in initiating and maintaining Th2 immune responses, associated with allergic diseases and asthma. IL‑4 also promotes B cell proliferation, survival, and IgE class switching, and is a key member of B cell stimulating factors (BSF‑1).

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact AcroBiosystems for specific details.