PDGF-BB - Human PDGF-BB protei

产品概述

Human PDGF‑BB (血小板来源生长因子‑BB) 重组蛋白（货号 PRP1958）是针对细胞因子及蛋白核心产品线研发的高纯度研究试剂，适用于人源细胞模型的功能性实验。产品以大肠杆菌表达体系生产，提供冻干粉形式，便于长期保存与快速复溶。

核心特性

• 分子量：12.3 kDa
• 纯度：> 98 %（SDS‑PAGE 鉴定）
• 活性测定：在 Ba/F3‑c3T3 细胞增殖实验中，ED₅₀约为 2.588 ng/mL，重组蛋白的特异活性约为 > 3.86 × 10⁵ IU/mg
• 表达宿主：大肠杆菌（E. coli）
• 产品形态：冻干粉（Lyophilized powder）
• 保存建议：避光并储存于 ‑20 °C，确保 12 个月有效期
• 运输方式：冷链（蓝色冰袋）

适用研究领域

PDGF‑BB 在多种细胞生物学和组织工程实验中发挥关键作用，常用于以下研究：

• 成纤维细胞、平滑肌细胞及骨细胞的增殖、迁移与存活性研究
• 血管生成（angiogenesis）模型及血管重塑相关信号通路探索
• 骨再生与修复的体外评价，特别是与成骨细胞、间充质干细胞的协同作用
• 血流动力学与血管收缩调控（如 CX43 介导的血管反应）相关的出血性休克模型
• 阴茎海绵体平滑肌细胞（CCSMCs）功能研究，关注 PDGFR 介导的信号通路

技术参数

参数	数值/说明
基因ID	5155
蛋白质ID (Uniprot)	P01127
目标物种	人 (Human)
表达系统	E.coli 重组表达
纯度检测	>98 %（SDS‑PAGE）
活性指标	ED50 = 2.588 ng/mL（Ba/F3‑c3T3 增殖），特异活性 >3.86 × 105 IU/mg
保存条件	‑20 °C，避光，12 个月有效期
包装形式	冻干粉，防潮防光包装

规格与采购

规格	单价 (CNY)	库存状态 / 发货时效	备注
5 µg	520	现货（次日发货）
20 µg	1 498	现货（次日发货）
100 µg	3 498	现货（次日发货）
1 mg	请咨询	请咨询	可根据需求提供批量优惠

存储与运输

收到产品后请立即转入‑20 °C 冰箱保存，避免光照。为确保活性，建议在运输全过程使用蓝色冰袋进行冷链配送，且在到货后尽快转冻保存。

使用注意与合规声明

本品仅供科研使用，不适用于临床诊断或治疗。使用本产品时，请确保遵守相关专利、许可证及当地法规。如因使用本产品导致的任何专利侵权或其他违规行为，本公司不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Human PDGF-BB (Platelet-Derived Growth Factor-BB) Recombinant Protein (Catalog Number: PRP1958) is a high-purity research reagent developed for the cytokine and protein core product line, suitable for functional assays in human cell models. The product is produced using an E. coli expression system and is provided as a lyophilized powder for long-term storage and rapid reconstitution.

Key Features

• Molecular Weight: 12.3 kDa
• Purity: > 98% (as determined by SDS-PAGE)
• Activity Assay: In the Ba/F3-c3T3 cell proliferation assay, the ED₅₀ is approximately 2.588 ng/mL, and the specific activity of the recombinant protein is approximately > 3.86 × 10⁵ IU/mg
• Expression Host: E. coli
• Form: Lyophilized Powder
• Storage Recommendation: Store protected from light at -20°C to ensure a 12-month shelf life.
• Shipping Method: Cold chain (blue ice packs)

Applicable Research Areas

PDGF-BB plays a key role in various cell biology and tissue engineering experiments, commonly used in the following studies:

• Studies on the proliferation, migration, and survival of fibroblasts, smooth muscle cells, and bone cells
• Angiogenesis models and exploration of signaling pathways related to vascular remodeling
• In vitro evaluation of bone regeneration and repair, particularly the synergistic effects with osteoblasts and mesenchymal stem cells
• Hemorrhagic shock models related to hemodynamics and vascular contraction regulation (e.g., CX43-mediated vascular responses)
• Functional studies on corpus cavernosum smooth muscle cells (CCSMCs), focusing on PDGFR-mediated signaling pathways

Technical Parameters

Parameter	Value/Description
Gene ID	5155
Protein ID (Uniprot)	P01127
Target Species	Human
Expression System	Recombinant Expression in E. coli
Purity	>98% (SDS-PAGE)
Activity	ED50 = 2.588 ng/mL (Ba/F3-c3T3 proliferation); Specific Activity >3.86 × 105 IU/mg
Storage Conditions	-20°C, protected from light, 12-month shelf life
Formulation	Lyophilized powder, packaged to protect from moisture and light

Specifications & Ordering

Specification	Unit Price (CNY)	Stock Status / Shipping Time	Note
5 µg	520	In Stock (Ships Next Day)
20 µg	1,498	In Stock (Ships Next Day)
100 µg	3,498	In Stock (Ships Next Day)
1 mg	Please Inquire	Please Inquire	Bulk discounts available upon request

Storage & Shipping

Upon receipt, immediately transfer the product to a -20°C freezer for storage, protected from light. To ensure activity, cold chain shipping using blue ice packs is recommended throughout transit, and the product should be transferred to frozen storage as soon as possible after arrival.

Usage Notes & Compliance Statement

This product is intended for research use only and is not suitable for clinical diagnosis or treatment. When using this product, ensure compliance with relevant patents, licenses, and local regulations. The company assumes no responsibility for any patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Acogen.