IGF-I - Human IGF-I Protein, H

重组人胰岛素样生长因子-I (IGF-I) 蛋白，His标签 (无动物源)

货号：PRP1933

产品概述

本品为高纯度、重组表达的人源胰岛素样生长因子-I (IGF-I) 蛋白，采用无动物源生产工艺，并带有His标签。该蛋白经严格质控，适用于细胞培养等各类生物医学研究，为探索细胞生长、代谢及相关信号通路提供可靠的工具。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 >95%。
• 低内毒素：每微克蛋白内毒素含量 <0.01 EU (LAL法测定)，有效降低对细胞实验的干扰。
• 已验证生物活性：经验证能够诱导MCF-7细胞增殖，ED₅₀范围为 0.9 - 3.1 ng/mL，比活力约 1.2 × 10³ IU/mg。
• 无动物源表达：在大肠杆菌表达系统中生产，避免动物源成分带来的潜在风险。
• 严格的质控：从表达宿主到最终产品，全程进行严格的质量控制。

技术参数

参数	详情
种属来源	人
氨基酸序列	人胰岛素样生长因子Ⅰ (Gly49-Ala118)
表达宿主	大肠杆菌
分子量	~11 kDa
产品形式	冻干粉
冻干缓冲液	无菌PBS, pH 7.4
蛋白长度	全长

生物背景

胰岛素样生长因子-I (IGF-I)，也被称为生长调节素C (Somatomedin C)，是一种主要由肝脏合成的重要生长因子，广泛参与调节细胞增殖、分化、代谢及凋亡等过程。它通过与细胞表面的IGF-I受体结合，激活下游信号通路，从而在个体生长、组织修复以及代谢稳态维持中发挥核心作用。

使用说明

复溶建议：
开盖前请先离心。建议使用提供的复溶缓冲液进行复溶，浓度不低于100 µg/mL，以获得最佳效果。后续可根据具体实验需求进行稀释。

保存条件：
冻干粉可在-20°C下稳定保存1年。复溶后于2-8°C可保存1周。若在含有载体蛋白（如0.1% BSA）的缓冲液中稀释并分装，置于-20°C或-80°C可保存3-6个月。请避免反复冻融。

规格与价格

规格	价格 (人民币)	货期
100 µg	588	现货（次日发货）
500 µg	1498	请咨询
1 mg	1998	请咨询
10 mg	请咨询	请咨询

运输条件：冰袋运输（蓝冰）

警告：本品仅供科研使用，不适用于人类或临床诊断。使用前请查阅所有相关材料安全数据表。用户有责任确保其使用方式符合所有适用的法律法规。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Insulin‑Like Growth Factor‑I (IGF‑I) Protein, His‑Tag (Animal‑Component Free)

Product No.：PRP1933

Product Overview

This item is a high‑purity, recombinantly expressed human insulin‑like growth factor‑I (IGF‑I) protein produced by an animal‑component‑free process and carries a His‑tag. The protein undergoes stringent quality control and is suitable for cell culture and various biomedical research applications, providing a reliable tool for investigating cell growth, metabolism, and related signaling pathways.

Key Features

• High purity：Purity >95 % by SDS‑PAGE analysis.
• Low endotoxin：<0.01 EU per µg protein (LAL assay), minimizing interference in cellular experiments.
• Validated biological activity：Demonstrated to induce proliferation of MCF‑7 cells with an ED₅₀ range of 0.9–3.1 ng/mL and specific activity of ≈1.2 × 10³ IU/mg.
• Animal‑component free expression：Produced in an Escherichia coli expression system, eliminating potential risks associated with animal‑derived components.
• Stringent quality control：Comprehensive QC from host expression through final product.

Technical Specifications

Parameter	Details
Species source	Human
Amino‑acid sequence	Human insulin‑like growth factor‑I (Gly49‑Ala118)
Expression host	Escherichia coli
Molecular weight	~11 kDa
Product form	Lyophilized powder
Lyophilization buffer	Aseptic PBS, pH 7.4
Protein length	Full‑length

Biological Background

Insulin‑like growth factor‑I (IGF‑I), also known as somatomedin C, is a major growth factor primarily synthesized in the liver. It plays a broad role in regulating cell proliferation, differentiation, metabolism, and apoptosis. Binding to the cell‑surface IGF‑I receptor activates downstream signaling pathways, thereby contributing critically to organismal growth, tissue repair, and metabolic homeostasis.

Usage Instructions

Reconstitution recommendation：
Centrifuge before opening the vial. It is recommended to reconstitute with the supplied buffer to a concentration of at least 100 µg/mL for optimal performance. Subsequent dilutions can be made according to experimental requirements.

Storage conditions：
Lyophilized powder is stable at –20 °C for up to 1 year. After reconstitution, store at 2–8 °C for up to 1 week. If diluted and aliquoted in a carrier protein solution (e.g., 0.1 % BSA), samples can be stored at –20 °C or –80 °C for 3–6 months. Avoid repeated freeze‑thaw cycles.

Specifications & Pricing

Specification	Price (CNY)	Lead time
100 µg	588	In stock (next‑day shipment)
500 µg	1498	Please inquire
1 mg	1998	Please inquire
10 mg	Please inquire	Please inquire

Shipping condition：Ice‑pack shipping (blue ice)

Warning：This product is for research use only and is not intended for human or clinical diagnostic applications. Consult all relevant material safety data sheets prior to use. Users are responsible for ensuring that their use complies with all applicable laws and regulations.

Note: The above information is subject to change due to product optimization or upgrades. For the most current details, please contact YaKeen Inc.