88881108赛默飞200ml锥形瓶夹具Thermo Fi

dissociated neurons for patch clamp or Immunohistochemistry

enzyme, to degrade proteins in brain tissue.   This will produce a one cell thick layer of dissociated cells for further studies in patch clamp recording or  immunohistochemistry .     Materials:              Hippocampal brain slices

Lipoprotein Isolation - First week

100 ml beaker and add 25 µl of 200 mM PMSF Caution! Highly poisonous! picture ). Make sure no insect saliva drips into the beaker (have Kleenex ready to wipe off saliva). 3. Gently inject a few hundred microlitres of PBS

Lipoprotein Isolation - First week

3 Lyophilization.   Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean 100 ml beaker and add 25 µl of 200 mM PMSF