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文献和实验Rotor equilibration without counterbalance
Rotor equilibration without counterbalance Contributed by Dr.A.Gratchev This is relatively obvious method, which I found not used by most of the researchers. Usually the equilibration of 2,3,4,6,8,9, and 10 tubes in 12x rotor
FACS Procedures for Apoptosis Detection
20 (40 ) 12x75mm snap cap tubes (Falcon 2054). Controls Required: Cells only (no treatment/no stain) Treated cells with Hoechst only Treated cells with 7-AAD only Untreated cells with both stains Procedure
无可见物质存在。 (9)将上层水相转入一新的eppendorf管,加等体积氯仿,强烈振荡1分钟后,12000g离心5分钟,多次重复此步骤。 (10)将上层水相转入新的eppendorf管,加0.5倍体积的7.5 mol/L 醋酸铵,2倍体积乙醇,混匀后-20℃放置 30分钟。 (11)12000g离心15分钟,去除上清液,用70%乙醇小心清洗沉淀,如果沉淀已被搅起,重新离心,倾去酒精,真空干燥沉淀物。 (12)将DNA 的沉淀物溶解于20ml去离子水中,取2ml样品进行琼脂
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