GM-CSF - Human GM-CSF protein

重组人粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 蛋白

货号：PRP1016 | 产品类型：细胞因子

产品概述：

本品为高纯度、高生物活性的重组人GM-CSF蛋白，无标签，专为细胞培养及相关科研应用设计。该蛋白采用大肠杆菌表达系统制备，具有与天然人源GM-CSF一致的生物功能，可有效促进造血前体细胞的增殖与分化。

关键特性：

• 高纯度：经SDS-PAGE验证，纯度 > 98 %
• 高生物活性：ED₅₀ 范围为 0.05–0.5 ng/mL（TF-1细胞增殖实验）
• 低内毒素：LAL法检测，每 1 μg 蛋白质中 EU 含量 < 0.01
• 无标签设计：避免标签干扰，更贴近天然蛋白结构
• 全长表达：由128个氨基酸组成，分子量约14 kDa

产品信息：

表达宿主	大肠杆菌	种属来源	人
蛋白长度	128 aa（Ala18-Glu144）	分子量	14 kDa
产品形式	冻干粉（PBS，pH 7.4）	保存条件	-20℃ 干燥保存

应用领域：

• 造血干细胞与祖细胞培养
• 粒细胞、巨噬细胞分化研究
• 免疫细胞功能调控实验
• 相关疾病模型构建（如白血病研究）

使用建议：

• 使用前请离心，推荐使用配套复溶缓冲液，浓度不低于 100 µg/mL
• 复溶后可在 4℃ 保存 2–7 天，长期保存请置于 -20℃ 以下
• 为保持稳定性，建议添加载体蛋白（如 0.1% HSA 或 BSA）并分装保存

背景说明：

粒细胞-巨噬细胞集落刺激因子（GM-CSF）是由T细胞、巨噬细胞等多种细胞产生的细胞因子，在造血调控及免疫调节中发挥核心作用。它能刺激粒细胞、巨噬细胞、嗜酸性粒细胞等前体细胞的增殖与成熟，临床上常用于骨髓移植后造血功能恢复及白血病辅助治疗研究。

规格与价格：

规格	价格（元）	货期
5 μg	520	现货，次日发货
20 μg	1498	现货，次日发货
100 μg	4516	现货，次日发货
1 mg	请咨询	请咨询

注意事项：

本产品仅供科研使用，不适用于临床诊断或人体治疗。使用前请查阅相关文献并遵守所在地区的法规要求。

相关资源：

• UniProt 数据库链接：P04141 (GM-CSF)
• 基因ID：1437 | 别名：GM-CSF; GMCSF; CSF2

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein

Catalog Number: PRP1016 | Product Type: Cytokine

Product Overview:

This product is a high-purity, high-bioactivity recombinant human GM-CSF protein, tag-free, specifically designed for cell culture and related research applications. The protein is produced using an E. coli expression system and exhibits biological functions identical to natural human GM-CSF, effectively promoting the proliferation and differentiation of hematopoietic precursor cells.

Key Features:

• High Purity: > 98% purity as verified by SDS-PAGE
• High Biological Activity: ED₅₀ range of 0.05–0.5 ng/mL (TF-1 cell proliferation assay)
• Low Endotoxin: < 0.01 EU per 1 μg of protein as determined by LAL assay
• Tag-Free Design: Avoids tag interference, closer to the native protein structure
• Full-Length Expression: Consists of 128 amino acids, with a molecular weight of approximately 14 kDa

Product Information:

Expression Host	E. coli	Species Source	Human
Protein Length	128 aa (Ala18-Glu144)	Molecular Weight	14 kDa
Form	Lyophilized powder (PBS, pH 7.4)	Storage Conditions	Store dry at -20°C

Applications:

• Culture of hematopoietic stem cells and progenitor cells
• Studies on granulocyte and macrophage differentiation
• Functional modulation experiments of immune cells
• Construction of related disease models (e.g., leukemia research)

Usage Suggestions:

• Centrifuge before use. Reconstitution buffer is recommended at a concentration no less than 100 µg/mL.
• After reconstitution, it can be stored at 4°C for 2–7 days. For long-term storage, store at -20°C or below.
• To maintain stability, the addition of a carrier protein (e.g., 0.1% HSA or BSA) and aliquot storage are recommended.

Background:

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a cytokine produced by various cells, including T cells and macrophages, playing a central role in hematopoietic regulation and immune modulation. It stimulates the proliferation and maturation of precursor cells such as granulocytes, macrophages, and eosinophils. It is commonly used clinically for research on hematopoietic recovery post-bone marrow transplantation and as an adjunct in leukemia treatment studies.

Specifications & Pricing:

Specification	Price (CNY)	Availability
5 μg	520	In stock, ships next day
20 μg	1498	In stock, ships next day
100 μg	4516	In stock, ships next day
1 mg	Please inquire	Please inquire

Note:

This product is intended for research use only and is not suitable for clinical diagnosis or human therapy. Please consult relevant literature and comply with regulatory requirements in your region before use.

Related Resources:

• UniProt Database Link: P04141 (GM-CSF)
• Gene ID: 1437 | Aliases: GM-CSF; GMCSF; CSF2

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult YaKeyin Company for specific details.