重组人核因子κ B受体活化因子配体（RANKL）

重组人核因子κ B受体活化因子配体（RANKL）蛋白

PRP1056 | Huamn RANKL protein

产品概述

本品为大肠杆菌表达的无标签重组人源RANKL蛋白片段（C-末端含6×His标记），专为细胞培养及骨代谢、免疫系统相关研究设计。严格遵循LAL法质控，内毒素含量<0.1 EU/μg，确保实验可靠性。

关键参数

• 分子量：20.67 kDa（理论值）| 17 kDa（SDS-PAGE还原条件）
• 活性验证：ED50<10 ng/mL（RAW264.7细胞破骨分化诱导实验）
• 纯度：>98%（SDS-PAGE银染验证）
• 表达系统：大肠杆菌高效表达体系
• 种属来源：人源（UniProt ID：O14788）

产品优势

• 高批次一致性：严格质控确保蛋白活性稳定性（≥1年-20℃保存）
• 即用型冻干粉：预过滤（0.2 µm）处理，1X PBS pH8.0缓冲体系
• 灵活应用：支持多种缓冲体系稀释（含BSA/FBS/HSA/海藻糖方案）

规格与定价

• 5 μg ￥588 | 20 μg ￥1498 | 100 μg ￥3998（现货次日发）
• 1 mg 定制规格（详询客服获取专属报价）

使用指南

• 复溶建议：无菌10 mM HCl溶解（≥100 μg/mL），室温静置≥20分钟
• 禁忌操作：严禁振荡！离心预处理（3000 rpm ×5 min）
• 保存方案：
  冻干态：-20℃（12个月）
  工作液：2-8℃（1周）/-20℃（3-6个月，含稳定剂体系）

运输与质保

全程蓝冰运输 | 提供完整COA及质控文件

重要声明

研究用途：本产品仅限科研使用，不适用于临床诊断或治疗应用。使用者须自行确认实验方案不侵犯第三方知识产权。

背景信息

RANKL（TNFRSF11A）作为TNF超家族关键成员，通过RANK信号通路调控骨重塑与免疫调节。其317aa结构中，Ala142-Asp317区段包含功能性受体结合域，是研究骨质疏松、癌症骨转移等机制的核心靶点。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Receptor Activator of Nuclear Factor κ B Ligand (RANKL) Protein

PRP1056 | Human RANKL Protein

Product Overview

This product is a tag-free recombinant human RANKL protein fragment (C-terminal 6×His tag) expressed in E. coli, specifically designed for cell culture and research related to bone metabolism and the immune system. Strictly controlled by the LAL method, endotoxin content is <0.1 EU/μg, ensuring experimental reliability.

Key Parameters

• Molecular Weight:20.67 kDa (Theoretical) | 17 kDa (SDS-PAGE under reducing conditions)
• Activity Validation:ED50<10 ng/mL (RAW264.7 cell osteoclast differentiation induction assay)
• Purity:>98% (SDS-PAGE silver staining validation)
• Expression System:High-efficiency E. coli expression system
• Species Source:Human (UniProt ID: O14788)

Product Advantages

• High batch consistency: Strict quality control ensures protein activity stability (≥1 year at -20℃ storage)
• Ready-to-use lyophilized powder: Pre-filtered (0.2 µm) and formulated in 1X PBS pH8.0 buffer system
• Flexible application: Supports dilution in various buffer systems (including BSA/FBS/HSA/trehalose solutions)

Specifications and Pricing

• 5 μg ￥588 | 20 μg ￥1498 | 100 μg ￥3998 (In stock, shipped next day)
• 1 mg Custom specifications (Contact customer service for exclusive quotes)

Usage Guidelines

• Reconstitution Recommendation:Dissolve in sterile 10 mM HCl (≥100 μg/mL), let stand at room temperature for ≥20 minutes
• Prohibited Operations:Do not vortex! Centrifuge pre-treatment (3000 rpm ×5 min)
• Storage Solution:
  Lyophilized state: -20℃ (12 months)
  Working solution: 2-8℃ (1 week)/-20℃ (3-6 months, with stabilizer system)

Shipping and Quality Assurance

Shipped with blue ice throughout | Complete COA and quality control documents provided

Important Statement

Research Use Only:This product is for research use only and is not suitable for clinical diagnosis or therapeutic applications. Users must confirm that the experimental protocol does not infringe on third-party intellectual property rights.

Background Information

RANKL (TNFRSF11A), as a key member of the TNF superfamily, regulates bone remodeling and immune modulation through the RANK signaling pathway. Its 317aa structure includes the Ala142-Asp317 segment, which contains the functional receptor-binding domain and is a core target for studying mechanisms of osteoporosis, cancer bone metastasis, etc.

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Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine Company.