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- Thermo Fisher
- RBA15-16-125
- 2025年12月31日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
, which is enough for two 0.8 mm thick gels, or one 1.5 mm gel.5% 6% 7% 8% 9% 10%5 6 7 8 9 10 ml 30% acrylamide/bis7.5 7.5 7.5 7.5 7.5 7.5 ml 1.5 M Tris 8.817 16 15 14 13 12 ml H2OThen add
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