3599-46赛默飞摇床附件Thermo Fisher UT

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Sigma E7637 Carcinogenic—needs to be disposed of as hazardous waste Glacial acetic acid Fisher BP2401

SDS-PAGE

than more concentrated mixtures.) Stain the gel 20 min in a tray on a rotating platform. Longer staining helps see faint bands, but then you have to destain longer. Pour the used stain down the sink, rinse gel in a little water to get excess stain off. Destain by soaking

【转贴】immunofluorescence staining

of use, make a 10% stock solution of the Triton X-100 in PEM buffer and aliquot to microcentrifuge tubes covered with foil. Store the stocks at -20 C.Coverslips:Round coverslips will fit in 24 well trays, and can be obtained from:Fisher Scientific, Cat