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Divide into two 15 ml c/f tubes--5 ml each. Invert for additional 5 min. Solution should be pink and will contain thread-like material. Spin at 4°C, 4000 rpm for 20 min to remove insoluble material. All spins done in fixed angle rotor

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microscopy, fixation of biological material and staining for contrast enhancement were the standard techniques. The results from freeze-etching, metal shadowing and from ultrathin-sections of plastic-embedded material shaped our view of the cellular

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. However, with greatpower comes great responsibility. Contamination of reagents or test samples with amplifiable material,such as previous reaction products, can be crippling to scientists applying PCR protocols. Prevention of PCR contamination is far and away preferred