88881004赛默飞滚瓶机，可调速，数字显示，可叠放，耐受
CO2， 1-80rpm;100-240,50/60HzThermo Fisher Bottle/Tube Roller; Global version; Variable Speed; Digital Display; Stackable; Humidity CO2 Resistance; 0.5-80rpm; 100-240V, 50/60Hz; EU,UK, AU plugs- ￥16841.44
- Thermo Fisher
- 88881004
- 2025年12月31日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Plant DNA methylation analysis by bisulfite genomic sequencing
K (or equivalent) >15 minutes at 4°C 12000rpm (12000 X g); Discard supernatant, vacuumdry for 10 minutes at room temperatur; Dissolve DNA in 50µl bidist. water (20ng/µl or 200ng/µl, respectively), store at -20°C; PCR
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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