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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
are subbed with poly-lysine on the day of use: 50 µl of poly-lysine solution is allowed to settle on slides for 10-20 min; excess solution is wiped off and slides are baked at 60oC for 10 min. II. PROCEDURE A. Overview. A mixed population of C
in this extraction buffer at minus 20C for greater than a year) 2. Using a Kontes micro-homogenizer with sterilized tips (Fisher Scientific Cat. # K749540-0000) samples are ground into a slurry and incubated for 60 min. at 60C. 3. Salt concentration
1 B-mercaptoethanol (tubes can be stored in this extraction buffer at minus 20C for greater than a year) Using a Kontes micro-homogenizer with sterilized
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