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- 2025年12月31日
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文献和实验O Hemocytometer Hema 3 Stain set (Fisher) Hematocrit (Packed red cell volume) : Draw blood in 2 heparinized hematocrit capillary tubes. Spin 5 min in hematocrit centrifuge. Measure total and packed cell volume. Calculate packed cell volume as a percent
【转贴】immunofluorescence staining
Kit (Molecular Probes), coverslips can (optionally) be incubated with 1-2 drops of Equilibration Buffer for 5-10 min. before mounting.Place a drop of mounting media on the slide for each coverslip to be mounted (two drops for square coverslips
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
Cut sections at 4µm, use a clean water bath with distilled water, and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Superfrost plus cat 12-550-15). [Optional
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