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文献和实验Preparation and Staining of Frozen Tissue Sections
the positioning of the block to align the block with the knife blade. Cut tissue block until the desired tissue is exposed. Cut sections of the desired thickness (usually 5 µm), place the sections on a Fisher Superfrost slide and dry overnight at room tem
and store at 4°C. 3. 2M TEAB stock: To make 500 ml, take 138.3 ml (101 g) TEA and fill to 400 ml with DDW. Use dry ice to bubble in CO2 until pH is below 8.0. Complete to 500 ml with DDW and store at 4°C. *TEA is extremely hazardous so take care
and fill to 400 ml with DDW. Use dry ice to bubble in CO2 until pH is below 8.0. Complete to 500 ml with DDW and store at 4°C. *TEA is extremely hazardous so take care when handling. Use glassware instead of plasticware when measuring and transporting
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