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- Thermo Fisher
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- 2025年12月31日
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
with phenol/chloroform/isoamyl alcohol (25:24:1), spinning 12000g 10 min at 4°C in a fixed angle rotor. Save aqueous (upper) phase; add gd H2 O if volume is less than 2 ml. 12. Add 0.1 vol 3M NaOAc pH 6.0, mix and add 2.5 vol EtOH, mix. Place at -20°C
A quick RNA mini-prep for Neurospora mycelial cultures
at 4°C in a fixed angle rotor. Save aqueous (upper) phase; add gd H2 O if volume is less than 2 ml. 12. Add 0.1 vol 3M NaOAc pH 6.0, mix and add 2.5 vol EtOH, mix. Place at -20°C overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash
to an empty BioRad spin column. Add 1.5 ml TE. Place column in 13 x 100 mm glass test tube. Spin in clinical centrifuge (12-place fixed angle rotor) on setting 2 for ~1 min until all liquid is out of the resin (don't over centrifuge as this may damage the resin
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