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文献和实验at room temp for 5 min. 5. Add 4 ml of cold buffer P3. Cover with parafilm immediately and mix by gentle inversion 4-6 times. Incubate on ice 15 min. 6. Centrifuge in the F0650 rotor, using a blue rubber adapter sleeve, at 20,000 x g for 30 min
Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)
by gentle inversion 4-6 times. Incubate on ice 15 min. 6. Centrifuge in the F0650 rotor, using a blue rubber adapter sleeve, at 20,000 x g for 30 min in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube
Native Chromatin Preparation and Illumina/Solexa Library Construction
1. On the day of the experiment, obtain a leukapheresis pack from a blood bank. Dilute 109 leukocytes with 1X PBS (1:1). Pipette 15 mL of LSM into a 50-mL conical tube. Carefully layer the lymphocyte suspension over the LSM. A clear boundary
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