51900080赛默飞二氧化碳培养箱配件Thermo Fis

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

(37%, Fisher, Cat# F79-500)- Formamide (Boehringer Mannheim Cat# 100 731)- Glycerol (Boehringer Mannheim Cat#100 649)- Glycine (Sigma Cat# G-4392)- Glycogen (Boeringher Mannheim Cat# 901-393)- Heparin (Sigma, Cat# H-3393)- Hepes

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

is incubated for 35 thermal cycles as follows: 95°C for 45 seconds 55°C for 30 seconds (lower temperature for primers less than 20nt) 72°C for 1 minute f. 75 µl of H20 is added to the reaction below the oil and 90 - 95 µl of the diluted reaction

DNA Solutions （DNA分离）

to sterilize (store at 4deg.C). Deionized formamide : Stir formamide (Schwarz/Mann Biotech 800686) with Amberlite MB resin, 10 g. per 100 ml, for one hour to deionize; filter through Whatman 3MM paper, store in a dark bottle at room temperature or 4deg.C. 10