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文献和实验BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
at 5,000 rpm for 15 minutes in RC5-B using GSA rotor, the supernatant is decanted and the pellet drained. 7、The second acetate precipitation step is performed by quickly and gently dissolving the DNA pellet in 3.6 ml of 10:50 TE (10 mM Tris-HCl, pH
, swirl to mix, and incubate in an ice-water bath for 1-2 hours. 8a. Collect the PEG-precipitated DNA by centrifugation in 250 ml bottles at 7000 rpm for 20 minutes at 4degC in the RC5-B using the GSA rotor. 9a. Dissolve the pellets in a combined
A safe method of extracting DNA from Coccidioides immitis
in Microbiological and Biomedical Laboratories, 3rd ed. NIH, Washington). In this paper we describe a safe and convenient method of extracting DNA from such fungi in which the culture is killed by steaming, allowing removal from the containment facilities, as soon
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