RD72赛默飞转头Thermo Fisher ROTOR

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture

DNA纯化和浓缩新方法:超滤

  Amicon Ultra-0.5 mL Centrifugal Filters for DNA Purification and Concentration 操作示意图： With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times

Methods for DNA isolation(DNA分离方法)

of bacteria harboring the plasmid or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100μg/ml)and incubate at 37deg C 8-10 hours with shaking at 250 rpm